Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow

Martin, Douglas R.; Cox, Nancy R.; Hathcock, Terri; Niemeyer, Glenn P.; Baker, Henry J.

doi:10.1016/s0301-472x(02)00864-0

Cited by 249 publications

(212 citation statements)

References 32 publications

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“…Moreover, the mRNA expression of Sox9, a key transcription factor in chondrocyte differentiation, 44 was markedly upregulated in cell aggregates. These results are consistent with those observed for MSCs isolated from a variety of different species including human, 42 canine, 45 rat, 46 mouse, 47 cat 48 and rabbit. 13 Oligonucleotide-directed gene repair in MSCs is potentially attractive therapeutic approach for selected inherited disorders.…”

Section: Discussionsupporting

confidence: 91%

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

et al. 2008

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Synthetic oligodeoxynucleotides (ODNs) had been employed in gene modification and represent an alternative approach to 'cure' genetic disorders caused by mutations. To test the ability of ODN-mediated gene repair in bone marrow-derived mesenchymal stem cells (MSCs), we established MSCs cell lines with stably integrated mutant neomycin resistance and enhanced green fluorescent protein reporter genes. The established cultures showed morphologically homogenous population with phenotypic and functional features of mesenchymal progenitors. Transfection with gene-specific ODNs successfully repaired targeted cells resulting in the expression of functional proteins at relatively high frequency approaching 0.2%. Direct DNA sequencing confirmed that phenotype change resulted from the designated nucleotide correction at the target site. The position of the mismatchforming nucleotide was shown to be important structural feature for ODN repair activity. The genetically corrected MSCs were healthy and maintained an undifferentiated state. Furthermore, the genetically modified MSCs were able to engraft into many tissues of unconditioned transgenic mice making them an attractive therapeutic tool in a wide range of clinical applications.

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Section: Discussionsupporting

confidence: 91%

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

et al. 2008

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“…The fibroblastoid morphology observed in our in vitro studies showed morphology similar to that reported in cats 36 , as well as to descriptions of rodents and humans 37 . Tropel et al 34 The characterization essays performed for goat CMT showed that these cells also expressed the Nanog gene.…”

Section: Cell Culturesupporting

confidence: 90%

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Filho¹,

Neto²,

Carvalho³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) -Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers -Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 10 6 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.Key words: Cell Culture Techniques. Cell Differentiation. Stem Cells. Flow Cytometry. Goats. Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

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“…MSCs used in these studies have been sourced from various species, including humans and small experimental animals such as rat, mouse, and rabbit, and large animals such as dog, cat, pig, and horse (Kadiyala et al 1997;Martin et al 2002;Ringe et al 2002;Fortier et al 1998;Pittenger et al 1999). However, limited information regarding bovine MSCs is available, despite the wide application of the bovine experimental model in both in vivo and in vitro experiments, particularly in research on chondrocyte metabolism and cartilage resurfacing.…”

Section: Introductionmentioning

confidence: 99%

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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The bone marrow harbors a population of mesenchymal stem cells (MSCs) that possess the potential to differentiate into bone, cartilage, and fat, and along other tissue pathways. To date, MSCs from various species have been studied. Despite the bovine experimental model being widely used in experiments in vivo and in vitro, only a limited amount of information regarding bovine MSCs is available. The aim of this study was to isolate and induce the multilineage mesenchymal differentiation of bovine MSCs, thereby initiating further research on these cells. Bovine MSCs were isolated from eight calves, and osteogenic, chondrogenic, and adipogenic differentiation was induced by using a combination of previously reported protocols for other species. The level of differentiation was evaluated by histological examination and by analyzing the expression of tissue-specific genes by a quantitative "real time" reverse transcription/polymerase chain reaction technique. Following osteoinduction, the isolated fibroblast-like cells transformed into cuboidal cells and formed alkaline-phosphatase-positive colonies; during differentiation, these colonies transformed into mineralized nodules. In addition, osteogenesis was followed by osteocalcin and collagen type I mRNA expression. Chondrogenesis was confirmed by the demonstration of collagen type II, aggrecan, and sox9 mRNA expression in the cells stimulated by transforming growth factor β1 in monolayer culture. After being cultured in an adipogenesis-inducing medium, the MSCs responded by the accumulation of lipid vacuoles and the expression of adipocyte-specific genes. We have therefore demonstrated that cells harvested from bovine bone marrow are capable of in vitro extensive multiplication and multilineage differentiation, making them a relevant and invaluable model in the field of stem cell research.

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Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow

Cited by 249 publications

References 32 publications

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

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