MSC based gene delivery methods and strategies improve the therapeutic efficacy of neurological diseases

Zhou, Heng; He, Yan; Xiong, Wei; Jing, Shuili; Duan, Xingxiang; Huang, Zhiyun; Nahal, Gurek S.; Peng, Yulin; Li, Mingchang; Zhu, Yingbo; Ye, Qingsong

doi:10.1016/j.bioactmat.2022.11.007

Cited by 25 publications

(27 citation statements)

References 322 publications

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“…Despite their high therapeutic potential, the clinical application of siRNAs is greatly limited due to the lack of an effective delivery system. 38,39 Therefore, finding suitable delivery systems is crucial to enhance the effectiveness of siRNA. Alginate is an anionic polymer derived from brown algae or bacteria, consisting of repeating units of linearly linked β- D -mannuronic acid (M) and α- l -guluronic acid (G) via 1,4-glycosidic bonds.…”

Section: Discussionmentioning

confidence: 99%

Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance

Lu,

Zhong,

Gao

et al. 2024

J Biomater Appl

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The long non-coding RNA HOTAIR and the Hedgehog-Gli1 signaling pathway are closely associated with tumor occurrence and drug resistance in various cancers. However, their specific roles in the development of EGFR-TKIs resistance in non-small cell carcinoma remain unclear. To address the issue of EGFR-TKIs resistance, this study utilized the electrospray method to prepare sodium alginate microspheres encapsulating HOTAIR siRNA (SA/HOTAIR siRNA) and investigated its effects on RNA interference (RNAi) in the gefitinib-resistant cell line PC9/GR. Furthermore, the study explored whether HOTAIR could modulate EGFR-TKIs resistance through the Hedgehog-GLi1 signaling pathway. The experimental results showed that sodium alginate (SA) microspheres demonstrated excellent biocompatibility with high encapsulation efficiency and drug-loading capacity, effectively enhancing the silencing efficiency of siRNA. HOTAIR siRNA significantly inhibited the proliferation, migration, and invasion abilities of PC9/GR cells while promoting apoptosis. Additionally, HOTAIR siRNA effectively suppressed tumor growth and downregulated the Hedgehog-GLi1 pathway and anti-apoptotic proteins, which were confirmed in animal experiments. Moreover, SA/HOTAIR siRNA exhibited superior inhibition of cellular and tumor functions compared to using HOTAIR siRNA alone. Clinical research findings indicated that monitoring the expression level of HOTAIR in the serum and urine samples of NSCLC patients before and after receiving EGFR-TKIs treatment can predict the efficacy of EGFR-TKIs to a certain extent. This study provided evidence that HOTAIR siRNA effectively mitigated the development of acquired resistance to EGFR-TKIs by inhibiting the Hedgehog-GLi1 pathway. Furthermore, it introduced a reliable and long-lasting drug delivery system for combating acquired resistance to EGFR-TKIs.

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Section: Discussionmentioning

confidence: 99%

Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance

Lu,

Zhong,

Gao

et al. 2024

J Biomater Appl

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“…68 Zhou et al used ZIF-8 to support dental pulp stem cells and then loaded them into gelatin methylacrylyl hydrogels and injected them into the injured spinal cord of rats to achieve effective nerve repair. 69…”

Section: Mofs Materials In Neural Tissue Engineeringmentioning

confidence: 99%

Metal–organic framework-based platforms for implantation applications: recent advances and challenges

Liu,

Wang,

Quan

et al. 2024

J. Mater. Chem. B

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“…The methodology exhibited a high level of biocompatibility with the neural stem cell lineage and demonstrated ease in its binding to fibroblast growth factor, a critical element for the sustenance of neural stem cells. 52 Wu et al 53 used a combination of the chemical ferulic acid (FA) and glycolic CS (a CS precursor that acts as a water-soluble) to create neuroprotective nanoparticles (Nps). The combination of glycol CS and FA has the potential to generate hydrophobically self-assembled Nps.…”

Section: Chitosan-based Polysaccharide Drug Delivery Systems For Repa...mentioning

confidence: 99%

“…Heparin, a well‐known anionic glycosaminoglycan that stops blood from clotting reduces inflammation, and has a high binding affinity for growth factor receptors, was used to bind CS ionically. The methodology exhibited a high level of biocompatibility with the neural stem cell lineage and demonstrated ease in its binding to fibroblast growth factor, a critical element for the sustenance of neural stem cells 52 . Wu et al 53 used a combination of the chemical ferulic acid (FA) and glycolic CS (a CS precursor that acts as a water‐soluble) to create neuroprotective nanoparticles (Nps).…”

Section: The Current State Of Polysaccharide‐based Drug Delivery Syst...mentioning

confidence: 99%

Management of peripheral nerve injuries using natural based biomaterials and their derivatives: Advances and prospective

Kumar,

Malviya,

Sundram

2024

MedComm – Biomaterials and Applications

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The management of peripheral nerve injuries is an important concern due to the their incidence of nerve lesions and inappropriate regeneration that follows severe injuries, which ultimately reduces the lives of patients with this condition. Different strategies have been investigated to repair severe nerve injuries with the improvement of motor and sensory regeneration. Although autograft remains the gold standard technique, an emerging number of research articles concerning nerve conduit use have been reported in the last few years. Nerve conduits aim to overcome autograft disadvantages, but they satisfy some requirements to be suitable for nerve repair. A universal ideal conduit does not exist since conduit properties have to be evaluated case by case; nevertheless, because of their high biocompatibility and biodegradability, natural‐based biomaterials have great potential to be used to produce nerve guides. Although they have many characteristics with synthetic biomaterials, natural‐based biomaterials are preferable because of their extraction sources; indeed, these biomaterials are obtained from different renewable sources or food waste, thus reducing environmental impact and enhancing sustainability in comparison to synthetic ones. This review highlights the recent progress in the development of natural‐based biomaterials and their derivatives for the management of peripheral nerve injuries.

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MSC based gene delivery methods and strategies improve the therapeutic efficacy of neurological diseases

Cited by 25 publications

References 322 publications

Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance

Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance

Metal–organic framework-based platforms for implantation applications: recent advances and challenges

Management of peripheral nerve injuries using natural based biomaterials and their derivatives: Advances and prospective

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