Isolation and characterization of preantral follicles from foetal bovine ovaries

Hulshof, S. C. J.; Figueiredo, José Ricardo de; Beckers, Jean‐François; Bevers, M.M.; Hurk, R. van den

doi:10.1080/01652176.1994.9694423

Cited by 91 publications

(61 citation statements)

References 11 publications

(14 reference statements)

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“…Other studies showed similar results in the isolation of preantral follicles using a tissue chopper [2,4,34]. In contrast, Hulshof et al [15], using watch maker's forceps to isolate preantral follicles, observed a lower percentage of isolated primordial follicles (12.5%) when compared to primary (57.2%) and secondary (20.7%) follicles. These authors suggest that it may be due to primordial follicles being tightly embedded in the tunica albuginea [15], and a mild mechanical treatment is not sufficient to isolate these follicles.…”

Section: Discussionmentioning

confidence: 78%

“…Other researchers isolated a large number of ovarian preantral follicles from European cows [3,15,24], goats [4,6], ewes [2,5], pigs [25], domestic cats [26,27] and nondomestic felids [28,29], rabbits [30], rats [31], mice [32], and humans [33], using mechanical [2^1, 15,24,[26][27][28][29] or enzymatic [25,[30][31][32][33] procedures. Comparisons between our results and those of other studies are difficult to perform due to species differences, variation in follicle classification, and types of follicles included in the final count.…”

Section: Discussionmentioning

confidence: 99%

“…The isolated preantral follicles were classified into three developmental stages by their morphological appearance according to Hulshof et al [15], i.e. primordial follicles are oocytes surrounded by one layer of flattened or flattened-cubical granulosa cells; primary follicles have a single layer of cubical granulosa cells, and secondary follicles are oocytes surrounded by two or more layers of cubical granulosa cells.…”

Section: Experiments 1: Isolation O F Preantral Folliclesmentioning

confidence: 99%

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation methodCarolina Madeira Lucci Rodolfo Rumpf José Ricardo Figueiredo Sonia Nair Béo AbstractPreantral follicles are a major source of oocytes, and their utilization as an important tool to store large number of female gametes for future use in reproductive programs has been investigated. The increasing importance of studies in this subject, together with the important role of Zebu cattle in the economy of tropical and subtropical countries as well as their well-known differences from European cattle, led to this research. The present study aims to determine the best size interval for sectioning ovarian tissue to isolate preantral follicles from Zebu cows using a tissue chopper and to evaluate the follicular quality after isolation. Furthermore, it aims to provide information about the Zebu cow preantral follicle population and use this data (as a control) to evaluate the effectiveness of the tested isolation method. Testing eight different tissue sectioning size intervals, it was possible to conclude that the 125-pm-section interval is shown to be better than the intervals of 25, 50, 175 and 200 pm to isolate preantral follicles from Zebu cow ovaries. The 125-pm interval allowed the recovery of 26,050±1611 (meaniS.E.M.) preantral follicles per one-half ovary, while the number of preantral follicles in situ estimated by evaluation of histological sections was 35,28812342 per one-half ovary. Thus, the mean (iS.E.M.) recovery rate (=[number of preantral follicles isolated/number of preantral follicles in situ in the same ovary] x 100) was 74.3±4.3%. The morphometrical analysis showed that Bos indicus preantral follicles are similar to B. taurus preantral follicles based on previous reports. In conclusion, this study showed that a simple, mechanical method can be used effectively to isolate a large number of intact preantral follicles from Zebu cow ovaries, with a high recovery rate.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Experiments 1: Isolation O F Preantral Folliclesmentioning

confidence: 99%

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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“…A small fragment of each ovarian piece was removed for electron microscopy. The remainder was fixed in Carnoy fixative for 4 h, [7]. Follicle quality was concurrently evaluated.…”

Section: Histological Analysismentioning

confidence: 99%

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian folliclesThe maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 or 20 °C for 6, 12, or 18 h. Preantral follicles were evaluated by histology and transmission electron microscopy. Storage of ovarian pieces at 20 °C for 12 or 18 h significantly reduced the percentage of morphologically normal follicles compared to controls. In contrast, conservation at 4 °C for up to 18 h and at 20 °C for up to 6 h kept the percentage of normal follicles similar to controls. However, the type of solution that the ovaries were immersed in had little effect on the results. Decreased cellular metabolism probably accounted for better preservation of preantral follicles at 4 °C. In conclusion, zebu cow ovaries were successfully stored at 4 °C for up to 18 h with no morphological damage to preantral follicles. However, at 20 °C, ovaries could only be stored for 6 h.

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“…Sections of 7 μm were stained with periodic acid Schiff (PAS) and haematoxylin. Preantral follicles were classified by their morphological appearance, according to Hulshof et al (1994). The diameters of the follicles and the oocytes were determined when the nucleolus of the oocyte was observed (equatorial section), using an ocular micrometer and considering the smaller diameters.…”

Section: Light Microscopymentioning

confidence: 99%

Light microscopical and ultrastructural characterization of goat preantral follicles

Lucci

Silva

Carvalho

et al. 2001

Small Ruminant Research

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Light microscopical and ultrastructural characterization of goat preantral follicles AbstractGoat ovarian preantral follicles were morphologically and ultrastructurally described in this work. Primordial follicles are oocytes surrounded by one layer of squamous or squamouscuboidal granulosa cells; primary follicles have a single layer of cuboidal granulosa cells, and secondary follicles are oocytes surrounded by two or more layers of cuboidal granulosa cells. At all developmental stages a thick layer of glycoproteins, the basement membrane, surrounded the preantral follicles. The quiescent oocyte is spherical or oval and it has a large eccentrically located nucleus with a conspicuous nucleolus. The organelles were uniformly distributed in the cytoplasm. A large number of vesicles were spread throughout the cytoplasm in all the oocytes. The cytoplasm of oocytes also contains numerous rounded mitochondria besides the usual organelles. As the follicle develops, the mitochondria become elongated. The communication between the oocyte and the granulosa cells is apparently mediated through endocytosis as indicated by the abundant coated pits and vesicles noted in the cortical cytoplasm of the oocyte. The oocyte plasma membrane presented projections that penetrated between adjacent granulosa cells and a few short microvilli lying parallel to the oocyte surface. In secondary follicles, patches of zona pellucida material were observed. Overall, the results indicate that the morphological and ultrastructural organization of caprine preantral follicles resembles that of other mammals. However, some particularities were observed, and that may indicate species specific differences.

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Isolation and characterization of preantral follicles from foetal bovine ovaries

Cited by 91 publications

References 11 publications

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

Light microscopical and ultrastructural characterization of goat preantral follicles

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