Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

Tan, Chia Wanq; Rukayadi, Yaya; Hasan, Hanan; Abdul-Mutalib, Noor-Azira; Jambari, Nuzul Noorahya; Hara, Hirofumi; Thung, Tze Young; Lee, Epeng; Radu, Son

doi:10.3389/fmicb.2021.616548

Cited by 33 publications

(22 citation statements)

References 73 publications

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“…Moreover, some phages produce plaques surrounded with halos. Halos are ascribed to the diffusion of bacteriophage enzymes that are mostly active against the cell wall of bacterial cells and the biofilms formed by some ( Dakheel et al., 2019 ; Vukotic et al., 2020 ; Tan et al, 2021 ). Observing F2 plaques, they were clear, circular with regular entire margins, and small in diameter (3–5 mm), suggesting it being lytic ( Duc et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Abd-Allah

El-Housseiny

Alshahrani

et al. 2022

Front. Cell. Infect. Microbiol.

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Accumulating evidence has denoted the danger of resistance in tenacious organisms like methicillin-resistant Staphylococcus aureus (MRSA). MRSA, a supple bacterium that adopts a variety of antibiotic resistance mechanisms, is the cause of multiple life-threatening conditions. Approaching a post-antibiotic era, bacteria-specific natural predators, bacteriophages, are now given the chance to prove eligible for joining the antibacterial weaponry. Considering the foregoing, this study aimed at isolating bacteriophages with promising anti-MRSA lytic activity, followed by characterization and optimization of the production of the bacteriophage with the broadest host range. Five phages were isolated from different environmental sources including the rinse of raw chicken egg, raw milk, and, remarkably, the raw meat rinses of chicken and fish. Examined for lytic activity against a set of 23 MRSA isolates collected from various clinical specimens, all five phages showed relatively broad host ranges with the bacteriophage originally isolated from raw fish rinse showing lytic activity against all the isolates tested. This phage is suggested to be a member of Siphoviridae family, order Caudovirales, as revealed by electron microscopy. It also exhibited good thermal stability and viability at different pH grades. Moreover, it showed reasonable stability against UV light and all viricidal organic solvents tested. Optimization using D-optimal design by response surface methodology was carried out to enhance the phage yield. The optimum conditions suggested by the generated model were a pH value of 7, a carbon source of 0.5% w/v sucrose, and a nitrogen source of 0.1% w/v peptone, at a temperature of 28°C and a bacterial inoculum size of 107 CFU/ml, resulting in a 2 log-fold increase in the produced bacteriophage titer. Overall, the above findings indicate the lytic ability inflicted by this virus on MRSA. Apparently, its stability under some of the extreme conditions tested implies its potential to be a candidate for pharmaceutical formulation as an anti-MRSA therapeutic tool. We hope that bacteriophages could tip the balance in favor of the human front in their battle against multidrug-resistant pathogens.

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Section: Discussionmentioning

confidence: 99%

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Abd-Allah

El-Housseiny

Alshahrani

et al. 2022

Front. Cell. Infect. Microbiol.

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“…We detected a total of seven intact prophage sequences in the V. penaeicida chromosomes; PHAGE_Vibrio_12B12 belongs to the family Myoviridae , whereas PHAGE_Escher_ArgO145 and PHAGE_Shigel_POCJ13 belong to the family Podoviridae as per the Virus-Host Database [ 82 ]. In other studies, prophages belonging to the family Podoviridae were detected in V. parahaemolyticus [ 83 ] and Vibrio alginolyticus [ 84 ]. The majority of CDSs of the predicted prophages encoded unknown hypothetical proteins and it is unclear whether the prophages contribute to the virulence of this bacterium.…”

Section: Resultsmentioning

confidence: 99%

“…In other studies, prophages belonging to the family Podoviridae were detected in V. parahaemolyticus [83] and Vibrio alginolyticus [84]. The majority of CDSs of the predicted prophages encoded unknown hypothetical proteins and it is unclear whether the prophages contribute to the virulence of this bacterium.…”

