Isolation and Characterization of Large Marine Bacteriophage (Myoviridae), VhKM4 Infecting Vibrio harveyi

Abstract

The causative agent responsible for vibriosis in tropical fish aquaculture, Vibrio harveyi, has become a major bacterial pathogen. Studies suggest that this bacterium has developed resistance to antibiotics commonly used in aquaculture. In view of this situation and the requirement for the proposed postantibiotic era, bacteriophage therapy seems to be a promising control strategy for fish vibriosis. In this study, a lytic Vibrio phage VhKM4 belonging to a member of large, marine Myoviridae was successfully isolated. It exhibited bacteriolysis to both V. harveyi VHJR7 and V. parahaemolyticus ATCC 17802. The latent period of the VhKM4 phage was recorded at 60 min. It also recorded average burst size of approximately 52 plaque-forming units per infected cell. A strong bacteriolytic activity at low multiplicity of infection of 0.01 indicates the effectiveness of this large marine myovirid against fish pathogenic strain of V. harveyi VHJR7. Received June 16, 2016; accepted October 7, 2016.

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

“…The eclipse time, latent period, and the burst size were about 10 min, approximately 50 min and 133 ± 23 PFU/cell, respectively ( Figure 2D). Compared to literature values, the latent period for LPSEYT is shorter than other myoviruses, such as Salmonella phage vB_SnwM_CGG4-2 (about 58 min), Vibrio harveyi phage VhKM4 (about 60 min), and Stenotrophomonas maltophilia phage φSMA5 (about 80 min) [39][40][41]. At the same time, its burst size was greater than VhKM4 (about 52 PFU/cell), phSE-1 (about 28 PFU/mL), and phSE-2 (about 53 PFU/mL) [28,40], which is likely to be a reflection of high lytic activity.…”

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“…To seek a phage specific to a given bacteria, most authors recommend to search in the bacteria's ecosystem. Aquaculture farm water and sediments, diseased animals or heavily contaminated ecosystems like sewage water are often used (Kim et al, 2012;Kim et al, 2010;Letchumanan et al, 2016;Kokkari et al, 2018;Surekhamol et al, 2014;Lal et al, 2017;Phumkhachorn et al, 2010). The rationale behind these strategies is that a phage is more likely to be present in its host ecosystem than anywhere else because of the specificity of phage-host interactions.…”

Application of a Novel Lytic Podoviridae Phage Pu20 for Biological Control of Drug-Resistant Salmonella in Liquid Eggs

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