Comparative genome analyses of five Vibrio penaeicida strains provide insights into their virulence-related factors

Abstract

Vibrio penaeicida (family Vibrionaceae ) is an important bacterial pathogen that affects Japanese shrimp aquaculture. Only two whole-genome sequences of V. penaeicida are publicly available, which has hampered our understanding of the pathogenesis of shrimp vibriosis caused by this bacterium. To gain insight into the genetic features, evolution and pathogenicity of V. penaeicida, we sequenced five V. penaeicida strains (IFO 15640T, IFO 15641, IFO 15642, TUMSAT-OK1 and TUMSAT-OK2) and performed comparative genomic analyses. Virulence factors and mobile genetic elements were detected. Furthermore, average nucleotide identities (ANIs), clusters of orthologous groups and phylogenetic relationships were evaluated. The V. penaeicida genome consists of two circular chromosomes. Chromosome I sizes ranged from 4.1 to 4.3 Mb, the GC content ranged from 43.9 to 44.1 %, and the number of predicted protein-coding sequences (CDSs) ranged from 3620 to 3782. Chromosome II sizes ranged from 2.2 to 2.4 Mb, the GC content ranged from 43.5 to 43.8 %, and the number of predicted CDSs ranged from 1992 to 2273. All strains except IFO 15641 harboured one plasmid, having sizes that ranged from 150 to 285 kb. All five genomes had typical virulence factors, including adherence, anti-phagocytosis, flagella-related proteins and toxins (repeats-in-toxin and thermolabile haemolysin). The genomes also contained factors responsible for iron uptake and the type II, IV and VI secretion systems. The genome of strain TUMSAT-OK2 tended to encode more prophage regions than the other strains, whereas the genome of strain IFO 15640T had the highest number of regions encoding genomic islands. For comparative genome analysis, we used V. penaeicida (strain CAIM 285T) as a reference strain. ANIs between strain CAIM 285T and the five V. penaeicida strains were >95 %, which indicated that these strains belong to the same species. Orthology cluster analysis showed that strains TUMSAT-OK1 and TUMSAT-OK2 had the greatest number of shared gene clusters, followed by strains CAIM 285T and IFO 15640T. These strains were also the most closely related to each other in a phylogenetic analysis. This study presents the first comparative genome analysis of V. penaeicida and these results will be useful for understanding the pathogenesis of this bacterium.

Genomic characterization and identification of virulence‐related genes in Vibrio nigripulchritudo isolated from white leg shrimp Penaeus vannamei

“…All three of the V. nigripulchritudo strains in this study were found to have thermolabile haemolysin (tlh) in chromosome II, which is known to have haemolytic activity in infected shrimps. Tlh has also been reported in V. cholerae ( 019 Xu et al, 2019b), V. harveyi (Deng et al, 2019) and V. penaeicida (Ragab et al, 2022).…”

“…cholerae ( 019 Xu et al, 2019b), V . harveyi (Deng et al, 2019) and V. penaeicida (Ragab et al, 2022).…”

“…Type II secretion system is found in chromosome I of the isolated strains, and it was also found in V . penaeicida genome (Ragab et al, 2022). Other secretion systems, including the VAS type VI secretion system, which secretes lethal VAS effector proteins (Hood et al, 2010), are in chromosome II of the isolated strains.…”

“…In pathogenic bacteria, the extracellular protein secretion (EPS) type II secretion system is mediated by the genes epsC-epsN (Abendroth et al, 2009). Type II secretion system is found in chromosome I of the isolated strains, and it was also found in V. penaeicida genome (Ragab et al, 2022).…”