Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse

Weyand, Nathan J.; Ma, Mancheong; Phifer‐Rixey, Megan; Taku, Nyiawung A.; Rendón, María A.; Hockenberry, Alyson M.; Kim, Won Jung; Agellon, Al; Biais, Nicolas; Suzuki, Tomohiro; Goodyer-Sait, Lily; Harrison, Odile B.; Bratcher, Holly B.; Nachman, Michael W.; Maiden, Martin C. J.; So, Magdalene

doi:10.1099/ijsem.0.001237

Cited by 33 publications

(46 citation statements)

References 62 publications

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“…The order Oxyurida includes three families namely Oxyuridae (Cobbold, 1864); Pharyngodonidae (Travassos, 1919); and Heteroxynematidae (Skrjabin and Schikhobalova, 1948). Nematodes from the genera Syphacia and Aspiculuris are common parasitic pinworms of rodents all over the world (Adamson, 1994;Robles and Navone, 2007;Millazzo et al, 2010;Sotillo et al, 2012;Verma et al, 2013;Weyand et al, 2016;Zarei et al, 2016;Stewart et al, 2017). Based on morphological characters, the oxyurid species described here showed the characteristic features of the genus Aspiculuris, including the presence of four distinct cephalic papillae lying on the cephalic plate, and three small rudimental lips that carry two sessile poorly developed labial papillae.…”

Section: Discussionmentioning

confidence: 79%

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

Abdel‐Gaber

Abdel-Ghaffar

Quraishy

et al. 2018

Journal of Nematology

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Aspiculuris tetraptera is a heteroxynematid nematoda infecting most of the laboratory animals, occasionally mice which represent the mostly used animal for biological, medical, and pharmacological studies. The present study aimed to investigate the prevalence of nematode parasites infection in the laboratory mice Mus musculus in Egypt. Morphologically, this oxyurid possessed four distinct cephalic papillae on a cephalic plate, with three small rudimental lips carrying two sessile poorly developed labial papillae and one pair of amphidial pores. Esophagus divided into cylindrical corpus and globular bulb. Distinct cervical alae interrupted at the level of esophago-intestinal junction forming an acute angle. At the caudal end, twelve caudal papillae in male worms while an ovijector apparatus opening and a vulva surrounded by protruded lips in females were observed. The general morphological criteria include this nematode with other Aspiculuris species which were compared in the present study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to A. tetraptera recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.

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Section: Discussionmentioning

confidence: 79%

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

Abdel‐Gaber

Abdel-Ghaffar

Quraishy

et al. 2018

Journal of Nematology

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“…The genus Neisseria contains a large number of genetically related species ( 11 ). The vast majority of these are commensals of hosts ranging from rodents, canids, and bovines to nonhuman primates and humans ( 12 – 17 ). Neisseria gonorrhoeae and Neisseria meningitidis are the only two species that cause disease.…”

Section: Introductionmentioning

confidence: 99%

A Natural Mouse Model for Neisseria Colonization

Powell

Weyand

et al. 2018

Infect Immun

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Commensals are important for the proper functioning of multicellular organisms. How a commensal establishes persistent colonization of its host is little understood. Studies of this aspect of microbe-host interactions are impeded by the absence of an animal model. We have developed a natural small animal model for identifying host and commensal determinants of colonization and of the elusive process of persistence. Our system couples a commensal bacterium of wild mice, Neisseria musculi, with the laboratory mouse. The pairing of a mouse commensal with its natural host circumvents issues of host restriction. Studies are performed in the absence of antibiotics, hormones, invasive procedures, or genetic manipulation of the host. A single dose of N. musculi, administered orally, leads to long-term colonization of the oral cavity and gut. All mice are healthy. Susceptibility to colonization is determined by host genetics and innate immunity. For N. musculi, colonization requires the type IV pilus. Reagents and powerful tools are readily available for manipulating the laboratory mouse, allowing easy dissection of host determinants controlling colonization resistance. N. musculi is genetically related to human-dwelling commensal and pathogenic Neisseria and encodes host interaction factors and vaccine antigens of pathogenic Neisseria. Our system provides a natural approach for studying Neisseria-host interactions and is potentially useful for vaccine efficacy studies.

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“…The genes required for Tfp biogenesis and function are present in animal-and human-dwelling Neisseria species (Marri et al, 2010;Weyand et al, 2013Weyand et al, , 2016. Tfp gene expression has been studied mostly in the two pathogenic species.…”

Section: Discussionmentioning

confidence: 99%

RpoN and the Nps and Npa two‐component regulatory system control pilE transcription in commensal Neisseria

Rendón

Lona

et al. 2018

MicrobiologyOpen

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Over 20 genes are involved in the biogenesis and function of the Neisseria Type IV pilus (Tfp). In the pathogenic species, RpoD and the integration host factor (IHF) protein regulate expression of pilE, encoding the Tfp structural subunit. We previously reported that in commensal species, pilE transcription is regulated by RpoN, IHF, and activator Npa. Npa has many hallmarks of response regulators in two-component regulatory systems, leading us to search for its response regulator partner. We report that Npa partners with sensor kinase Nps to control pilE transcription. Among the genes involved in Tfp biogenesis and function, only pilE is controlled by RpoN and Npa/Nps. We summarize our findings in a model, and discuss the implications of the differential regulation of pilE the context of Neisseria Tfp biogenesis.

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Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse

Cited by 33 publications

References 62 publications

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

A Natural Mouse Model for Neisseria Colonization

RpoN and the Nps and Npa two‐component regulatory system control pilE transcription in commensal Neisseria

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