Isolation and characterization of six microsatellite loci in the larch budmoth Zeiraphera diniana (Lepidoptera: Tortricidae)

Delamaire, Sophie; Esselink, G.; Samiei, Leila; Courtin, Claudine; Magnoux, Emmanuelle; Rousselet, Jérôme; Smulders, M.J.M.

doi:10.14411/eje.2010.034

Cited by 4 publications

(3 citation statements)

References 27 publications

(17 reference statements)

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“…In this study, we observed a very high number of alleles per locus (average = 13.6) compared to other Lepidoptera species; 2.2 in Helicoverpa zea [ 36 ], 3 in Hyphantria cunea [ 37 ], 4.1 in Helicoverpa armigera [ 36 ], 4.6 in Carposina sasakii [ 38 ], 4.7 in Epirrita autumnata [ 35 ], 4.7 in Plutella xylostella [ 39 ], 7.0 in Chilo suppressalis [ 40 ], 8.8 in Zeiraphera diniana [ 41 ], and 10.5 in Parnassius apollo [ 42 ]. The large number of STR markers detected (2374) in this study enabled the possibility of selection of the most highly polymorphic markers.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

et al. 2018

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The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella. Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden (n = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species.

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Section: Discussionmentioning

confidence: 99%

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

et al. 2018

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“…Although the microsatellite cloning efficiency in Lepidoptera was very low in previous reports (Tsuchida et al , 2003; Zhang, 2004; Endersby et al , 2005; Petenian et al , 2005; Takahashi et al , 2005; Ishiguro & Tsuchida, 2006; Delamaire et al , 2010), we did not encounter a problem with cloning efficiency. The microsatellite cloning efficiency averaged 19.75% in this study, and was highest in (AC) 15 libraries (32.92%).…”

Section: Discussionmentioning

confidence: 55%

Characterization of novel microsatellite markers forHyphantria cuneaand implications for other Lepidoptera

Cao

Wen

Wei

et al. 2015

Bull. Entomol. Res.

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This is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers for H. cunea from SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified as Grouped should not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy-Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, and F ST performed better than did R ST for the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies of H. cunea.

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“…Although microsatellites are the most popular molecular marker in population genetics, it is difficult and challenging to develop microsatellites for many lepidopteran insects (Delamaire et al , 2010), because microsatellite sequences usually exist in multiple copies in the genome, and have similar or almost identical flanking regions in lepidopteran genomics (Meglécz et al , 2004; Sinama et al , 2011). Mobile elements (e.g.…”

Section: Discussionmentioning

confidence: 99%

New microsatellites revealed strong gene flow among populations of a new outbreak pest,Athetis lepigone(Möschler)

Zhu

Sun

Dai

et al. 2017

Bull. Entomol. Res.

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Athetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a new outbreak pest in China. Consequently, it is unclear whether the emergence and spread of the outbreak of this pest are triggered by rapid in situ population size increases in each outbreak area, or by immigrants from a potential source area in China. In order to explore the outbreak process of this pest through a population genetics approach, we developed ten novel polymorphic expressed sequence tags (EST)-derived microsatellites. These new microsatellites had moderately high levels of polymorphism in the tested population. The number of alleles per locus ranged from 3 to 19, with an average of 8.6, and the expected heterozygosity ranged from 0.269 to 0.783. A preliminary population genetic analysis using these new microsatellites revealed a lack of population genetic structure in natural populations of A. lepigone. The estimates of recent migration rate revealed strong gene flow among populations. In conclusion, our study developed the first set of EST-microsatellite markers and shed a new light on the population genetic structure of this pest in China.

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Isolation and characterization of six microsatellite loci in the larch budmoth Zeiraphera diniana (Lepidoptera: Tortricidae)

Cited by 4 publications

References 27 publications

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

Characterization of novel microsatellite markers forHyphantria cuneaand implications for other Lepidoptera

New microsatellites revealed strong gene flow among populations of a new outbreak pest,Athetis lepigone(Möschler)

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