Isolation and characterization of new compounds from seeds of Nigella sativa

Mehta, B. K.; Sharma, Uma; Agrawal, Sumit; Pandit, V.; Joshi, Nidhi; Gupta, Monika

doi:10.1007/s00044-007-9080-1

Cited by 16 publications

(12 citation statements)

References 25 publications

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“…It has been shown that the biological properties of the essential oils are directly associated with the chemical composition, which varies depending on the origin (Bourgou et al, 2010). Several of the pharmacologically interesting substances have also been isolated from Nigella seeds including isoquinoline and indazole alkaloids (Atta, 2003;Atta-ur-Rahman et al, 1995), saponins (Mehta et al, 2009), cycloartenols (Mehta et al, 2008), and flavonoid glycosides (Merfort et al, 1997).…”

Section: Introductionmentioning

confidence: 98%

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

Maulidiani

Sheikh

Mediani

et al. 2015

Phytochemistry Letters

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Section: Introductionmentioning

confidence: 98%

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

Maulidiani

Sheikh

Mediani

et al. 2015

Phytochemistry Letters

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“…While N. sativa and R. rosea extracts did not inhibit IBV, many of their phytochemicals are thought to be antiviral. For example, N. sativa seed extracts predominantly contain saponins, glycosides, terpenoids and alkaloids [38,[63][64][65][66][67], many of which are similar to known antiviral chemicals [38][39][40]68]. On the other hand, R. rosea root extracts consist of many kaempferol, herbacetin, dihydromyricetin, and myricetin derivatives [32].…”

Section: Discussionmentioning

confidence: 99%

Sambucus nigra extracts inhibit infectious bronchitis virus at an early point during replication

et al. 2014

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BackgroundInfectious bronchitis virus (IBV) is a pathogenic chicken coronavirus. Currently, vaccination against IBV is only partially protective; therefore, better preventions and treatments are needed. Plants produce antimicrobial secondary compounds, which may be a source for novel anti-viral drugs. Non-cytotoxic, crude ethanol extracts of Rhodiola rosea roots, Nigella sativa seeds, and Sambucus nigra fruit were tested for anti-IBV activity, since these safe, widely used plant tissues contain polyphenol derivatives that inhibit other viruses.ResultsDose–response cytotoxicity curves on Vero cells using trypan blue staining determined the highest non-cytotoxic concentrations of each plant extract. To screen for IBV inhibition, cells and virus were pretreated with extracts, followed by infection in the presence of extract. Viral cytopathic effect was assessed visually following an additional 24 h incubation with extract. Cells and supernatants were harvested separately and virus titers were quantified by plaque assay. Variations of this screening protocol determined the effects of a number of shortened S. nigra extract treatments. Finally, S. nigra extract-treated virions were visualized by transmission electron microscopy with negative staining.Virus titers from infected cells treated with R. rosea and N. sativa extracts were not substantially different from infected cells treated with solvent alone. However, treatment with S. nigra extracts reduced virus titers by four orders of magnitude at a multiplicity of infection (MOI) of 1 in a dose-responsive manner. Infection at a low MOI reduced viral titers by six orders of magnitude and pretreatment of virus was necessary, but not sufficient, for full virus inhibition. Electron microscopy of virions treated with S. nigra extract showed compromised envelopes and the presence of membrane vesicles, which suggested a mechanism of action.ConclusionsThese results demonstrate that S. nigra extract can inhibit IBV at an early point in infection, probably by rendering the virus non-infectious. They also suggest that future studies using S. nigra extract to treat or prevent IBV or other coronaviruses are warranted.

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“…For example, N. sativa seed extracts predominantly contain saponins, glycosides, terpenoids and alkaloids [38,[63][64][65][66][67], many of which are similar to known antiviral chemicals [38][39][40]68]. On the other hand, R. rosea root extracts consist of many kaempferol, herbacetin, dihydromyricetin, and myricetin derivatives [32].…”

Section: Discussionmentioning

confidence: 99%

“…Studies of murine cytomegalovirus infection and hepatitis C infection lend support to the plant's antiviral potential in vivo, as well [36,37]. Additionally, N. sativa compound extracts, especially its saponins, alkaloids, and flavonols, show similarities with known antiviral chemicals [38][39][40]. Finally, S. nigra extract has successfully inhibited influenza A and B viruses in vitro and in vivo [41].…”

Section: Introductionmentioning

confidence: 91%

Sambucus nigra Extracts Inhibit Infectious Bronchitis Virus at an Early Point during Replication

Chen¹,

Zuckerman²,

Brantley³

et al. 2013

Planta Med

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Background: Infectious bronchitis virus (IBV) is a pathogenic chicken coronavirus. Currently, vaccination against IBV is only partially protective; therefore, better preventions and treatments are needed. Plants produce antimicrobial secondary compounds, which may be a source for novel anti-viral drugs. Non-cytotoxic, crude ethanol extracts of Rhodiola rosea roots, Nigella sativa seeds, and Sambucus nigra fruit were tested for anti-IBV activity, since these safe, widely used plant tissues contain polyphenol derivatives that inhibit other viruses.

show abstract

Isolation and characterization of new compounds from seeds of Nigella sativa

Cited by 16 publications

References 25 publications

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

Sambucus nigra extracts inhibit infectious bronchitis virus at an early point during replication

Sambucus nigra Extracts Inhibit Infectious Bronchitis Virus at an Early Point during Replication

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