First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia

Miloud, Samar Ben; Dziri, Olfa; Ferjani, Sana; Ali, Muntasir; Mysara, Mohamed; Boutiba, Ilhem; Chouchani, Chedly

doi:10.33073/pjm-2021-012

Cited by 4 publications

(5 citation statements)

References 46 publications

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“…Walsh and Caslake (2016) showed a significant relationship between mercury and multi-antibiotic resistance in E. coli isolated from the aquatic environment. Another study reported that there is cross-resistance between various antibiotics and silver, copper, and mercury in bacteria isolated from polluted water (Miloud et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Celik,

Sipahi,

Kekec

et al. 2023

J Hellenic Vet Med Soc

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One of the biggest problems in the modern world is environmental pollution. All organisms are affected by the resulting pollution, albeit at different levels. One of the most important causes of pollution is the stable and non-perishable compounds formed due to heavy metals used in a wide range of environments. Heavy metal pollution, especially in the seas, causes cumulative accumulations in aquatic life, food and other products obtained from aquatic life, in living things that consume these products, and related diseases. The most critical sign of heavy metal pollution in water is the resistance formed against heavy metals in bacteria living in these waters. To determine the resistance to heavy metals in bacteria, the presence of resistance to Copper, Mercury and Manganese heavy metals in E. coli isolated from 18 mussels and 16 shrimps was genotypically investigated. For this purpose, the presence of pcoR genes for the determination of copper resistance, merA for the determination of mercury resistance, and mntR for the determination of manganese resistance was investigated in both plasmid and genomic DNA. As a result of the study, resistance genes were detected against heavy metals in 31 (91.17%) of 34 E. coli isolates examined. The presence of the pcoR gene (copper resistance) was found in 2 isolates (5.88%), the merA gene (mercury resistance) in only one isolate (2.94%) and the mntR gene (manganese resistance) in 8 isolates (23.52%). While pcoR and merA genes were not observed together in any isolates, pcoR and mntR genes were detected together in the genetic material of 10 isolates (29.41%). In comparison, mntR and merA genes were detected together in the genetic material of 7 isolates (20.58%). In 3 samples, all of the resistance genes against heavy metals were detected (8.82%).

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Section: Discussionmentioning

confidence: 99%

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Celik,

Sipahi,

Kekec

et al. 2023

J Hellenic Vet Med Soc

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“…The stability of QACs, azoles, chlorhexidine, and metals in the environment has led to concerns that these biocide classes may persist in wastewater facilities from hospitals and food processing plants, as well as in run-off from agriculture. Several studies detected low levels of biocidal chemicals in wastewater, food, soil, mines, and other environmental sources [ 77 , 78 , 110 , 117 , 119 ]. Researchers hypothesize that biocide-tolerant bacteria rising from low concentration exposure in these niche environments may result in biocide-mediated antibiotic cross-resistance development in the real world.…”

Section: Discussionmentioning

confidence: 99%

“…The overexpression of efflux pump proteins and a reduced expression of porins have been described as possible mechanisms of metal tolerance [ 74 , 75 ], as well as an enzymatic reduction of the cation to the metal [ 60 , 70 ]. Generally, authors have reached the agreement that the exact mechanisms still remain unclear and are also organism specific [ 76 , 77 , 78 , 79 ]. Therefore, further investigation is needed.…”

Section: Biocides—major Classes Chemistries Modes Of Action and Resis...mentioning

confidence: 99%

“…These isolates showed metal tolerance to copper and antibiotic resistance to ampicillin and chloramphenicol [ 78 ]. Cross-resistance to ampicillin has also been described by Miloud et al, who isolated environmental species selected for ampicillin and observed resistance with other antibiotics in addition to silver and copper tolerance [ 77 ].…”

Section: Summary Of Studies Investigating the Potential For Antibioti...mentioning

confidence: 99%

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Current Understanding of Potential Linkages between Biocide Tolerance and Antibiotic Cross-Resistance

et al. 2023

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Antimicrobials (e.g., antibiotics and biocides) are invaluable chemicals used to control microbes in numerous contexts. Because of the simultaneous use of antibiotics and biocides, questions have arisen as to whether environments commonly treated with biocides (e.g., hospitals, food processing, wastewater, agriculture, etc.) could act as a reservoir for the development of antibiotic cross-resistance. Theoretically, cross-resistance could occur if the mechanism of bacterial tolerance to biocides also resulted in antibiotic resistance. On the other hand, biocides would likely present a higher evolutionary barrier to the development of resistance given the different modes of action between biocides and antibiotics and the broad-based physicochemical effects associated with most biocides. Published studies have shown that the induction of biocide tolerance in a laboratory can result in cross-resistance to some antibiotics, most commonly hypothesized to be due to efflux pump upregulation. However, testing of environmental isolates for biocide tolerance and antibiotic cross-resistance has yielded conflicting results, potentially due to the lack of standardized testing. In this review, we aim to describe the state of the science on the potential linkage between biocide tolerance and antibiotic cross-resistance. Questions still remain about whether the directed evolution of biocide tolerance and the associated antibiotic cross-resistance in a laboratory are or are not representative of real-world settings. Thus, research should continue to generate informative data to guide policies and preserve these tools’ utility and availability.

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“…And the antibiotics suspension (0.3 ml) was transferred to the well. The plates were then kept at 37°C for a further 24 hours [22], [23].…”

Section: Determination Of Antibiotic Susceptibilitymentioning

confidence: 99%

In Vitro and In Silico Analysis of the Gut Microbiome of Zebrafish for Bioremediation Approach of Zinc-Contaminated Aquatic Environments

2023

JPTCP

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The discovery of bacterial organisms that aid in heavy metal bioremediation opens up new possibilities for removing radioactive compounds and heavy metals from polluted water sources. Accordingly, this study aims to the identification of Zinc resistant bacteria from the gut microbiome of zebrafish and to evaluate its bioremediation capability. The isolates, namely FG01 (Enterobacter cloacae), FG02 (Citrobacter freundii), and FG03 (Aeromonas hydrophila) identified based on 16S rRNA gene sequencing resistant to Zinc and antibiotics like Ampicillin and Amoxicillin and also selected for bioaccumulation studies. The MTC values of the three zinc-resistant bacteria from the Zebrafish gut were evaluated, and the results revealed that the growth of the isolate FG02 was better than the others, while the growth of FGO3 and FG01 decreased at the concentrations of 10 ppm and 15 ppm. By docking studies, the Zinc binding proteins (ZBPs) were discovered and beta-lactamase showed the best binding affinity compared to the other protein. In the process of treating wastewater, zinc-resistant bacteria from the gut microbiome of zebrafish are normally present and can demonstrate their capacity to adsorb heavy metals. The ZBP can be used later as a novel absorbent for heavy metal removal technology.

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First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia

Cited by 4 publications

References 46 publications

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Current Understanding of Potential Linkages between Biocide Tolerance and Antibiotic Cross-Resistance

In Vitro and In Silico Analysis of the Gut Microbiome of Zebrafish for Bioremediation Approach of Zinc-Contaminated Aquatic Environments

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