Isolation and characterization of microsatellite loci in smallmouth bass, Micropterus dolomieu (Teleostei: Centrarchidae), and cross‐species amplification in spotted bass, M. punctulatus

Malloy, Terrence P.; Bussche, Ronald A. Van Den; Coughlin, Warren D.; Echelle, Anthony A.

doi:10.1046/j.1365-294x.2000.01096-16.x

Cited by 22 publications

(9 citation statements)

References 4 publications

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“…We genotyped specimens using seven dinucleotide microsatellite DNA markers specifically developed to amplify across black bass species ( Mdo03 : Malloy et al. ; Msaf01 , Msaf05 , Msaf06 , Msaf14 , Msaf17 , and Msaf29 : Seyoum et al. ).…”

Section: Methodsmentioning

confidence: 99%

Hybridization and Genetic Structure of Neosho Smallmouth Bass in the Ozark Highlands

Taylor

Long

Schwemm

et al. 2018

N American J Fish Manag

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The Neosho Smallmouth Bass Micropterus dolomieu velox is endemic to Arkansas River tributaries originating in the Ozark Highlands and Boston Mountains. Although morphologically and genetically distinct from other populations of Smallmouth Bass M. dolomieu, the conservation-genetic status of Neosho Smallmouth Bass is largely unknown. To assist in filling this data gap, we quantified introgressive hybridization, genetic diversity, and population structure of Neosho Smallmouth Bass in two major river basins (Grand River and Illinois River) using seven polymorphic microsatellite markers. Introgressive hybridization with stocked Tennessee lake-strain Smallmouth Bass was most prevalent in the Illinois River, wherein the overall genomic proportion of Neosho Smallmouth Bass alleles was only 0.422. After accounting for hybrid individuals, genetic diversity of Neosho Smallmouth Bass was generally higher in larger rivers and lower in smaller, isolated streams. Three distinct population clusters were identified at the uppermost level of genetic structureone from the Illinois River basin and two from the Grand River basin. These three population boundaries accounted for approximately 7% of the hierarchical genetic variation within our data set, and substructure below the uppermost level accounted for an additional 2% of genetic variation. The population structure we discovered can provide a blueprint for management that conserves diversity within and among populations; for example, population boundaries can be used to determine brood source locations for supplemental stocking efforts to counter nonnative introgression. Introgressive hybridization with nonnative Smallmouth Bass appears to be the most pertinent threat to the Neosho Smallmouth Bass subspecies, which is of conservation value to the overall adaptability of Smallmouth Bass as a species.Smallmouth Bass Micropterus dolomieu is native to a broad region of North America, spanning portions of the upper Mississippi, Tennessee, Ohio, and Saint Lawrence-Great Lakes river basins (Scott and Crossman 1973;Brewer and Orth 2015). Across its range, the Smallmouth Bass inhabits a variety of habitats including streams,

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Section: Methodsmentioning

confidence: 99%

Hybridization and Genetic Structure of Neosho Smallmouth Bass in the Ozark Highlands

Taylor

Long

Schwemm

et al. 2018

N American J Fish Manag

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“…The polymerase chain reaction (PCR) was used to amplify allelic length variants from eight microsatellite loci developed for smallmouth bass and other centrarchid species (e.g. DeWoody & Avise 2000; Malloy et al . 2000; Neff et al .…”

Section: Methodsmentioning

confidence: 99%

Broad‐ to fine‐scale population genetic patterning in the smallmouth bassMicropterus dolomieuacross the Laurentian Great Lakes and beyond: an interplay of behaviour and geography

