Isolation and characterization of novel microsatellite markers from the leafhopper Hishimonus phycitis distant (Hemiptera: Cicadellidae)

Abstract

The leafhopper Hishimonus phycitis has received considerable attention as a vector of witches' broom disease of lime phytoplasma. In the present study, nine polymorphic microsatellite loci were isolated from a repeat-enriched genomic library of H. phycitis. The number of alleles varied between 2 and 4 with an average of 2.80 per locus. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.28 to 0.80, respectively. The Polymorphism Information Content varied from 0.25 to 0.75 with an average of 0.55. These microsatellites will be useful for future genetic studies of H. phycitis populations and could help in the development of an efficient control strategy against this vector.

Microsatellite markers from tea green leafhopper Empoasca (Matsumurasca) onukii: a powerful tool for studying genetic structure in tea plantations

“…Although previous studies reported 27 microsatellite markers for three different leafhopper species [ 16 , 17 , 20 ], our attempts to amplify these markers in E. ( M. ) onukii populations failed. This is not surprising, given that two of the three species in these previous studies belong to the distantly related leafhopper subfamily Deltocephalinae and that leafhoppers, in general, are a phyletically diverse and widespread group of insects.…”

“…Papura et al isolated ten and eight microsatellite markers, respectively, for Scaphoideus titanus Ball and Empoasca vitis (Goëthe), and subsequently showed that European S. titanus populations originated from northeastern North America [ 16 – 18 ]. Shabani et al suggested that climatic and/or geogeraphical barriers might induce population genetic differentiation of the leafhopper Hishimous phycitis using the mitochondrial cytochrome oxidase I ( COI ) gene and nine microsatellite DNA markers isolated by FIASCO [ 19 , 20 ]. Unfortunately, two of the three leafhopper species included in these previous studies are distantly related to Empoasca , belonging to a separate cicadellid subfamily (Deltocephalinae), and we were unable to consistently amplify the microsatellite markers developed for these species in Chinese E. ( M. ) onukii populations.…”

Contribution to the knowledge of selected genera of the tribe Opsiini (Hemiptera: Cicadellidae: Deltocephalinae) from the Kingdom of Saudi Arabia

“…Hishimonus currently includes more than 50 species known from the Oriental, Palaearctic, Afrotropical and Australasian Regions (ZAHNISER 2018, FLETCHER & DAI 2018. Hishimonus phycitis (Distant, 1908) has been reported as a vector of the Witches' broom disease of lime (WBDL) (ZREIK et al 1995, SHABANI et al 2011. WBDL is associated with 'Candidatus Phytoplasma aurantifolia', considered one of the most lethal plant pathogens , SHABANI et al 2013.…”

“…Economic importance. The genus Hishimonus is known as a vector of Witches' broom disease of lime (WBDL) considered one of the most lethal plant pathogens and widely distributed in the Arabian Peninsula(SHABANI et al 2011(SHABANI et al , 2013AL-SALEH & AMER 2014).…”

Generation and Expression in Plants of a Single-Chain Variable Fragment Antibody Against the Immunodominant Membrane Protein of Candidatus Phytoplasma Aurantifolia

“…The symptoms include small chlorotic leaves, rapidly proliferating shoots, and short internodes, causing the plant to die within 3-4 years. Witches' broom of lime is caused by Candidatus Phytoplasma aurantifolia, which is naturally transmitted by the leafhopper Hishimonus phycitis Distant [26]. Phytoplasmas are wall-less, phloem-restricted bacterial pathogens of the class Mollicutes that persistently colonize their plant hosts [31].…”