Section: Resultsmentioning

confidence: 99%

Comparative genome analyses of five Vibrio penaeicida strains provide insights into their virulence-related factors

et al. 2022

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Vibrio penaeicida (family Vibrionaceae ) is an important bacterial pathogen that affects Japanese shrimp aquaculture. Only two whole-genome sequences of V. penaeicida are publicly available, which has hampered our understanding of the pathogenesis of shrimp vibriosis caused by this bacterium. To gain insight into the genetic features, evolution and pathogenicity of V. penaeicida, we sequenced five V. penaeicida strains (IFO 15640T, IFO 15641, IFO 15642, TUMSAT-OK1 and TUMSAT-OK2) and performed comparative genomic analyses. Virulence factors and mobile genetic elements were detected. Furthermore, average nucleotide identities (ANIs), clusters of orthologous groups and phylogenetic relationships were evaluated. The V. penaeicida genome consists of two circular chromosomes. Chromosome I sizes ranged from 4.1 to 4.3 Mb, the GC content ranged from 43.9 to 44.1 %, and the number of predicted protein-coding sequences (CDSs) ranged from 3620 to 3782. Chromosome II sizes ranged from 2.2 to 2.4 Mb, the GC content ranged from 43.5 to 43.8 %, and the number of predicted CDSs ranged from 1992 to 2273. All strains except IFO 15641 harboured one plasmid, having sizes that ranged from 150 to 285 kb. All five genomes had typical virulence factors, including adherence, anti-phagocytosis, flagella-related proteins and toxins (repeats-in-toxin and thermolabile haemolysin). The genomes also contained factors responsible for iron uptake and the type II, IV and VI secretion systems. The genome of strain TUMSAT-OK2 tended to encode more prophage regions than the other strains, whereas the genome of strain IFO 15640T had the highest number of regions encoding genomic islands. For comparative genome analysis, we used V. penaeicida (strain CAIM 285T) as a reference strain. ANIs between strain CAIM 285T and the five V. penaeicida strains were >95 %, which indicated that these strains belong to the same species. Orthology cluster analysis showed that strains TUMSAT-OK1 and TUMSAT-OK2 had the greatest number of shared gene clusters, followed by strains CAIM 285T and IFO 15640T. These strains were also the most closely related to each other in a phylogenetic analysis. This study presents the first comparative genome analysis of V. penaeicida and these results will be useful for understanding the pathogenesis of this bacterium.

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“…Phages are found most abundantly in aquatic environments worldwide (Tan et al 2021 ). Temperate phages can integrate into bacterial chromosomes with carried genes and drive bacterial genome evolution (Garin-Fernandez and Wichels 2020 ; Nuidate et al 2021 ; Wendling et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25

Yang

Wang

et al. 2022

Curr Genet

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Vibrio parahaemolyticus is a waterborne pathogen that can cause acute gastroenteritis, wound infection, and septicemia in humans. The molecular basis of its pathogenicity is not yet fully understood. Phages are found most abundantly in aquatic environments and play a critical role in horizontal gene transfer. Nevertheless, current literature on biological roles of prophage-encoded genes remaining in V. parahaemolyticus is rare. In this study, we characterized one such gene VpaChn25_0734 (543-bp) in V. parahaemolyticus CHN25 genome. A deletion mutant ΔVpaChn25_0734 (543-bp) was obtained by homologous recombination, and a revertant ΔVpaChn25_0734-com (543-bp) was also constructed. The ΔVpaChn25_0734 (543-bp) mutant was defective in growth and swimming mobility particularly at lower temperatures and/or pH 7.0–8.5. Cell surface hydrophobicity and biofilm formation were significantly decreased in the ΔVpaChn25_0734 (543-bp) mutant (p < 0.05). Based on the in vitro Caco-2 cell model, the deletion of VpaChn25_0734 (543-bp) gene significantly reduced the cytotoxicity of V. parahaemolyticus CHN25 to human intestinal epithelial cells (p < 0.05). Comparative secretomic and transcriptomic analyses revealed a slightly increased extracellular proteins, and thirteen significantly changed metabolic pathways in the ΔVpaChn25_0734 (543-bp) mutant, showing down-regulated carbon source transport and utilization, biofilm formation, and type II secretion system (p < 0.05), consistent with the observed defective phenotypes. Taken, the prophage-encoded gene VpaChn25_0734 (543-bp) enhanced V. parahaemolyticus CHN25 fitness for survival in the environment and the host. The results in this study facilitate better understanding of pathogenesis and genome evolution of V. parahaemolyticus, the leading sea foodborne pathogen worldwide.

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Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

Cited by 33 publications

References 73 publications

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization

Comparative genome analyses of five Vibrio penaeicida strains provide insights into their virulence-related factors

Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25

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