2007

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Analysis of population genetic relationships reveals the signatures of current processes such as spawning behaviour and migration, as well as those of historical events including vicariance and climate change. This study examines these signatures through testing broad- to fine-scale genetic patterns among smallmouth bass Micropterus dolomieu spawning populations across their native Great Lakes range and outgroup areas, with fine-scale concentration in Lake Erie. Our primary hypotheses include whether genetic patterns result from behavioural and/or geographical isolation, specifically: (i) Are spawning groups in interconnected waterways genetically separable? (ii) What is the degree of isolation across and among lakes, basins, and tributaries? (iii) Do genetic divergences correspond to geographical distances? and (iv) Are historical colonization patterns from glacial refugia retained? Variation at eight nuclear microsatellite DNA loci are analysed for 666 smallmouth bass from 28 locations, including 425 individuals in Lake Erie; as well as Lakes Superior, Huron, and Ontario, and outgroups from the Mississippi, Ohio, St. Lawrence, and Hudson River drainages. Results reveal marked genetic differences among lake and river populations, as well as surprisingly high divergences among closely spaced riverine sites. Results do not fit an isolation-by-geographical-distance prediction for fine-scale genetic patterns, but show weak correspondence across large geographical scales. Genetic relationships thus are consistent with hypotheses regarding divergent origins through vicariance in glacial refugia, followed by colonization pathways establishing modern-day Great Lakes populations, and maintenance through behavioural site fidelity. Conservation management practices thus should preserve genetic identity and unique characters among smallmouth bass populations.

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“…Microsatellite optimization and genotyping.-Polymerase chain reaction (PCR) optimization and amplification was attempted for 45 microsatellite loci originally isolated in other centrarchids (Colbourne et al 1996;Neff et al 1999;Malloy et al 2000). Reactions were 10 lL, consisting of 13 buffer containing 0.75-1.75 mM MgCl 2 , 0.04 lM of an M13 (À29) 5'-adapted forward primer, 0.20 lM of a reverse primer, 0.20 lM of 25% labeled M13 (À29) infrared dye 700/800 sequence (LI-COR, Inc., Lincoln, Nebraska), 0.20 mM of each deoxynucleotide triphosphate, 10-20 ng of genomic DNA, and 0.25 U Taq DNA polymerase (Promega, Madison, Wisconsin).…”

Section: Methodsmentioning

confidence: 99%

Admixture Analysis of Florida Largemouth Bass and Northern Largemouth Bass using Microsatellite Loci

et al. 2006

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Microsatellite DNA variation was examined at 11 loci in five populations of Florida largemouth bass Micropterus salmoides floridanus (N ¼ 175) and eight populations of northern largemouth bass M. s. salmoides (N ¼ 249). Distinct allele frequencies with 33 private alleles between subspecies (threshold frequency, !0.05) and 19 private alleles among three geographic regions distinguished between Florida largemouth bass and northern largemouth bass as well as between northern largemouth bass from northern and southern latitudes in North America. Variation at microsatellite loci also provided sufficient resolution to discriminate among some populations within regions. Allele frequencies indicated that of 37 trophy bass (defined here as fish weighing 5.90 kg or more) donated to the Texas Parks and Wildlife Department (TPWD) between 2004 and 2005, all had more than 50% Florida largemouth bass influence with ancestry genetically similar to that of populations sampled in western Florida. Some fish (N ¼ 24) were direct descendents of Florida largemouth bass (either remnants or direct descendants from introductions), whereas others (N ¼ 13) were admixed with northern largemouth bass. Of the 13 admixed fish, 11 had ancestry in lineages of southernlatitude northern largemouth bass. Genetic variation within northern largemouth bass populations was depressed at northern latitudes (mean heterozygosity, 0.37; SD, 0.26; mean number of alleles per locus, 2.91; SD, 1.51) relative to southern latitudes (mean heterozygosity, 0.52; SD, 0.25; mean number of alleles per locus, 4.57; SD, 2.88); Florida largemouth bass exhibited intermediate heterozygosity (mean, 0.41; SD, 0.32) and an allelic richness (mean, 4.51; SD, 4.58) similar to that of southern-latitude northern largemouth bass. Overall, the variation observed at these loci is greater than that at other codominant markers explored in this species, providing additional power to detect admixture in populations and individuals.

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Isolation and characterization of microsatellite loci in smallmouth bass, Micropterus dolomieu (Teleostei: Centrarchidae), and cross‐species amplification in spotted bass, M. punctulatus

Cited by 22 publications

References 4 publications

Hybridization and Genetic Structure of Neosho Smallmouth Bass in the Ozark Highlands

Hybridization and Genetic Structure of Neosho Smallmouth Bass in the Ozark Highlands

Broad‐ to fine‐scale population genetic patterning in the smallmouth bassMicropterus dolomieuacross the Laurentian Great Lakes and beyond: an interplay of behaviour and geography

Admixture Analysis of Florida Largemouth Bass and Northern Largemouth Bass using Microsatellite Loci

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