Isolation and Characterization of Novel Denitrifying Bacterium &amp;lt;italic&amp;gt;Geobacillus&amp;lt;/italic&amp;gt; sp. SG-01 Strain from Wood Chips Composted with Swine Manure

Yang, Seung-Hak; Cho, Jin-Kook; Lee, Soon-Youl; Abanto, Oliver D.; Kim, Soo‐Ki; Ghosh, Chiranjit; Lim, Joung-Soo; Hwang, Seong Gu

doi:10.5713/ajas.2013.13184

Cited by 14 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Examination of carbohydrate degrading activity of the isolate demonstrated that the isolate could be considered as possible source of biotechnologically valuable enzymes such as cellulases, amylase and xylanase. G. thermodenitrificans SG-01, SG-02 and SG-03 gave positive result for utilization of fructose, fructose and sucrose and sucrose, respectively (Yang et al 2013).…”

Section: Resultsmentioning

confidence: 97%

“…Geobacillus sp. SG 01 presented growth at NaCl concentration (0-2%) with optimal growth at 1% NaCl (Yang et al 2013). Geobacillus pallidus p26 grew within the NaCl range of 2-5% (Celik et al 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Geobacillus sp. SG 01 displayed optimum growth at pH 7.0 while no growth was observed at pH values higher than 9.5 (Yang et al 2013 ). Geobacillus pallidus p26 isolated from Pasinler hot spring, Erzurum, Turkey grew optimally at pH 7.5 (Celik et al 2014).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan

Zahoor¹,

Javed²,

Babarl³

2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

A thermophilic bacterium (TP-2) was isolated from the Tatta Pani hot spring in Azad Kashmir and was characterized using phenotypic and genotypic characters. The strain developed cream colored, round, smooth, flat and slimy colonies while the cells were Gram positive rods that ranged in size from about 2.1-3.6 μm to 0.2-0.3 μm in width. Sequence analysis of its 16S rRNA gene showed that isolate TP-2 had 89% homology with Geobacillus debilis. It grew within pH range of 5.5 to 8.5 with optimum growth at pH 7.0. The isolate showed optimum growth at 65ºC and gave positive results for gelatin hydrolysis (GEL), ortho nitrophenyl-β-D-galactopyranosidase (ONPG), and nitrate production and produced acid from sucrose, glucose and maltose. It utilized glucose, fructose, maltose, lactose, sucrose, xylan, starch, filter paper and carboxymethylcellulose as sole carbon source. Isolate TP-2 produced significant amount of industrially important enzymes i.e. extracellular α-amylase, CMCase, FPase, Xylanase, Protease and Lipase and intracellular CMCase and FPase.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan

Zahoor¹,

Javed²,

Babarl³

2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Most denitrifying bacteria are suitable for growth in neutral and weak alkali environments (Zhang et al 2012; Ye et al 2016). The optimum pH of denitrification by most denitrifying bacteria was 7–8 (Tang et al 2014; He, Li, and Xu 2015; Yang et al 2013). However, when the pH was out of the optimal range, the denitrification capacity can be decreased.…”

Section: Discussionmentioning

confidence: 99%

Identification and denitrification characteristics of a salt-tolerant denitrifying bacterium Pannonibacter phragmitetus F1

et al. 2019

View full text Add to dashboard Cite

A salt-tolerant denitrifying bacterium F1 was isolated in this study, which has high nitrite (NO2−–N) and nitrate (NO3−–N) removal abilities. The salt tolerance capacity of strain F1 was further verified and the effects of initial pH, initial NaNO2 concentration and inoculation size on the denitrification capacity of strain F1 under saline conditions were evaluated. Strain F1 was identified as Pannonibacter phragmitetus and named Pannonibacter phragmitetus F1. This strain can tolerate NaCl concentrations up to 70 g/L, and its most efficient denitrification capacity was observed at NaCl concentrations of 0–10 g/L. Under non-saline condition, the removal percentages of NO2−–N and NO3−–N by strain Pannonibacter phragmitetus F1 at pH of 10 and inoculation size of 5% were 100% and 83%, respectively, after cultivation for 5 days. Gas generation was observed during the cultivation, indicating that an efficient denitrification performance was achieved. When pH was 10 and the inoculation size was 5%, both the highest removal percentages of NO2−–N (99%) and NO3−–N (95%) by strain Pannonibacter phragmitetus F1 were observed at NaCl concentration of 10 g/L. When the NaCl concentration was 10 g/L, strain Pannonibacter phragmitetus F1 can adapt to a wide range of neutral and alkaline environments (pH of 7–10) and is highly tolerant of NaNO2 concentration (0.4–1.6 g/L). In conclusion, strain Pannonibacter phragmitetus F1 has a great potential to be applied in the treatment of saline wastewater containing high nitrogen concentrations, e.g. coastal aquaculture wastewater.

show abstract

“…They are usually sensitive to oxygen, and the expression of enzymes related to the denitrification process might be suppressed under aerobic conditions (Zheng et al, 2012; Zhou et al, 2014; Zhao et al, 2018). Aerobic denitrifying bacterium is able to utilize oxygen and nitrate or nitrite as electron acceptors simultaneously under aerobic condition (Yang et al, 2013; Huang et al, 2015; Zhao et al, 2018). Thus, compared to conventional denitrifying bacterium, aerobic denitrifying bacterium might be more suitable for nitrite pollution elimination in scenarios including intensive aquaculture industry where adequate dissolved oxygen was needed for aquatic animals (Li et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A Novel Nitrite-Base Aerobic Denitrifying Bacterium Acinetobacter sp. YT03 and Its Transcriptome Analysis

Xiao

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Nitrite in a water environment is very harmful to humans and aquatic animals. A novel aerobic denitrifying bacterium able to utilize NO2--N as the only nitrogen source was isolated for the purpose of removing nitrite from water, which was identified as Acinetobacter sp. and named as YT03. The growth and denitrification activity of strain YT03 was assessed comprehensively. Results showed that the nitrite in water with an initial concentration of 10 mg L–1 could be completely removed within 6 h by strain YT03, and the optimal conditions for strain YT03 to remove nitrite were as follows: sodium succinate as the carbon source, C/N ratio of 16, pH of 6.5, temperature of 30°C, and shaking speed of 250 rpm. An RNA-Seq transcriptome analysis was used to find genes associated with nitrite removal. Compared with the removal of ammonia nitrogen, 47 genes were significantly differentially expressed, including 20 up-regulated and 27 down-regulated genes, mainly involved in the transport process, biosynthetic process, and so on. And among the differentially expressed genes, C4-dicarboxylate transporter (DctA) and nitrate/nitrite transporter (Nrt) might be of importance for the efficient utilization of carbon and nitrogen sources in aerobic nitrite denitrification with sodium succinate by strain YT03.

show abstract

Isolation and Characterization of Novel Denitrifying Bacterium <italic>Geobacillus</italic> sp. SG-01 Strain from Wood Chips Composted with Swine Manure

Cited by 14 publications

References 36 publications

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan

Identification and denitrification characteristics of a salt-tolerant denitrifying bacterium Pannonibacter phragmitetus F1

A Novel Nitrite-Base Aerobic Denitrifying Bacterium Acinetobacter sp. YT03 and Its Transcriptome Analysis

Contact Info

Product

Resources

About