Admixture Analysis of Florida Largemouth Bass and Northern Largemouth Bass using Microsatellite Loci

Abstract

Microsatellite DNA variation was examined at 11 loci in five populations of Florida largemouth bass Micropterus salmoides floridanus (N ¼ 175) and eight populations of northern largemouth bass M. s. salmoides (N ¼ 249). Distinct allele frequencies with 33 private alleles between subspecies (threshold frequency, !0.05) and 19 private alleles among three geographic regions distinguished between Florida largemouth bass and northern largemouth bass as well as between northern largemouth bass from northern and southern latitudes in North America. Variation at microsatellite loci also provided sufficient resolution to discriminate among some populations within regions. Allele frequencies indicated that of 37 trophy bass (defined here as fish weighing 5.90 kg or more) donated to the Texas Parks and Wildlife Department (TPWD) between 2004 and 2005, all had more than 50% Florida largemouth bass influence with ancestry genetically similar to that of populations sampled in western Florida. Some fish (N ¼ 24) were direct descendents of Florida largemouth bass (either remnants or direct descendants from introductions), whereas others (N ¼ 13) were admixed with northern largemouth bass. Of the 13 admixed fish, 11 had ancestry in lineages of southernlatitude northern largemouth bass. Genetic variation within northern largemouth bass populations was depressed at northern latitudes (mean heterozygosity, 0.37; SD, 0.26; mean number of alleles per locus, 2.91; SD, 1.51) relative to southern latitudes (mean heterozygosity, 0.52; SD, 0.25; mean number of alleles per locus, 4.57; SD, 2.88); Florida largemouth bass exhibited intermediate heterozygosity (mean, 0.41; SD, 0.32) and an allelic richness (mean, 4.51; SD, 4.58) similar to that of southern-latitude northern largemouth bass. Overall, the variation observed at these loci is greater than that at other codominant markers explored in this species, providing additional power to detect admixture in populations and individuals.

Discovery and validation of gene‐linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)

“…Genotyping was conducted based on a subset of microsatellite markers from Lutz‐Carrillo et al . () and Seyoum et al . ().…”

“…Subsets of each population were previously genotyped using diagnostic microsatellite markers (Lutz‐Carrillo et al . ; Seyoum et al . ).…”

“…; Johnson & Fulton ; Lutz‐Carrillo et al . , ; Barthel et al . ; Dumont & Lutz‐Carrillo ; Austin et al .…”

Conservation of Black Bass Diversity: An Emerging Management Paradigm

“…The native Largemouth Bass had previously been identified as a unique lineage that was genetically divergent from other Largemouth Bass populations and exhibited glossohyal teeth, a morphological trait rarely observed in Largemouth Bass (Lutz‐Carrillo et al. ). Therefore, the diversity lost to introgression could not be recovered by reintroducing fish from any other drainage.…”

Population genetic structure of North American burbot (Lota lota maculosa) across the Nearctic and at its contact zone with Eurasian burbot (Lota lota lota)

“…Note that the microsatellite admixture is calculated at the population level, while mtDNA admixture refers to the frequency of Lota l. lota (EB lineage) or L. l. maculosa (NA lineage) mtDNA in individuals in the population. Rhymer et al 2001;Fedorov et al 2003), though subspecies of closely related temperate fish are often found to interbreed freely at contact zones (reviewed in Utter 2001;Lutz-Carrillo et al 2006;Taylor et al 2007). In fact, interbreeding when no geographic barrier prevails is fundamental to the definition of fish subspecies (Nelson 1999;Haig et al 2006).…”