Characterization of novel microsatellite markers forHyphantria cuneaand implications for other Lepidoptera

Abstract

This is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers for H. cunea from SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified as Grouped should not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy-Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, and F ST performed better than did R ST for the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies of H. cunea.

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

“…In this study, we observed a very high number of alleles per locus (average = 13.6) compared to other Lepidoptera species; 2.2 in Helicoverpa zea [ 36 ], 3 in Hyphantria cunea [ 37 ], 4.1 in Helicoverpa armigera [ 36 ], 4.6 in Carposina sasakii [ 38 ], 4.7 in Epirrita autumnata [ 35 ], 4.7 in Plutella xylostella [ 39 ], 7.0 in Chilo suppressalis [ 40 ], 8.8 in Zeiraphera diniana [ 41 ], and 10.5 in Parnassius apollo [ 42 ]. The large number of STR markers detected (2374) in this study enabled the possibility of selection of the most highly polymorphic markers.…”

Development and Characterization of Novel Microsatellite Markers for the Peach Fruit Moth Carposina sasakii (Lepidoptera: Carposinidae) Using Next-Generation Sequencing

“…Heterozygote deficiency can be caused by the Wahlund Effect [ 31 ] or the presence of null alleles, for which Lepidoptera species are notorious [ 32 , 33 , 34 , 35 , 36 ]. It was considered that the present of null alleles is very common in this order due to the flanking region with repetitive sequences and multiple copies of loci [ 37 , 38 , 39 ]. Random sequences of C .…”

Bulk development and stringent selection of microsatellite markers in the western flower thrips Frankliniella occidentalis

“…Eight of these loci exhibited an SNI. SNI mutation patterns can be complex, involving insertion/deletion (indel) events in flanking regions or may result from an excess of base substitutions 32 . Although these alleles can be identified manually, it is laborious and inaccurate.…”