Isolation and characterization of mammalian orthoreovirus type 3 from a fecal sample from a wild boar in Japan

Zhang, Wenjing; Kataoka, Michiyo; Doan, Yen Hai; Oi, Toru; Furuya, Tetsuya; Oba, Mami; Mizutani, Tetsuya; Oka, Takahiro; Li, Tiancheng; Nagai, Makoto

doi:10.1007/s00705-021-05053-7

Cited by 6 publications

(7 citation statements)

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“…Recombination events in viruses promote adaptation to a novel host species range and increase their pathogenicity [47]. Our previous study revealed a possible intrasegment recombination event in the M2 gene of the Japanese wild boar MRV strain Wild boar/To14/JPN/2018 with a lion strain from the Japanese zoo and bat strains [20]. In the present study, RDP detected crossover points in the M1 gene of Toyama14 with Totto-MoI-6 and Ishi-Ueno-10 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, the S1 gene of Kana-Uchi-15 diverged from that of other strains (≥89.1% and ≥88.3% nucleotide and amino acid sequence identities, respectively; Table 1). Notably, the S1 genes of Kana-Ebina-9 and Kana-Ebina-11 had high sequence identities (98.4% and 98.7% nucleotide and amino acid sequence identities, respectively) with MRV-3 WB/To14 strain isolated from the Japanese wild boar in Toyama prefecture, the central region of the main island, in 2018 [20]. Phylogenetic analysis of the L1 gene showed that Totto-MoI6, Kana-Uchi-15, and Ishi-Ueno-10 branched with porcine MRVs from the USA, Taiwan, and Italy (Fig.…”

Section: Phylogenetic Analysis and Pairwise Nucleotide Comparisonmentioning

confidence: 99%

“…However, the reports do not contain any genetic information on the MRVs, and sequence data for porcine MRV in Japan are not available. Recently, we isolated MRV type 3 (MRV-3) from a wild boar in Japan and performed genetic analyses [20]. Furthermore, while investigating swine enteric viruses, we isolated five MRVs from pig fecal samples using cell culture.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples

et al. 2022

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Mammalian orthoreovirus (MRV) is a non-enveloped double-stranded RNA virus with a broad host range. MRVs are prevalent worldwide and have been isolated from various hosts, including humans, dogs, cats, wild boars, sewage, and pigs, in Japan. However, Japanese porcine MRV has not been genetically characterized yet. While investigating porcine enteric viruses including MRV, five MRVs were isolated from the feces of Japanese pigs using the MA104 cell culture. Genetic analysis of the S1 gene revealed that the Japanese porcine MRV strain isolates could be classified as MRV-2 and MRV-3.Whole genome analysis showed that Japanese porcine MRVs exhibited genetic diversity, although they shared homology with porcine MRV sequences deposited in the DDBJ/EMBL/GenBank database. Several potential intra-genetic reassortment events among MRV strains from pigs, sewage, and humans have been detected in Japan, suggesting zoonotic transmission. Furthermore, homologous recombination events were identified in the M1 and S1 genes of Japanese porcine MRV. These findings imply that different strains of Japanese porcine MRVs share a porcine MRV genomic backbone and have evolved through intra-genetic reassortment and homologous recombination events. Fig. 1C. Fukase et al. LC613213.1 Mammalian orthoreovirus 2 THK0325 MN639758.1 Mammalian orthoreovirus strain 19/242 MN788298.1 Mammalian orthoreovirus strain HLJYC2017 JX486061.1 Mammalian orthoreovirus 3 isolate GD-1 KY419124.1 Mammalian orthoreovirus 3 isolate ZJ2013 KT224508.1 Mammalian orthoreovirus 3 strain SD-14 MK092968.1 Mammalian orthoreovirus 2 isolate OV204 Orthoreovirus Ebina9-2021 M2MARC145 Orthoreovirus Ebina11-2021 M2MARC145 KT444556.1 Mammalian orthoreovirus isolate WIV7 mu-1 gene complete cds KT444536.1 Mammalian orthoreovirus isolate WIV4 mu-1 gene complete cds KC462153.1 Mammalian orthoreovirus isolate HB-A LC121914 Panthera leo KX263311.1 Mammalian orthoreovirus 1 isolate B/03 MG457102.1 Mammalian orthoreovirus isolate SI-MRV04 KU194670.1 Mammalian orthoreovirus 2 isolate T3/Pipistrellus kuhlii/Italy/5515-3/2012 GQ468269.1 Mammalian orthoreovirus 3 isolate MPC/04 LC476919.1 Mammalian orthoreovirus 2 Osaka2014 GU196310.1 Mammalian orthoreovirus 2 MRV2Tou05 LC476899.1 Mammalian orthoreovirus 2 Osaka1994 DQ482462.1 Mammalian orthoreovirus isolate SC-A TY14-M2 LC476909.1 Mammalian orthoreovirus 2 Osaka2005 gene for mu-1 protein complete cds MK408599.1 Mammalian orthoreovirus isolate MRV-3 chamois 84407 Italy 2009 KT444546.1 Mammalian orthoreovirus isolate WIV5 KU194662.1 Mammalian orthoreovirus 3 T3/Pipistrellus kuhlii/Italy/5515-2/2012 JQ412759.1 Mammalian Orthoreovirus strain T3/Bat/Germany/342/08 KX932033.1 Mammalian orthoreovirus isolate mew716 MRV-3 MG457082.1 Mammalian orthoreovirus isolate SI-MRV02 KF154728.1 Mammalian orthoreovirus isolate SI-MRV01 JX415471.1 Porcine reovirus SHR-A M20161.1 Reovirus type 3 Dearing U24260.1 Reovirus type 3 Abmey MN022935.1 Mammalian orthoreovirus strain AP-151/BR KX384850.1 Mammalian orthoreovirus strain MORV/47Ma/06 MG457112.1 Mammalian o...

