Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast

Abdelfattah, Mohamed S.; Elmallah, Mohammed I. Y.; Hawas, Usama W.; El-Kassem, Lamia T. Abou; Eid, Mennat Allah G.

doi:10.1016/j.apjtb.2016.06.004

Cited by 49 publications

(26 citation statements)

References 28 publications

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“…Messaoudi et al (2015) mentioned that microbes from soils are the most important natural sources exhibiting strong biological activity against a wide range of pathogens; similarly, current investigation involved isolation from soil sediment. Abdelfattah et al (2016) considered that seas and oceans are a valuable source for microorganisms and bioactive secondary metabolites; in accordance, study isolation habitat was a sea. Orlova , et al (2015) examined marine actinomycetes as producers of secondary bioactive metabolites; as well, marine actinomycetes were the under test study target.…”

Section: Discussionmentioning

confidence: 92%

“…Moreover, present findings of isolate tolerance to temperature as thermotolerant, pH, NaCl as halotolerant, complex compounds degradation ability and biochemical characters, as well confirmed the taxonomy of Saccharomonospora based on Bergey's Manual (Whitman et al, 2012) . Actinomycetes were identified to genus and species levels in almost resent studies depending on molecular examinations (Sengupta et al, 2015;Abdelfattah et al, 2016 andGanesan et al, 2017). Current molecular revolution seems to give us a more objective tool in taxonomy, first of all based on the characterization of the rRNA genes.…”

Section: Discussionmentioning

confidence: 99%

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Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

Ibrahim

2017

Egypt. J. Microbiol.

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T HIS WORK is aimed to assess the antibiosis efficacy of marine thermophilic actinomycetes isolated from raw marine habitat and identify the most antibiosis effective isolate. Variable thermophilic actinomycete strains were isolated from 14 marine sediment samples at Burj Al Arab, El-Agamy salinases and Cairo-Alex route Alexandria, Egypt. The isolated strains were purified and screened for their antibiosis activities against various targets of human pathogenic bacteria and fungi. Actinomycete isolate, characterized by highest broad-spectrum antagonism, was selected and identified based on morphological, cultural, physiological and biochemical characteristics. Total cell hydrolysate analysis, Genomic DNA extraction and 16S rRNA sequencing were also characterized. Among the purified 135 marine thermophilic actinomycete isolates, twenty strains showed detectable antagonistic activities against test pathogens. Based on identification collected data, it was suggested that the most potent undertreat isolate is belonging to actinomycete genus Saccharomonospora. Molecular studies revealed that it was highly related to Saccharomonospora viridis (100%). As a result, it was designated as Saccharomonospora viridis AHK190.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

Ibrahim

2017

Egypt. J. Microbiol.

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“…respectively while, in our study isolate (49) which was molecularly identified as a strain of Sulfitobacter sp. and isolates (74) and (130) which were identified as strains of Nocadiopsis sp., found to possess three different types of biosynthetic genes (PKS-1, II and NRPS) [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates

Samak

Solyman

Hanora

2018

Biotechnology Reports

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“…Forty-seven actinomycete strains were picked up, subsequently spread on waksman agar plates and labeled with the code numbers EGY1 to EGY47. Characterizations of the isolated strains were carried by morphological methods (33) and on the basis of 16S rRNA partial gene sequences (34).…”

Section: Methodsmentioning

confidence: 99%

Marine actinomycete crude extracts with potent TRAIL-resistance overcoming activity against breast cancer cells

et al. 2017

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Abstract. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent, as it can kill tumor cells selectively. In our search of bioactive natural products to overcome TRAIL-resistance, we isolated 47 actinomycete strains from different sediments and seawater samples collected from the Red Sea coast in Egypt and found four crude extracts (EGY1, EGY3, EGY24 and EGY34) displaying TRAIL sensitizing activity in the resistant breast cancer cell line MDA-MB-231. None of these crude extracts exhibited cytotoxic effect on normal mouse embryonic fibroblasts (MEF), with the exception of EGY34. Analysis of the signaling pathways underlying the sensitization of MDA-MB-231 cells to TRAIL-induced apoptosis, by western blotting, revealed that all crude extracts facilitated initiator caspase-8/-10 activation upon TRAIL stimulation, but that in addition, EGY3 and EGY34, alone, induced strong ER-stress activation, with the appearance of BiP in the cytosolic extracts. Our results pave the way to the discovery and the development of marine-derived drugs for cancer therapy.

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Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast

Cited by 49 publications

References 28 publications

Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates

Marine actinomycete crude extracts with potent TRAIL-resistance overcoming activity against breast cancer cells

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