Isolation and Characterization of Partial Sequence of &lt;em&gt;merA&lt;/em&gt; Gene from Mercury Resistant Bacterium &lt;em&gt;Klebsiella pneumonia&lt;/em&gt; Isolated from Sario River Estuary Manado

Fatimawali, Fatimawali; Kepel, Billy J.; Yusuf, Irawan; Badaruddin, Fatmawaty; Natsir, Rosdiana; Retnoningrum, Debbie Soefie

doi:10.19026/rjees.6.5754

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…This finding was differ-ent from Fatimawali's merA study isolated from Klebsiella pneumoniae. The merA partial gene was successfully obtained 285 bp length, which encoded 94 aa due to using a single primer set (Fatimawali et al 2014). Using multiple primer sets was recommended to obtain a full sequence of merA genes.…”

Section: Discussionmentioning

confidence: 99%

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Putri

Rahayu

Khasanah³

et al. 2021

Indones. J. Biotechnol.

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Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX‐5x‐1 and pET‐28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury‐resistant Streptomyces and cloning the full‐length merA gene in Indonesia.

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Section: Discussionmentioning

confidence: 99%

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Putri

Rahayu

Khasanah³

et al. 2021

Indones. J. Biotechnol.

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“…It has no beneficial functions in living organisms, and this toxic compound can accumulate in the food chain ( Jan et al 2009 ). The mercury resistance system is encoded by the mer operon, which reduces Hg 2+ into elemental mercury via the mercuric reductase enzyme (MerA) ( Boyd and Barkay 2012 ; Fatimawali et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia

Miloud

Dziri

Ferjani³

et al. 2021

Polish Journal of Microbiology

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Environmental bacteria belonging to various families were isolated from polluted water collected from ten different sites in Tunisia. Sites were chosen near industrial and urban areas known for their high degree of pollution. The aim of this study was to investigate cross-resistance between heavy metals (HM), i.e., silver, mercury and copper (Ag, Hg, and Cu), and antibiotics. In an initial screening, 80 isolates were selected on ampicillin, and 39 isolates, retained for further analysis, could grow on a Tris-buffered mineral medium with gluconate as carbon source. Isolates were identified based on their 16S rRNA gene sequence. Results showed the prevalence of antibiotic resistance genes, especially all isolates harbored the blaTEM gene. Some of them (15.38%) harbored blaSHV. Moreover, several were even ESBLs and MBLs-producers, which can threaten the human health. On the other hand, 92.30%, 56.41%, and 51.28% of the isolates harbored the heavy metals resistance genes silE, cusA, and merA, respectively. These genes confer resistance to silver, copper, and mercury. A cross-resistance between antibiotics and heavy metals was detected in 97.43% of our isolates.

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Isolation and Characterization of Partial Sequence of <em>merA</em> Gene from Mercury Resistant Bacterium <em>Klebsiella pneumonia</em> Isolated from Sario River Estuary Manado

Cited by 2 publications

References 17 publications

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia

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