Isolation and Characterization of Pepsin-soluble Collagens from Bones, Skins, and Tendons in Duck Feet

Abstract

The objectives of this study were conducted to characterize pepsin-soluble collagen (PSC) extracted from bones (PSC-B), skins (PSC-S), and tendons (PSC-T) of duck feet and to determine their thermal and structural properties, for better practical application of each part of duck feet as a novel source for collagen. PSC was extracted from each part of duck feet by using 0.5 M acetic acid containing 5% (w/w) pepsin. Electrophoretic patterns showed that the ratio between α1 and α2 chains, which are subunit polypeptides forming collagen triple helix, was approximately 1:1 in all PSCs of duck feet. PSC-B had slightly higher molecular weights for α1 and α2 chains than PSC-S and PSC-T. From the results of differential scanning calorimetry (DSC), higher onset (beginning point of melting) and peak temperatures (maximum point of curve) were found at PSC-B compared to PSC-S and PSC-T (p<0.05). Fourier transform infrared spectroscopy (FT-IR) presented that PSC-S and PSC-T had similar intermolecular structures and chemical bonds, whereas PSC-B exhibited slight difference in amide A region. Irregular dense sheet-like films linked by random-coiled filaments were observed similarly. Our findings indicate that PSCs of duck feet might be characterized similarly as a mixture of collagen type I and II and suggest that duck feet could be used for collagen extraction without deboning and/or separation processes.

Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin

“…This stability is also affected by the body temperature of a given organism and the temperature of their habitat [24]. Interestingly, the melting temperature of PSC from red stingray skin was close to that of duck feet (92.48 °C), but higher than both duck skin (86.22 °C) and duck tendon (88.46 °C) [41]. Considering the importance of thermal stability in the application of collagen, we propose that PSC derived from red stingray skin is an excellent possible alternative to terrestrial sources of collagen.…”

Hydrolyzed Collagen—Sources and Applications

“…Alternative sources for native collagen extraction that are not of bovine or porcine origin have been developed from ovine tendon and skin [26,27]; fish tissue such as bones, skin, and scales or waste fish byproducts, or others sources such as chicken, duck, and rabbit skin [28,29,30,31,32].…”

Effects of Gelatin Hydrolysates Addition on Technological Properties and Lipid Oxidation of Cooked Sausage

“…Duck skin gelatin was prepared according to a previously described extraction procedure ( Kim et al, 2016 ; Tümerkan et al, 2019 ) with minor modifications. The frozen duck skin was thawed in a 4°C refrigerator for 24 h. The excessive subcutaneous fat was removed, and the duck skin was washed with tap water several times.…”