Isolation and characterization of new efficient and competitive bean (Phaseolus vulgaris L.) rhizobia from Brazil

Hungría, Mariangela; Andrade, Diva Souza; Chueire, L. M. de O.; Probanza, A.; Guttierrez-Mañero, Francisco J.; Megı́as, Manuel

doi:10.1016/s0038-0717(00)00063-8

Cited by 190 publications

(135 citation statements)

References 33 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…These results differed from those found by Hungria et al (2000), which observed that, in Brazil, a mixture of these commercial strains caused a positive response to inoculation.…”

Section: Af3648391contrasting

confidence: 99%

See 1 more Smart Citation

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

-This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fi xation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the fi rst experiment, the nodulation and nitrogen fi xation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes fi lled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots fi lled with soil. The native soil population was identifi ed as Rhizobium sp. and was ineffi cient in nitrogen fi xation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fi xation capacity and exhibit differential responses when mixed to PRF-81.Index terms: Phaseolus vulgaris, biological nitrogen fi xation, intergenic space, phylogeny, sequencing, soil rhizobia population. Caracterização genética e capacidade de fi xação de nitrogênio de estirpes de Rhizobium em feijoeiroResumo -O objetivo deste trabalho foi a caracterização genética de quatro novas estirpes de Rhizobium e a avaliação de sua capacidade de fi xação de N 2 e nodulação, comparadas a estirpes comerciais e à população nativa de rizóbios de um Latossolo Vermelho. Dois experimentos foram conduzidos em blocos ao acaso, em casa de vegetação. No primeiro experimento, conduzido em tubetes com vermiculita, avaliaram-se a nodulação e a capacidade de fi xação das novas estirpes, em comparação com as estirpes comerciais CIAT-899 e PRF-81 e com a população nativa do solo. Das colônias puras isoladas, extraiu-se o DNA genômico e realizou-se o seqüenciamento do espaço intergênico, para a caracterização genética das estirpes e da população nativa de rizóbios. O segundo experimento foi realizado em vasos com solo, para determinação da produtividade e da nodulação do feijoeiro, cultivar Pérola, com o uso das estirpes isoladamente ou em mistura com a PRF-81. A população nativa do solo foi identifi cada como Rhizobium sp. e se mostrou inefi ciente na fi xação de nitrogênio. Foram encontradas três espécies de Rhizobium entre as quatro novas estirpes. As estirpes LBMP-4BR e LBMP-12BR estão entre as que têm maior capacidade de nodulação e fi xação de N 2 , e apresentam respostas diferenciadas quando misturadas à PRF-81.Termos para indexação: Phaseolus vulgaris, fi xação biológica de nitrogênio, espaço intergênico, fi logenia, seqüenciamento, população nativa de rizóbios.

show abstract

“…These results differed from those found by Hungria et al (2000), which observed that, in Brazil, a mixture of these commercial strains caused a positive response to inoculation.…”

Section: Af3648391contrasting

confidence: 99%

“…Since June 1998, two Rhizobium tropici strains -SEMIA 4077 (CIAT-899) and SEMIA 4080 (PRF-81) -have their use recommended, in mixture, for inoculation on common bean crops, in Brazil (Hungria et al, 2000). Nevertheless, further research to isolate new strains with genetic diversity and nitrogen fi xation effi ciency has been carried out.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…Nevertheless, high morphological diversity was observed among the obtained isolates, whereas the isolates showed unusual characteristics for the genus Rhizobium, such as neutral pH, slow growth and small production of mucus (Figure 1). High morphological diversity among the common bean rhizobia isolates has been reported in Brazil (HUNGRIA et al 2000;MOSTASSO et al 2002;OLIVEIRA et al 2011), reinforcing reports about the promiscuity of the common bean to establish association with the rhizobia soil community (OLIVEIRA et al 2011). …”

Section: Morphological Characterization Of Rhizobia Isolatessupporting

confidence: 54%

Morphophysiological characterization of rhizobia isolated from wild genotypes of the common bean

Sampaio¹,

Knupp²,

Fernandes³

et al. 2016

Biosci. J.

View full text Add to dashboard Cite

ABSTRACT:This study aimed to characterize, based on the morphology and on the use of carbon sources, rhizobial isolates obtained from nodules of wild common bean genotypes cultivated on soil samples from the states of Goiás, Minas Gerais and Paraná. Based on morphological and carbon source use, similarity matrixes were generated to perform clustering analysis based on the Jaccard index. A total of 523 isolates were obtained with the predominance of isolates that acidify the culture medium and showed fast growth. Isolates from the soils of Araucária and Prudentópolis produced a greater amount of mucus than those from Jussara, Nova Veneza, Uberlândia and Unaí soils. The rhizobia isolates obtained from soils of Goiás and Minas Gerais showed greater phenotypic diversity than those from the soils of Paraná. Analysis based on the use of different carbon sources revealed that rhizobia isolates obtained from soils of Goiás have higher metabolic diversity. Overall, the most part of isolates grouped with the reference strains, especially with the Rhizobium tropici and R. freirei strains used as inoculants for the common bean.

show abstract

“…However, most of the symbiotic interactions are not effective (13). Studies performed in this last decade have shown that the selection of effective strains adapted to local environmental conditions may represent a successful approach that should be pursued (8). Nevertheless, it is also very important to assess the genetic diversity of indigenous rhizobial communities within the main producing areas, as the composition of the local community can affect the response to inoculation with superior strains.…”

mentioning

confidence: 99%

Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

Cursino

Muro-Abad²,

Gomes³

et al. 2009

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

Isolation and characterization of new efficient and competitive bean (Phaseolus vulgaris L.) rhizobia from Brazil

Cited by 190 publications

References 33 publications

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Morphophysiological characterization of rhizobia isolated from wild genotypes of the common bean

Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

Contact Info

Product

Resources

About