RpoN and the Nps and Npa two‐component regulatory system control <i>pilE</i> transcription in commensal <i>Neisseria</i>

Rendón, María A.; Lona, Beatriz; Ma, Mancheong; So, Magdalene

doi:10.1002/mbo3.713

Cited by 4 publications

(2 citation statements)

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“…In N. musculi, the noncoding DNA segment between the divergently transcribed cps Region A and C contains several potential regulatory sites. The presence of putative Npa and Sigma54 recognition sequences strongly suggests that N. musculi capsule expression is dependent on environmental cues [107,108]. This idea is further supported by the presence of a 17 bp direct repeat upstream of the capsule transport region.…”

Section: Plos Pathogensmentioning

confidence: 80%

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

et al. 2022

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The mechanisms used by human adapted commensal Neisseria to shape and maintain a niche in their host are poorly defined. These organisms are common members of the mucosal microbiota and share many putative host interaction factors with Neisseria meningitidis and Neisseria gonorrhoeae. Evaluating the role of these shared factors during host carriage may provide insight into bacterial mechanisms driving both commensalism and asymptomatic infection across the genus. We identified host interaction factors required for niche development and maintenance through in vivo screening of a transposon mutant library of Neisseria musculi, a commensal of wild-caught mice which persistently and asymptomatically colonizes the oral cavity and gut of CAST/EiJ and A/J mice. Approximately 500 candidate genes involved in long-term host interaction were identified. These included homologs of putative N. meningitidis and N. gonorrhoeae virulence factors which have been shown to modulate host interactions in vitro. Importantly, many candidate genes have no assigned function, illustrating how much remains to be learned about Neisseria persistence. Many genes of unknown function are conserved in human adapted Neisseria species; they are likely to provide a gateway for understanding the mechanisms allowing pathogenic and commensal Neisseria to establish and maintain a niche in their natural hosts. Validation of a subset of candidate genes confirmed a role for a polysaccharide capsule in N. musculi persistence but not colonization. Our findings highlight the potential utility of the Neisseria musculi-mouse model as a tool for studying the pathogenic Neisseria; our work represents a first step towards the identification of novel host interaction factors conserved across the genus.

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Section: Plos Pathogensmentioning

confidence: 80%

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

et al. 2022

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“…The N. meningitidis strains can produce polysaccharide capsules and exopolysaccharides (Table 2) with immunogenic properties and as a tool associated with pathogenicity, virulence, and typing of the bacterial strains, while N. gonorrhoea strains cannot produce immunogenic polysaccharide capsules. However, the capsule production studies suggested that N. gonorrhoea strains may express unusual capsule layer, such as polyphosphate, which may be involved in N. gonorrhoea infection but their prevention has not been demonstrated yet (56).…”

Section: Capsular Polysaccharides and Serotypingmentioning

confidence: 99%

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Tavana

Ataee

Jonaidi

et al. 2020

Jundishapur J Microbiol

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Context: Neisseria meningitidis is the causative agent of a life-threatening infection with high mortality and morbidity worldwide. The most common types of this bacterium are serogroups A, B, C, W135, X, and Y. Although in some countries, such as Iran, the meningococcal meningitis has been well monitored and controlled by the use of divalent and quadrivalent vaccines, other fatal infections caused by these bacteria are still an important threat. For the above reason, this review focused on the differences of Neisseria characteristics, particularly in capsular composition, pathogenic and commensal stages to a better understanding of how to manage Neisseria infections. Evidence Acquisition: In this review, PubMed, EMBASE, ScienceDirect, Scopus, and Google Scholar were searched for English-language publications on pathogenic or commensal strains of Neisseria, meningococcal disease, Neisseria biology, genetic diversity, molecular typing, serogroups, diagnostic, and epidemiology around the world up to July 2019. All articles and academic reports in the defined area of this research were considered too. The data were extracted and descriptively discussed. Results: We included 85 studies in the survey. The data analysis revealed that the distribution of meningococcal serogroups was different regionally. For example, the serogroups C and W-135 accounted for Africa and Latin America regions, serogroup B in the European countries, and rarely in the Western Pacific, and serogroups A and C were dominant in Asian countries. Although data set for laboratory-based diagnosis of N. meningitidis are available for all countries, only 30% of the countries rely on reference laboratories for serogroup determination, and more than half of the countries lack the ability of surveillance system. Nevertheless, molecular detection procedure is also available for all countries. The use of the meningococcal vaccine is a variable country by country, but most countries have applied the meningococcal vaccine, either divalent or quadrivalent, for the protection of high-risk groups. Conclusions: Owing to the geographical distribution of N. meningitidis serogroups in circulating, each country has to monitor for changes in serogroups diversity and its control management. Furthermore, laboratories should scale up the epidemiology and disease burden. It should be mentioned that quadrivalent meningococcal vaccines reduce the meningococcal disease burden sharply.

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Contribution of σ ⁷⁰ and σ ^N Factors to Expression of Class II pilE in Neisseria meningitidis

2019

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Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that undergo antigenic variation or have a class II pilE locus and express invariant pilins. The transcriptional regulation of class I pilE has been studied in both N. meningitidis and Neisseria gonorrhoeae, while the control of expression of class II pilE has been elucidated in the nonpathogenic species Neisseria elongata. However, the factors that govern the regulation of the class II pilE gene in N. meningitidis are not known. In this work, we have bioinformatically and experimentally identified the class II pilE promoter. We confirmed the presence of conserved σ70 and σN-dependent promoters upstream of pilE in a collection of meningococcal genomes and demonstrated that class II pilE expression initiates from the σ70 family-dependent promoter. By deletion or overexpression of sigma factors, we showed that σN, σH, and σE do not affect class II pilin expression. These findings are consistent with a role of the housekeeping σD in expression of this important component of Tfp. Taken together, our data indicate that the σ-dependent network responsible for the expression of class II pilE has been selected to maintain pilE expression, consistent with the essential roles of Tfp in colonization and pathogenesis. IMPORTANCE The type four pilus (Tfp) of Neisseria meningitidis contributes to fundamental processes such as adhesion, transformation, and disease pathology. Meningococci express one of two distinct classes of Tfp (class I or class II), which can be distinguished antigenically or by the major subunit (pilE) locus and its genetic context. The factors that govern transcription of the class II pilE gene are not known, even though it is present in isolates that cause epidemic disease. Here we show that the transcription of class II pilE is maintained throughout growth and under different stress conditions and is driven by a σ70-dependent promoter. This is distinct from Tfp regulation in nonpathogenic Neisseria spp. and may confer an advantage during host-cell interaction and infection.

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RpoN and the Nps and Npa two‐component regulatory system control pilE transcription in commensal Neisseria

Cited by 4 publications

References 63 publications

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Contribution of σ ⁷⁰ and σ ^N Factors to Expression of Class II pilE in Neisseria meningitidis

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RpoN and the Nps and Npa two‐component regulatory system control pilE transcription in commensal Neisseria

Cited by 4 publications

References 63 publications

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Contribution of σ 70 and σ N Factors to Expression of Class II pilE in Neisseria meningitidis

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Contribution of σ ⁷⁰ and σ ^N Factors to Expression of Class II pilE in Neisseria meningitidis