Identification and denitrification characteristics of a salt-tolerant denitrifying bacterium Pannonibacter phragmitetus F1

Abstract

A salt-tolerant denitrifying bacterium F1 was isolated in this study, which has high nitrite (NO2−–N) and nitrate (NO3−–N) removal abilities. The salt tolerance capacity of strain F1 was further verified and the effects of initial pH, initial NaNO2 concentration and inoculation size on the denitrification capacity of strain F1 under saline conditions were evaluated. Strain F1 was identified as Pannonibacter phragmitetus and named Pannonibacter phragmitetus F1. This strain can tolerate NaCl concentrations up to 70 g/L, and its most efficient denitrification capacity was observed at NaCl concentrations of 0–10 g/L. Under non-saline condition, the removal percentages of NO2−–N and NO3−–N by strain Pannonibacter phragmitetus F1 at pH of 10 and inoculation size of 5% were 100% and 83%, respectively, after cultivation for 5 days. Gas generation was observed during the cultivation, indicating that an efficient denitrification performance was achieved. When pH was 10 and the inoculation size was 5%, both the highest removal percentages of NO2−–N (99%) and NO3−–N (95%) by strain Pannonibacter phragmitetus F1 were observed at NaCl concentration of 10 g/L. When the NaCl concentration was 10 g/L, strain Pannonibacter phragmitetus F1 can adapt to a wide range of neutral and alkaline environments (pH of 7–10) and is highly tolerant of NaNO2 concentration (0.4–1.6 g/L). In conclusion, strain Pannonibacter phragmitetus F1 has a great potential to be applied in the treatment of saline wastewater containing high nitrogen concentrations, e.g. coastal aquaculture wastewater.

Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification

“…In addition, W30 exhibited a series of high NO 3 − -N removal efficiencies from neutral to alkaline pHs. The result is consistent with that of known HN-AD microbes such as Pannonibacter phragmitetus F1 [46] and a strain of Pannonibacter isolated from a Hungarian soda lake [47]. It is believed that acidic pH may affect free ammonia and ammonia monooxygenase in the environment, although explicit mechanisms need to be further explored [38].…”

Simultaneous Nitrogen Removal and Plant Growth Promotion Using Salt-‍tolerant Denitrifying Bacteria in Agricultural Wastewater

“…The salinity levels of coastal aquaculture wastewater range between 2 and 35‍ ‍g L –1 NaCl ( Li et al , 2018 ). The salt-tolerant bacteria Pannonibacter phragmitetus F1 ( Wang et al , 2019 ), Pseudomonas sp. MSD4 ( Zeng et al , 2020 ), and Bacillus sp.…”

“…Moreover, it removed 62.4% of TN (100‍ ‍mg L –1 ) at 7% salinity and pH 7.0 and 72.2% of TN at 3% salinity and pH 9.0 ( Zeng et al , 2020 ). When cultured under 30 and 70‍ ‍g L –1 NaCl conditions, Panagrobacterium phragneum F1 removed 23 and 36% of N ( Wang et al , 2019 ). Bacillus sp.…”

Hemodialysis catheter-related infection caused by Pannonibacter phragmitetus: a rare case report in China

“…Pannonibacter phragmitetus ( P. phragmitetus ) is a gram-negative rod, which is recognized from a Hungarian soda lake in 2003 ( Borsodi et al., 2003 ). P. phragmitetus is commonly applied to environmental pollution control due to its ability to remove nitrogen and metals in water and soil ( Bai et al., 2019 ; Chai et al., 2019 ; Wang et al., 2019 ; Liao et al., 2020 ; Saravanan et al., 2021 ). Although it was first identified as Achromobacter groups B and E from human blood ( Holmes et al., 1990 ; Holmes et al., 1993 ; Holmes et al., 2006 ), little is known about its pathogenicity.…”