Isolation and Characterization of Polyester-Based Plastics-Degrading Bacteria from Compost Soils

Sriyapai, Pichapak; Chansiri, Kosum; Sriyapai, Thayat

doi:10.1134/s0026261718020157

Cited by 43 publications

(25 citation statements)

References 31 publications

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“…Phylogenetic analyses showed that the three potential Actinobacteria isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4), the type strain A. keratinilytica WCC-2265 T , and A. miaoliensis BC 44T-5 T formed a clade thermophilic xylandegrading species, supported by the NJ, ME, and ML methods with 68% bootstrap values (Figure 2). Other strains from these two species were also reported as xylanase-producing bacteria by Sriyapai et al (2018) and Taibi et al (2012). The ability of the three Actinobacteria isolates used in this study (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) and the two Actinomadura species (A. keratinilytica and A. miaoliensis) to degrade xylan at high temperatures indicated their potential contribution in organic matter decomposition in nature.…”

Section: Phylogenetic Tree Analysesmentioning

confidence: 57%

“…In addition, some scientists reported that Actinomadura keratinilytica was also a potential producer of other enzymes, e.g. cellulase (Sriyapai et al 2018), pectate lyase (Saoudi et al 2015), keratinase (Habbeche et al 2014), and amylase (Sukkhum et al 2011), while A. miaoliensis was a potential producer of cellulase (Sriyapai et al 2018). Based on this finding, the three potential isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) are potential as xylanase-producing Actinobacteria.…”

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

“…The Actinomadura species used in the phylogenetic tree (Figure 2), were mostly isolated from soil (Tomita et al 1989;Wink et al 2003;Lee and Jeong 2006;Tseng et al 2009;Trujillo and Goodfellow 2012;Zucchi et al 2013;Zhao et al 2015;Malisorn et al 2018); some species were associated with plants (Rachniyom et al 2015;Rachniyom et al 2018); one found in biofilters consisting of tree bark compost (Songsumanus et al 2016); and some were pathogens to animals, including humans (Trujillo and Goodfellow 2012). Information on xylanase-producing Actinomadura species remains limited, although various species, such as A. keratinilytica Cpt20 (Taibi et al 2012), A. miaoliensis TF1 (Sriyapai et al 2018), A. xylanilytica BK147 T (Zucchi et al 2013), A. rudentiformis HMC1 T (le Roes and Meyers 2007), and A. kijaniata SCC 1256 T (Horan and Brodsky 1982), have been reported to display xylanolytic activity. Some Actinomadura species produced other enzymes, such as cellulase Goodfellow 2012, Sriyapai et al 2018), amylase (Horan and Brodsky 1982;Lee and Jeong 2006;Sazak et al 2012;Trujillo and Goodfellow 2012;Rachniyom et al 2015;Zhao et al 2015;Abagana et al 2016;Songsumanus et al 2016;Rachniyom et al 2018), gelatinase (Horan and Brodsky 1982;Mertz and Yao 1986;Zucchi et al 2013), and keratinase (Puhl et al 2009;Trujillo and Goodfellow 2012;Habbeche et al 2014;…”

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.

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Section: Phylogenetic Tree Analysesmentioning

confidence: 57%

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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“…Then, as the hydrolysis process progress, PLAbased samples decrease in size evidenced by a reduction of the sample thickness accompanied with an increase in PLA fragility (Arrieta, Fortunati, et al, 2014a;Siracusa, Rocculi, Romani, & Rosa, 2008). Several bacterial strains, for instance those belonging to the genera Actinomadura, Streptomyces and Laceyella, utilize polyesters as a carbon source having good polyester-degrading activity (Sriyapai, Chansiri, & Sriyapai, 2018). Depolymerase enzymes (i.e.…”

Section: Pla Wastesmentioning

confidence: 99%

Influence of plasticizers on the compostability of polylactic acid

Arrieta

2021

J APPL RES TECH ENG

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<p>Poly(lactic acid) (PLA) has gained considerable attention as an interesting biobased and biodegradable polymer for film for food packaging applications, due to its many advantages such as biobased nature, high transparency and inherent biodegradable/compostable character. With the dual objective to improve PLA processing performance and to obtain flexible materials, plasticizer are use as strategy for extending PLA applications as compostable film for food packaging applications. Several plasticizers (i.e.: citrate esters, polyethylene glycol (PEG), oligomeric lactic acid (OLA), etc.) as well as essential oils and maleinized and/or epoxidized seed oils are widely used for flexible PLA film production. This article reviews the most relevant compostable PLA-plasticized flexible film formulations with an emphasis on plasticizer effect on the compostability rate of PLA polymeric matrix with the aim to get information of the possibility to use plasticized PLAbased formulatios as compostable films for sustainable industrial packaging production.</p>

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“…better stability, shorter generation time, easy to cultivate using inexpensive carbon and nitrogen sources, and larger amount of enzymes secreted (Li et al 2008). Additionally, several thermophilic Actinobacteria have been reported as cellulase producers, for example, Thermomonospora cellulolytica YIM 77510 T and T. amylolytica YIM 77502 T (Jiao et al 2015), Actinomadura keratinilytica T16-1 (Sukkhum et al 2011), and A. miaoliensis TF1 (Sriyapai et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

et al. 2019

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Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigated 17 thermophilic Actinobacteria isolated from the soil of geysers in the Cisolok geothermal area, West Java, as potential producers of cellulase. Screening for cellulase was performed on minimal (Mm) agar medium with and without the addition of 1% (w/v) carboxymethylcellulose (CMC) and microcrystalline cellulose (MCC), then incubated at 45, 50, 55 and 60°C for up to 7 days. Formation of clear zones around colonies indicated cellulose hydrolysis. The results showed that 15, 14, 4, and 3 isolates showed cellulolytic activity on CMC agar medium at 45, 50, 55, and 60°C, respectively, after 7 days of incubation. Three potential isolates showed cellulolytic activity on MCC agar medium after being incubated for 7 days at 45°C. Molecular identification based on the 16S rRNA gene was performed for three isolates with positive cellulolytic activity at 60°C. The results showed that the three isolates are closely related to Actinomadura keratinilytica WCC-2265T with 99.93-100% sequence similarities. A phylogenetic tree based on 16S rRNA gene sequences confirmed that the three isolates were clustered together with Actinomadura keratinilytica WCC-2265T with 100% bootstrap value. The tree also showed that cellulase producers and non-cellulase producers in Thermomonosporaceae are grouped into different clades.

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Isolation and Characterization of Polyester-Based Plastics-Degrading Bacteria from Compost Soils

Cited by 43 publications

References 31 publications

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Influence of plasticizers on the compostability of polylactic acid

Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

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