Isolation and characterization of microsatellite markers in the newly discovered invasive fruit fly pest in Africa, Bactrocera invadens (Diptera: Tephritidae)

Abstract

We describe the isolation and characterization of 11 polymorphic microsatellite loci from the recently discovered fruit fly pest, Bactrocera invadens. The polymorphism of these loci was tested in individual flies from two natural populations (Sri Lanka and Democratic Republic of Congo). Allele number per locus ranged from three to 15 and eight loci displayed a polymorphic information content greater than 0.5. These microsatellite loci provide useful markers for studies of population dynamics and invasion history of this pest species.

Population structure of a global agricultural invasive pest, Bactrocera dorsalis (Diptera: Tephritidae)

“…() and Khamis et al. (). Fluorescently labeled fragments were visualized on ABI PRISM 377 Genetic Analyzer with ROX‐500 size standard.…”

“…Eleven microsatellite loci were used in this study: MS3, MS3B, MS4, MS5, MS6, Bd9, Bd19, Bd42, Bp198, Bp200, Bi5; technical details are given in Dai, Lin, and Chang (2004), Aketarawong, Bonizzoni, Malacrida, Gasperi, and Thanaphum (2006), Shearman et al (2006) and Khamis et al (2008). Fluorescently labeled fragments were visualized on ABI PRISM 377 Genetic Analyzer with ROX-500 size standard.…”

Towards area‐wide control of Bactrocera invadens: prospects of the sterile insect technique and molecular entomology

“…Microsatellite markers have been isolated, and assessment of the population structure of B. invadens has shown that different subpopulations of this pest exist in different sub‐Saharan Africa regions with levels of genetic diversities . This finding is of importance to area‐wide control strategies such as SIT targeted against this pest, as it could influence the outcome of such a program.…”

Trapping and the Detection, Control, and Regulation of Tephritid Fruit Flies

“…), B. dorsalis(Dai et al 2004;Aketarawong et al 2006;Shearman et al 2006;Li et al 2007), Bactrocera invadens Drew, Tsuruta & White(Khamis et al 2008), B. cucurbitaeWu et al 2011;Jacquard et al 2013), and Bactrocera cacuminata (Hering)(Song et al 2006); Ceratitis: C. capitataCasey and Burnell 2001;Meixner et al 2002;Stratikopoulos et al 2009), Ceratitis anonae Graham, Ceratitis rosa Karsch, and Ceratitis fasciventris (Bezzi); and Rhagoletis: Rhagoletis pomonella (Walsh)(Velez et al 2006), Rhagoletis indifferens Curran(Maxwell et al 2009), and Rhagoletis completa Cresson(Chen et al 2006). Although primers were developed to amplify a specific locus in the target species, several studies have demonstrated that microsatellite primers can amplify the microsatellite from multiple species (e.g.,Baliraine et al 2003;Velez et al 2006;Stratikopoulos et al 2009;Islam et al 2011;Drosopoulou et al 2011).Published protocols for microsatellite loci have been used to study a wide range of biological questions.…”