Isolation and characterization of microsatellite markers for the ornamental discus fish <i>Symphysodon discus</i> and cross‐species amplification in other Heroini cichlid species

Amado, Manuella Villar; Hrbek, Tomas; Gravena, Waleska; Fantin, Cleiton; Assunção, Enedina Nogueira de; Astolfi-Filho, Spártaco; Farias, Izeni Pires

doi:10.1111/j.1755-0998.2008.02200.x

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…Eight microsatellites ( Amado et al 2008 ; Passos et al 2010 ) were mapped using (FISH) fluorescence in situ hybridization during the mitotic metaphase of Cichla monoculus , Pterophyllum scalare , and Symphysodon discus (Table 1 ). The repetitive sequence probes were labeled with digoxigenin-11-dUTP or biotin-16-dUTP (Dig-Nick Translation Mix and BioNick Translation Mix; Roche) using nick translation reactions following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)

Schneider¹,

Gross²,

Terencio³

et al. 2015

CCG

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Fish of the family Cichlidae are recognized as an excellent model for evolutionary studies because of their morphological and behavioral adaptations to a wide diversity of explored ecological niches. In addition, the family has a dynamic genome with variable structure, composition and karyotype organization. Microsatellites represent the most dynamic genomic component and a better understanding of their organization may help clarify the role of repetitive DNA elements in the mechanisms of chromosomal evolution. Thus, in this study, microsatellite sequences were mapped in the chromosomes of Cichla monoculus Agassiz, 1831, Pterophyllum scalare Schultze, 1823, and Symphysodon discus Heckel, 1840. Four microsatellites demonstrated positive results in the genome of Cichla monoculus and Symphysodon discus, and five demonstrated positive results in the genome of Pterophyllum scalare. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the centromeric or telomeric regions, which suggests that sequences contribute to chromosome structure and may have played a role in the evolution of this fish family. The comparative genome mapping data presented here provide novel information on the structure and organization of the repetitive DNA region of the cichlid genome and contribute to a better understanding of this fish family’s genome.

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Section: Methodsmentioning

confidence: 99%

Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)

Schneider¹,

Gross²,

Terencio³

et al. 2015

CCG

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“…To characterize Symphysodon individuals, we used 13 microsatellite pairs developed by Amado et al [46]. Genotyping was done according to the economical method of Schuelke [47] that uses a 5′ tailed amplification primer, and then a third fluorescently labeled primer in the genotyping reaction.…”

Section: Methodsmentioning

confidence: 99%

A Molecular Perspective on Systematics, Taxonomy and Classification Amazonian Discus Fishes of the GenusSymphysodon

Amado

Farias

Hrbek

2011

International Journal of Evolutionary Biology

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With the goal of contributing to the taxonomy and systematics of the Neotropical cichlid fishes of the genus Symphysodon, we analyzed 336 individuals from 24 localities throughout the entire distributional range of the genus. We analyzed variation at 13 nuclear microsatellite markers, and subjected the data to Bayesian analysis of genetic structure. The results indicate that Symphysodon is composed of four genetic groups: group PURPLE—phenotype Heckel and abacaxi; group GREEN—phenotype green; group RED—phenotype blue and brown; and group PINK—populations of Xingú and Cametá. Although the phenotypes blue and brown are predominantly biological group RED, they also have substantial contributions from other biological groups, and the patterns of admixture of the two phenotypes are different. The two phenotypes are further characterized by distinct and divergent mtDNA haplotype groups, and show differences in mean habitat use measured as pH and conductivity. Differences in mean habitat use is also observed between most other biological groups. We therefore conclude that Symphysodon comprises five evolutionary significant units: Symphysodon discus (Heckel and abacaxi phenotypes), S. aequifasciatus (brown phenotype), S. tarzoo (green phenotype), Symphysodon sp. 1 (blue phenotype) and Symphysodon sp. 2 (Xingú group).

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“…Additionally, this study provides a group of 16 C. kraussii species-specific microsatellite loci with long repetition motifs (4-mer, 5-mer) and are highly polymorphic since its PIC values > 0.5 were above the range proposed by Botstein and company [115]. Both the diversity levels and the PIC values of the loci here developed were found within the range of the loci designed for other neotropical cichlids which repetition motifs are mostly short (2-mer) and compound [33,[56][57][58][59][60][61][62][63][64]. Additionally, loci here developed exhibited amplification consistency and high definition of the electropherograms that ease the allele assignation and, thus, their genotypification, for which they are considered informative and appropriate for the C. kraussii population genetics study.…”

Section: Discussionmentioning

confidence: 56%

“…To contrast these hypotheses, microsatellite loci were developed as a molecular tool to assess this species genetic diversity and structure. This approach was used for avoiding heterologous loci-related genotyping errors [52][53][54][55], considering that to date microsatellite loci have been developed for phylogenetically distant neotropical cichlids like Amphilophus cichlasoma [56], Symphysodon discus [57], Astronotus crassipinis [58], Cichla piquiti [59], Cichla monoculus [60], Cichla temensis and Cichla orinocensis [61], Geophagus brasiliensis [62] Apistogramma agassizii [63], Apistogramma gephyra [64] and Pterophyllum scalarae [33].…”

Section: Introductionmentioning

confidence: 99%

Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

Cataño Tenorio,

Joya,

Márquez

2024

PLoS ONE

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The adaptative responses and divergent evolution shown in the environments habited by the Cichlidae family allow to understand different biological properties, including fish genetic diversity and structure studies. In a zone that has been historically submitted to different anthropogenic pressures, this study assessed the genetic diversity and population structure of cichlid Caquetaia kraussii, a sedentary species with parental care that has a significant ecological role for its contribution to redistribution and maintenance of sedimentologic processes in its distribution area. This study developed de novo 16 highly polymorphic species-specific microsatellite loci that allowed the estimation of the genetic diversity and differentiation in 319 individuals from natural populations in the area influenced by the Ituango hydroelectric project in the Colombian Cauca River. Caquetaia kraussii exhibits high genetic diversity levels (Ho: 0.562–0.885; He: 0.583–0.884) in relation to the average neotropical cichlids and a three group-spatial structure: two natural groups upstream and downstream the Nechí River mouth, and one group of individuals with high relatedness degree, possibly independently formed by founder effect in the dam zone. The three genetic groups show recent bottlenecks, but only the two natural groups have effective population size that suggest their long-term permanence. The information generated is relevant not only for management programs and species conservation purposes, but also for broadening the available knowledge on the factors influencing neotropical cichlids population genetics.

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Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross‐species amplification in other Heroini cichlid species

Cited by 6 publications

References 8 publications

Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)

Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)

A Molecular Perspective on Systematics, Taxonomy and Classification Amazonian Discus Fishes of the GenusSymphysodon

Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

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