Isolation and characterization of novel tachykinins from the posterior salivary gland of the common octopus Octopus vulgaris

Kanda, Atsuhiro; Ukena, Kazuyoshi; Takuwa‐Kuroda, Kyoko; Minakata, Hiroyuki

doi:10.1016/s0196-9781(02)00274-7

Cited by 56 publications

(52 citation statements)

References 21 publications

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“…Contractile Activities of Ci-TKs on Gut Tissues-TKs are known to induce a characteristic contraction of gut tissues (1,7,19,20). Nevertheless, Ci-TKs had no contractile effect on the Ciona gut (data not shown), most likely due to the absence of intestinal muscular tissues in the ascidian gut (29).…”

Section: Identification Of the Ci-tk Peptides Cdna And Genomicmentioning

confidence: 91%

“…For the bioassay, the guinea pig ileum was a gift from Dr. Toshiaki Fujii. The contractile effects of Ci-TKs and other TKs were observed as described previously (19).…”

Section: Functional Analysis Of Ci-tk-r Expressed In Xenopusmentioning

confidence: 99%

“…In protostomes, two types of TK-like peptides, invertebrate TK and TK-related peptides (TKRPs), have so far been identified. Peptides of the former group, containing the identical C-terminal TK consensus motif, are expressed exclusively in the salivary gland and are devoid of any activity on the cognate tissues, indicating that the TK-like peptides are not functional counterparts of vertebrate TKs (9,19,20). TKRPs exert a TK-like contractile activity, and the expression of the TKRP gene is observed in the central nervous system (20).…”

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confidence: 99%

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Tachykinin and Tachykinin Receptor of an Ascidian, Ciona intestinalis

Satake

Ogasawara

Kawada

et al. 2004

Journal of Biological Chemistry

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Tachykinins (TKs) are the most prevalent vertebrate brain/gut peptides. In this study, we originally identified authentic TKs and their receptor from a protochordate, Ciona intestinalis. The Ciona TK (Ci-TK) precursor, like mammalian ␥-preprotachykinin A (␥-PPTA), encodes two TKs, Ci-TK-I and -II, including the -FXGLM-NH 2 vertebrate TK consensus. Mass spectrometry of the neural extract revealed the production of both Ci-TKs. Ci-TK-I contains several Substance P (SP)-typical amino acids, whereas a Thr is exceptionally located at position 4 from the C terminus of Ci-TK-II. The Ci-TK gene encodes both Ci-TKs in the same exon, indicating no alternative generation of Ci-TKs, unlike the PPTA gene. These results suggested that the alternative splicing of the PPTA gene was established during evolution of vertebrates. The only Ci-TK receptor, Ci-TK-R, was equivalently activated by Ci-TK-I, SP, and neurokinin A at physiological concentrations, whereas Ci-TK-II showed 100-fold less potent activity, indicating that the ligand selectivity of Ci-TK-R is distinct from those of vertebrate TK receptors. Ci-TK-I, like SP, also elicited the typical contraction on the guinea pig ileum. The Ci-TK gene was expressed in neurons of the brain ganglion, small cells in the intestine, and the zone 7 in the endostyle, which corresponds to the vertebrate thyroid gland. Furthermore, the Ci-TK-R mRNA was distributed in these three tissues plus the gonad. These results showed that Ci-TKs play major roles in sexual behavior and feeding in protochordates as brain/gut peptides and endocrine/paracrine molecules. Taken together, our data revealed the biochemical and structural origins of vertebrate TKs and their receptors.

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Section: Identification Of the Ci-tk Peptides Cdna And Genomicmentioning

confidence: 91%

“…For the bioassay, the guinea pig ileum was a gift from Dr. Toshiaki Fujii. The contractile effects of Ci-TKs and other TKs were observed as described previously (19).…”

Section: Functional Analysis Of Ci-tk-r Expressed In Xenopusmentioning

confidence: 99%

mentioning

confidence: 99%

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Tachykinin and Tachykinin Receptor of an Ascidian, Ciona intestinalis