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Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysis and Pairwise Nucleotide Comparisonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples

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“…The viral RNA genome was extracted from the supernatants with the use of a QlAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany), in accordance with the manufacturer's instructions. The RNA was amplified by using reverse transcription polymerase chain reaction (RT-PCR) for the detection of L1 gene of MRVs with virus-specific primers as shown in Table 1 [40]. Just before RT-PCR assay, 2 μL of extracted RNA was mixed with 1 μL of dimethyl sulfoxide (DMSO) (Wako Pure Chemical Industries, Co., Ltd., Osaka, Japan) and 4.5 μL of redistilled water (dW2), then heated at 98℃ for 5 min.…”

Section: Rt-pcr Testingmentioning

confidence: 99%

Isolation, molecular characterization, and disinfectants susceptibility of swine-carried mammalian orthoreoviruses in Japan in 2020–2022

Miyaoka

Kadota

Kabir

et al. 2023

The Journal of Veterinary Medical Science

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Biosecurity enhancement contributes to the reduction of various microbial pathogens. Mammalian orthoreoviruses (MRVs) which are increasingly recognized as potentially serious problems on swine industry were used as indicators of biosecurity enhancement on two pig farms. Twelve MRVs were detected and isolated from fecal specimens of healthy pigs collected from one of two farms in Japan.By sequencing based on the partial S1 gene, MRV isolates were classified as MRV1 and MRV2.Additionally, the virucidal activities of disinfectants toward the isolated MRV1 were evaluated using quaternary ammonium compound (QAC) diluted 500 times with water (QAC-500), 0.17% food additive glade calcium hydroxide (FdCa(OH)2) solution, QAC diluted with 0.17% FdCa(OH)2 solution (Mix-500), sodium hypochlorite at 100 or 1,000 parts per million (ppm) of total chlorine (NaClO-100 or NaClO-1000, respectively). To efficiently inactivate MRV1 (≥ 3 log10 reductions), 0.17% FdCa(OH)2, Mix-500 and NaClO-1000 required 5 min, whereas it took 30 min for QAC-500. The number of MRV detections has decreased over time, after using Mix-500 for disinfection on the positive farm. These results suggest that different serotypes of MRVs are circulating among pigs, and that the occurrence of MRVs in the farms decreased consequent to more effective disinfection.

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“…According to cell‐attachment capability, type‐specific antisera and hemagglutinin activities of σ1 protein encoded by S1 segment, MRVs are assigned to four serotypes: type 1 Lang (T1L), type 2 Jones (T2J), type 3 Dearing (T3D), and putative 4 Ndelle 3 . MRVs have a wide geographic distribution and can infect numerous mammals including humans, bats, and domestic animals such as swine and bovine as well as wild mammals 4–12 . MRV human infections normally cause respiratory 2,13 and enteric diseases 14–16 and neurological signs in some cases 17–19 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of mammalian orthoreovirus from bats in the United States

Wang

Zheng

Shen

et al. 2023

Journal of Medical Virology

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Mammalian orthoreovirus (MRV) infects many mammalian species including humans, bats, and domestic animals. To determine the prevalence of MRV in bats in the United States, we screened more than 900 bats of different species collected during 2015–2019 by a real‐time reverse‐transcription polymerase chain reaction assay; 4.4% bats tested MRV‐positive and 13 MRVs were isolated. Sequence and phylogenetic analysis revealed that these isolates belonged to four different strains/genotypes of viruses in Serotypes 1 or 2, which contain genes similar to those of MRVs detected in humans, bats, bovine, and deer. Further characterization showed that these four MRV strains replicated efficiently on human, canine, monkey, ferret, and swine cell lines. The 40/Bat/USA/2018 strain belonging to the Serotype 1 demonstrated the ability to infect and transmit in pigs without prior adaptation. Taken together, this is evidence for different genotypes and serotypes of MRVs circulating in US bats, which can be a mixing vessel of MRVs that may spread to other species, including humans, resulting in cross‐species infections.

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Isolation and characterization of mammalian orthoreovirus type 3 from a fecal sample from a wild boar in Japan

Cited by 6 publications

References 63 publications

Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples

Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples

Isolation, molecular characterization, and disinfectants susceptibility of swine-carried mammalian orthoreoviruses in Japan in 2020–2022

Isolation and characterization of mammalian orthoreovirus from bats in the United States

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