Isolation and Characterization of <i>Escherichia coli</i> from Animals, Humans, and Environment

Abstract

Working on a diverse species of bacteria that have hundreds of pathotypes representing hundreds of strains and many closely related family members is a challenge. Appropriate research design is required not only to achieve valid desired outcome but also to minimize the use of resources, including time to outcome and intervention. This chapter outlines basics of Escherichia coli isolation and characterization strategies that can assist in research designing that matches the set objectives. Types of samples to be collected, collection and storage strategies, and processing of samples are described. Different approaches to isolation, confirmation and concentration of various E. coli strains are summarized in this chapter. Characterization and typing of E. coli isolates by biochemical, serological, and molecular methods have been explained so that an appropriate choice is made to suite a specific E. coli strain/pathotype. Some clues on sample and isolate preservation for future use are outlined, and general precautions regarding E. coli handling are also presented to the researcher to avoid improper planning and execution of E. coli-related research. Given different options, the best E. coli research design, however, should try as much as possible to shorten the length of time to outcomes.

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

“…But this finding is lower than some reports from previous works (13). The prevalence of E. coli from cloacae in this study is in line with the privies studies [14,15]. In contrast the finding of this prevalence in the present study is higher than the study of [16,22,23,24 ] in which E. coli was isolated from cloacae samples taken from poultry farms.…”

Study on Bacteria Isolates and Antimicrobial Resistance in Wildlife in Sicily, Southern Italy

“…Swabs and feces from live animals and tissue samples collected from carcasses were subjected to bacteriological procedures to isolate bacterial species. Bacteriological cultures were performed using selective and differential medium (blood agar, MacConkey agar, and mannitol salt agar, Thermo Fisher Scientific, Waltham, MA USA) to promote the growth of the main commensal and pathogenic species from the collected samples [ 19 , 20 ]. For the isolation of Salmonella spp.…”

Effects of Pasteurization and High-Pressure Processing of Camel and Bovine Cheese Quality, and Proteolysis Contribution to Camel Cheese Softness

“…Staphylococcus aureus was enumerated on Baird Parker agar supplemented with egg yolk according to ( 20 ). Listeria monocytogenes were detected according to ( 21 ) while the Escherichia coli was examined with MacConkey agar followed by 24 h incubation at 37°C according to ( 22 ).…”

“…Listeria monocytogenes were detected according to (21) while the Escherichia coli was examined with MacConkey agar followed by 24 h incubation at 37 • C according to (22). Lactose, protein, fats, and total solids contents (%) were evaluated using Near Infra-Red Multipurpose Analyzer (MPA), Bruker Optik Gmbh (Ettlingen, Germany) (23).…”