Satake

Ogasawara

Kawada

et al. 2004

Journal of Biological Chemistry

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“…These factors thus suggest that mollusks may have developed defense strategies to protect themselves from exposure to a high density of microorganisms, including potential pathogens. The use of secondary metabolites is well documented in the phylum Mollusca as part of their communication systems (22)(23)(24), predatory behavior (25,26), and defensive secretions (27)(28)(29)(30). The abundance, diversity, and chemical potential of mollusks make this phylum of interest for studies of microbial symbiosis.…”

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confidence: 99%

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Davis

Fricke

Hamann

et al. 2013

Appl Environ Microbiol

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dSacoglossans are characterized by the ability to sequester functional chloroplasts from their algal diet through a process called kleptoplasty, enabling them to photosynthesize. The bacterial diversity associated with sacoglossans is not well understood. In this study, we coupled traditional cultivation-based methods with 454 pyrosequencing to examine the bacterial communities of the chemically defended Hawaiian sacoglossan Elysia rufescens and its secreted mucus. E. rufescens contains a defense molecule, kahalalide F, that is possibly of bacterial origin and is of interest because of its antifungal and anticancer properties. Our results showed that there is a diverse bacterial assemblage associated with E. rufescens and its mucus, with secreted mucus harboring higher bacterial richness than entire-E. rufescens samples. The most-abundant bacterial groups affiliated with E. rufescens and its mucus are Mycoplasma spp. and Vibrio spp., respectively. Our analyses revealed that the Vibrio spp. that were highly represented in the cultivable assemblage were also abundant in the culture-independent community. Epifluorescence microscopy and matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) were utilized to detect the chemical defense molecule kahalalide F on a longitudinal section of the sacoglossan.

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“…Experimentally, the PSG extract of Octopus aegina showed anticancer activity in vitro and in vivo cancer models (Karthigayan et al, 2006). Generally, the chemical components of PSG extract contain amines, proteins, and peptides (Kanda et al, 2003). These compounds include the endecapeptideeledoisin and octopamine (Erspamer, 1984), 5-hydroxytryptamine (Bacq, 1951), amine substances (Hartman et al, 1960).…”

Section: Introductionmentioning

confidence: 99%

Comparative histopathological and histochemical impacts induced by the posterior salivary gland and ink sac extracts of Octopus vulgaris in mice

Moustafa

Awaad

2016

The Journal of Basic & Applied Zoology

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Octopus vulgaris is of great importance for humans as a food resource and in fishery. The present study gave the first new insight about the comparative histopathological and histochemical impacts of oral administration of a single dose (25 mg/kg) of the posterior salivary gland (PSG) and ink sac (IS) extracts of O. vulgaris in mice. Histopathologically, the changes in the liver of PSG and IS extracts administrated mice were lymphocyte aggregations, cytoplasmic vacuolar degeneration and enlargement and congestion of hepatic portal veins. The changes caused by the PSG extract were more than those of the IS extract. In the treated spleen, IS extract showed higher impacts more than the PSG extract and occurred as lymphocyte aggregations, red pulp infiltration, increase of megakaryocytes number and mild distortion in the follicle shape. PSG and IS extracts exerted similar impacts in the ileum which detected as villi hyperplasia, epithelial and lamina propria degeneration, epithelial detachment and lymphocytes infiltration. Histochemically, IS extract increased hepatic collagenous fibers more than PSG extract especially after 7 days of administration. Both extracts reduced the liver general carbohydrates and illeal mucopolysaccharide biosynthesis. PSG and IS extracts exerted significant elevation in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzymes. Hematological studies after PSG administration revealed a significant increase in leukocytic count, and platelets and a significant decrease in lymphocytes and granulocytes. However, IS extract caused a significant increase in platelets. The present data could introduce new information about the toxicity of these extracts especially in the countries which depend on O. vulgaris ink in their food.

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Isolation and characterization of novel tachykinins from the posterior salivary gland of the common octopus Octopus vulgaris

Cited by 56 publications

References 21 publications

Tachykinin and Tachykinin Receptor of an Ascidian, Ciona intestinalis

Tachykinin and Tachykinin Receptor of an Ascidian, Ciona intestinalis

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Comparative histopathological and histochemical impacts induced by the posterior salivary gland and ink sac extracts of Octopus vulgaris in mice

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