Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates

Abstract

HighlightsRevealing the biodiversity of Red Sea marine invertebrates associated microorganisms by 16S rRNA sequence analysis.Isolation of bacteria and actinomycetes with antimicrobial activity from different Red Sea invertebrates.Detection of biosynthetic gene clusters (PKS and NRPS) in microorganisms associated with the Red Sea marine invertebrates.

Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling

“…The presence of biosynthetic gene clusters (BGC) was screened to understand the ability of the prospective fungi to produce bioactive polyketides and non-ribosomal peptides. Some studies demonstrated that the presence of biosynthetic gene cluster (BGC) such as polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) genes in microorganisms is correlated to their capability to inhibit the growth of other microorganisms (Passari et al 2016;Böhringer et al 2017;Samak et al 2018). We detected the existence of PKS-I, PKS-II and NRPS genes in Eutypella sp.…”

Presence of Biosynthetic Gene Clusters (NRPS/PKS) in Actinomycetes of Mangrove Sediment in Semarang and Karimunjawa, Indonesia

“…Type I PKS gene cluster detection was used Thermo Scientific 2X Phire Plant Direct PCR Master Mix with MDPQQR f (5'-RTRGAYCCNCAGCAIC G-3') and HGTGT r (5'-VGTNCCNGTGCCRTG-3') primer pair (El Samak et al, 2018). The PCR condition was 98°C for 5 min for initial denaturation; then 10 cycles consisting of a denaturation stage at 98°C for 5 sec, annealing stage at 60°C (temperature reduced 2°C per cycle) for 5 sec, extension stage at 72°C for 1 min, followed with 30 cycles with a denaturation stage at 98°C for 1 min, annealing stage at 40°C for 5 sec, extension stages at 72°C for 1 min, and a final extension stage at 72°C for 1 min and a cooling stage at 4°C.…”

“…The PCR condition was 98°C for 5 min for initial denaturation; then 10 cycles consisting of a denaturation stage at 98°C for 5 sec, annealing stage at 60°C (temperature reduced 2°C per cycle) for 5 sec, extension stage at 72°C for 1 min, followed with 30 cycles with a denaturation stage at 98°C for 1 min, annealing stage at 40°C for 5 sec, extension stages at 72°C for 1 min, and a final extension stage at 72°C for 1 min and a cooling stage at 4°C. PKS-II gene amplification was carried out by mixing the primary pair of PF6 (5'-TSGCSTGCTTGG AYGCSATC-3 ') and PR6 (5'TGGAANCCGCCGAA BCCGCT-3') (El Samak et al, 2018), 1 µL each at a concentration of 10 mM, with 1 µL of extracted template DNA, 10 µL of Thermo Scientific2X Phire Plant Direct PCR Master Mix, and 8 µL of ddH2O. PCR amplification process was carried out in 40 cycles with the following stages: initial denaturation stage (98°C, 5 min), followed by denaturation (98°C, 5 sec), annealing (70°C, 5 sec), extension stage (72°C, 1 min), and the final extension (72°C, 1 min).…”

The ocean genome and future prospects for conservation and equity

“…Perhaps the hallmark of human benefit from a marine gene is the discovery of green fluorescent protein, which produces bioluminescence in the jellyfish Aequorea victoria and has been used across a range of applications from protein tagging to identifying levels of environmental toxicity, contributions ultimately recognized with the 2008 Nobel Prize in Chemistry 27 . Further examples include bioprospecting for novel antifoulants and adhesives, and the search for novel antibiotics, which has increasingly focused on the bioactive compounds produced by marine invertebrates and microorganisms associated with sea sponges [28][29][30] . Other marine microorganisms produce a type of naturally occurring polymer (extracellular polymeric substance) that is of interest in bioremediation efforts due to its capacity to detoxify pollutants such as heavy metals 31 , while Pseudomonas spp.…”