Isolation and characterization of microsatellite loci in Sisyrinchium (Iridaceae) and cross amplification in other genera

Abstract

ABSTRACT. Recent phylogenetic studies on Sisyrinchium strongly suggest that species classified in section Hydastylus and section Viperella belong to a single group of plants in recent adaptive radiation (Clade IV). These species neither present clear morphological differentiation among them nor show clear identification using DNA barcode markers. Thus, the main goal of this study was to develop a set of polymorphic microsatellite markers compatible for representative species of both sections to ensure variability that could be revealed by SSR markers. Therefore, microsatellite primers were isolated and characterized for Sisyrinchium palmifolium and S. marchioides. In addition, transferability of the developed primers was tested in Iridoideae, primarily in closely related species of Sisyrinchium. Sixteen microsatellite loci were developed from enriched genomic libraries, of which ten were polymorphic. G ST values indicated higher differentiation among subpopulations of S. palmifolium than those from S. marchioides. Major transferability was obtained using primers isolated from S. marchioides. All primers exhibited higher rates of cross-amplification for species belonging to Clade IV of Sisyrinchium, as well as to the genera Calydorea and Herbertia. These developed microsatellite markers can be used as an efficient tool for characterization of genetic variability in species belonging to Iridoideae, as well as for studies on population dynamics, genetic structure, and mating system in other Sisyrinchium species.

Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

“…, 2016 ). In the family Iridaceae, for example, a success of 77% was observed in cross-amplification between genera ( Miz et al , 2016 ). In the family Malvaceae cross-genera SSR transferability varied from 71% to 92% ( Satya et al , 2016 ), while in Euphorbiaceae these percentages ranged from 9.5% to 59.1% ( Whankaew et al , 2011 ).…”

Development and characterization of microsatellite markers for endangered species Stipa pennata (Poaceae) and their usefulness in intraspecific delimitation

“…They are the most popular markers used to determine the genetic diversity and differentiation of populations of rare and endangered species [11–15]. Moreover, some studies have postulated their suitability for phylogenetic reconstruction and taxonomic delimitation [16–18]. To the best of our knowledge, microsatellite markers were specifically developed only for two species of Stipa – S. breviflora Griseb.…”