Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus

Pereira, Mayra Ramos de Jesus; Pinhatti, Valéria Rodrigues; Silveira, Maiele Dornelles; Matzenbacher, Cristina Araujo; Freitas, Thales Renato Ochotorena de; Silva, Juliana da; Camassola, Melissa; Nardi, Nance Beyer

doi:10.1590/1678-4685-gmb-2018-0012

Cited by 4 publications

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“…Values are mean ± standard deviation of three independent experiments Fig. 4 Clonogenic ability of MSC cultures (cc1 to cc5), expressed as mean percentage of plated cells able to originate colonies ± standard deviation et al 2017, respectively), and adipose tissue from humans (Markarian et al 2014), and from a wild rodent species, Ctenomys minutus (Pereira et al 2018). The reasons for restricted differentiation potential of ccMSCs observed in the present study are not clear, and further studies would be necessary to identify a biological reason for that, or the existence of subpopulations with different adipogenic potential.…”

Section: Discussionmentioning

confidence: 99%

Coronary corium, a new source of equine mesenchymal stromal cells

et al. 2020

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Mesenchymal stromal cells (MSCs) have attracted great attention for therapeutic applications. Since cells derived from different tissues have different properties, using the right tissue source may impact their efficiency in regenerative medicine. This study describes for the first time the isolation and characterization of MSCs derived from the equine coronary corium, which may be useful for treating diseases such as laminitis. Seven coronary corium samples were used for isolation of cells (ccMSCs). Adherent cells were characterized for morphology, immunophenotype, proliferation and differentiation potential, in vitro migration and colony-forming capacity. The cells displayed the characteristic fibroblastoid morphology, with population doubling time increasing until passage 7 and reaching a plateau in passage 10. Cells were negative for CD14 and CD45, and positive for CD73 and CD90. ccMSCs showed chondrogenic and osteogenic, but not adipogenic differentiation, and migrated with nearly total closing of the empty area in 48 h, in the scratch assay. The clonogenic potential was in average 18% to 23%. This study describes for the first time the establishment of mesenchymal stromal cell cultures from the equine coronary corium. The results are similar to MSCs isolated from many other equine tissues, except for restricted differentiation potential. As coronary corium stem cell regulation may contribute to the pathogenesis of equine chronic laminitis, the use of ccMSCs in cell therapy for this significantly debilitating disease should be further investigated.

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Section: Discussionmentioning

confidence: 99%

Coronary corium, a new source of equine mesenchymal stromal cells

et al. 2020

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“…Mesenchymal stem cells (MSCs) are types of multipotential adult stem cells that are characterized by high proliferation capacity, self-renewal, low immunogenicity, and multilineage differentiation. MSCs can be isolated from bone marrow (BMSCs), umbilical cord (UC-MSCs), peripheral blood (PB-MSCs), placenta, teeth (dental pulp stem cells, DPSCs), and adipose tissue (AD-MSCs) [ 1 ]. They can be induced in vitro to differentiate into neurocytes, chondrocytes, osteoblasts, adipocytes, and many other cell types [ 2 ].…”

Section: Introductionmentioning

confidence: 99%