Light microscopical and ultrastructural characterization of goat preantral follicles

Lucci, Carolina Madeira; Silva, R.V; Carvalho, Cesar Alexandre Fabrega; Figueiredo, Raimundo Wilane de; Bao, Nirong

doi:10.1016/s0921-4488(01)00196-1

Cited by 67 publications

(74 citation statements)

References 41 publications

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“…primordial, primary and secondary). Also, our results were similar to those described for humans [39], but different from those described for ewes [40] and goats [41]. In addition, some polyovular follicles could be observed in Zebu cow ovaries.…”

Section: Discussionsupporting

confidence: 65%

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation methodCarolina Madeira Lucci Rodolfo Rumpf José Ricardo Figueiredo Sonia Nair Béo AbstractPreantral follicles are a major source of oocytes, and their utilization as an important tool to store large number of female gametes for future use in reproductive programs has been investigated. The increasing importance of studies in this subject, together with the important role of Zebu cattle in the economy of tropical and subtropical countries as well as their well-known differences from European cattle, led to this research. The present study aims to determine the best size interval for sectioning ovarian tissue to isolate preantral follicles from Zebu cows using a tissue chopper and to evaluate the follicular quality after isolation. Furthermore, it aims to provide information about the Zebu cow preantral follicle population and use this data (as a control) to evaluate the effectiveness of the tested isolation method. Testing eight different tissue sectioning size intervals, it was possible to conclude that the 125-pm-section interval is shown to be better than the intervals of 25, 50, 175 and 200 pm to isolate preantral follicles from Zebu cow ovaries. The 125-pm interval allowed the recovery of 26,050±1611 (meaniS.E.M.) preantral follicles per one-half ovary, while the number of preantral follicles in situ estimated by evaluation of histological sections was 35,28812342 per one-half ovary. Thus, the mean (iS.E.M.) recovery rate (=[number of preantral follicles isolated/number of preantral follicles in situ in the same ovary] x 100) was 74.3±4.3%. The morphometrical analysis showed that Bos indicus preantral follicles are similar to B. taurus preantral follicles based on previous reports. In conclusion, this study showed that a simple, mechanical method can be used effectively to isolate a large number of intact preantral follicles from Zebu cow ovaries, with a high recovery rate.

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Section: Discussionsupporting

confidence: 65%

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

et al. 2002

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“…The results showed that the mean diameter of zebu oocytes and follicles and the mean number of granulosa cells at the equatorial section were similar to those observed for European cattle (Van Wezel and Rodgers, 1996, Braw-Tal and Yossefi, 1997and Hyttel et al, 1997 in all three developmental stages evaluated (i.e., primordial, primary and small secondary follicles). Also, our results were similar to those described for humans (Lintern-Moore et al, 1974), but different from those described for ewes (Lundy et al, 1999) and goats (Lucci et al, 2001). …”

Section: Discussionsupporting

confidence: 65%

“…The most important difference observed concerned the mitochondria. Round mitochondria are commonly found in oocytes, and were described in European cattle ( Fair et al, 1997a andHyttel et al, 1997) and goats ( Lucci et al, 2001), especially in primordial and primary follicles.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been done in order to describe the oocyte and follicular structure of several domestic species, including sheep (Turnbull et al, 1977, Cran et al, 1980and Cahill and Mauléon, 1981, goats (Lucci et al, 2001), cats (Jewgenow and Göritz, 1995), dogs (Durrant et al, 1998), pigs (Greenwald and Moor, 1989) and cattle (Hulshof et al, 1994, Beckers et al, 1996and Van Wezel and Rodgers, 1996Fair et al, 1997a andFair et al, 1997b;Hyttel et al, 1997). These studies have shown that many characteristics are similar among species, but others may differ.…”

Section: Introductionmentioning

confidence: 99%

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Morphometric and ultrastructural characterization of Bos indicus preantral follicles

Kacinskis¹,

Lucci²,

Luque³

et al. 2005

Animal Reproduction Science

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Morphometric and ultrastructural characterization of Bos indicus preantral folliclesMirella Ávila Kacinskis Carolina Madeira Lucci Maria Carolina Aquino Luque Sônia Nair Báo AbstractThe aim of the present study was to characterize the ultrastructure of zebu cow preantral follicles (PAFs). Ovarian cortex samples were processed for light and transmission electron microscopy. Primordial follicles consisted of an oocyte surrounded by one layer of flattened or flattened-cuboidal granulosa cells. The oocyte contained a large and usually eccentric nucleus. Most organelles were located at the perinuclear ooplasm. Round shaped mitochondria, which contained electron-dense granules, smooth and rough endoplasma reticulum and a Golgi apparatus were also observed. Vesicles and coated pits were often observed in the cortical ooplasm. In primary follicles, the oocyte was surrounded by one layer of cuboidal granulosa cells. Short microvilli were observed on the oolema. Secondary follicles consisted of an oocyte surrounded by a variable number of layers of cuboidal granulosa cells. Small secondary follicles had an ultrastructure very similar to that observed in primary follicles. At this follicular stage, the zona pellucida was beginning to form around the oocyte. In large secondary follicles, the zona pellucida was totally developed around the oocyte. Several granulosa cell projections could be detected that were encroaching into the zona pellucida and protruding towards the oocyte, where gap junctions were observed between oocyte and granulosa cell membranes. Organelles within the oocyte were located at the periphery of the ooplasm, and clusters of cortical granules were observed. Round mitochondria were abundant in all developmental stages. In conclusion, this study described the ultrastructure of zebu cow PAFs, and some unique characteristics could be observed as compared with what has been reported for follicles of Bos taurus cattle.

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“…Primordial follicles are quiescent, whereas primary follicles are already activated and in early growth. Although the mechanisms of primordial follicle activation are yet undefined, it is well known that oocyte and granulosa cells from primary follicles undergo fast and substantial transformations, such as granulosa cell proliferation and change in shape, oocyte growth and proliferation of cytoplasmic organelles in both cell types [22], [23] and [24]. Cells undergoing such transformations may be more sensitive to environmental factors, and thus degenerate under conditions of reduced temperature.…”

Section: Discussionmentioning

confidence: 99%

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian folliclesThe maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 or 20 °C for 6, 12, or 18 h. Preantral follicles were evaluated by histology and transmission electron microscopy. Storage of ovarian pieces at 20 °C for 12 or 18 h significantly reduced the percentage of morphologically normal follicles compared to controls. In contrast, conservation at 4 °C for up to 18 h and at 20 °C for up to 6 h kept the percentage of normal follicles similar to controls. However, the type of solution that the ovaries were immersed in had little effect on the results. Decreased cellular metabolism probably accounted for better preservation of preantral follicles at 4 °C. In conclusion, zebu cow ovaries were successfully stored at 4 °C for up to 18 h with no morphological damage to preantral follicles. However, at 20 °C, ovaries could only be stored for 6 h.

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Light microscopical and ultrastructural characterization of goat preantral follicles

Cited by 67 publications

References 41 publications

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

Zebu (Bos indicus) ovarian preantral follicles: morphological characterization and development of an efficient isolation method

Morphometric and ultrastructural characterization of Bos indicus preantral follicles

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

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