Isolation and characterization of inulin with a high degree of polymerization from roots of Stevia rebaudiana (Bert.) Bertoni

Lopes, Sheila Mara Sanches; Krausová, Gabriela; Rada, V.; Gonçalves, José Eduardo; Gonçalves, Regina Aparecida Correia; Oliveira, Arildo José Braz de

doi:10.1016/j.carres.2015.03.018

Cited by 59 publications

(19 citation statements)

References 27 publications

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“…The chain lengths of these fructans range from 2 to 60 units, with an average DP of 12. DP influences important properties, such as solubility, thermal stability, sweetness power, and prebiotic activity [83,84]. Standard chicory inulin has an average DP of about 10 to 12.…”

Section: Wall Materialsmentioning

confidence: 99%

“…The main applications of inulin are related to its technological properties, such as a substitute for sugars and fats in low-calorie foods, as a thickener, emulsifier, and gelling agent, and it may constitute a potential auxiliary agent for drying processes [80,84]. In the field of bioactives encapsulation, the use of inulin as wall material enables its health benefits to be harnessed, and at the same time it allows the release of bioactive compounds in the colon due to its resistance to pH variations in the gastrointestinal tract [23,90].…”

Section: Wall Materialsmentioning

confidence: 99%

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Advances in the Application of Microcapsules as Carriers of Functional Compounds for Food Products

2019

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Natural bioactive compounds and living cells have been reported as promising products with beneficial properties to human health. The constant challenge regarding the use of these components is their easy degradation during processing and storage. However, their stability can be improved with the microencapsulation process, in which a compound sensitive to adverse environmental conditions is retained within a protective polymeric material. Microencapsulation is a widely used methodology for the preservation and stabilization of functional compounds for food, pharmaceutical, and cosmetic applications. The present review discusses advances in the production and application of microcapsules loaded with functional compounds in food products. The main methods for producing microcapsules, as well as the classes of functional compounds and wall materials used, are presented. Additionally, the release of compounds from loaded microcapsules in food matrices and in simulated gastrointestinal conditions is also assessed.

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Section: Wall Materialsmentioning

confidence: 99%

Section: Wall Materialsmentioning

confidence: 99%

Advances in the Application of Microcapsules as Carriers of Functional Compounds for Food Products

2019

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“…The medicinal importance of S. rebaudiana is ascribed to the presence of diterpene glycosides including stevioside (3-10%), rebaudioside A (13%) and rebaudioside B, C and D in its leaves (Chaturvedula, Prakash, 2011). These glycosides are 100-300 times sweeter than those of sucrose (Lopes et al, 2015). Diterpene glycosides produced by S. rebaudiana are an approved dietary supplement by the U.S. Food & Drug Administration (2018) and comparatively low-calorie sweeteners due to which they are highly recommended for diabetic and obesity patients (Chan et al, 2000;Lee et al, 2001;Luwańska et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Factors affecting organogenesis of Stevia rebaudiana and in vitro accumulation of steviol glycosides

Blinstrubienė

Burbulis

Juškevičiūtė

et al. 2020

Zemdirbyste-Agriculture

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The aim of this research was to investigate the effect of explant type and cytokinin type and concentration on stevia direct organogenesis in vitro, and to determine the effect of cytokinin type on stevioside and rebaudioside A content in regenerated shoots. Stem and leaf segments of stevia (Stevia rebaudiana Bertoni), taken from 6-week-old in vitro grown plants, were inoculated on Murashige and Skoog (MS) medium supplemented with different (1.0-6.0 µM) concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (KIN) for shoot regeneration. The content of steviol glycosides (stevioside and rebaudioside A) in S. rebaudiana donor plants and regenerated shoots was evaluated using high-performance liquid chromatography (HPLC). Results revealed a significant effect (p < 0.01) of explant type, cytokinin type and cytokinin concentration on shoot regeneration frequency. The mean value of regeneration frequency from stem segments was significantly higher in comparison with leaf segments. Thidiazuron significantly promoted the mean regeneration frequency in comparison to 6-benzylaminopurine and kinetin. The highest mean shoot formation frequency was obtained on the medium supplemented with 3.0 µM of TZD. Incorporation of 4.0 µM BAP and 4.0 µM TZD into culture media resulted in significant increase in rebaudioside A and stevioside ratio, while a decrease in rebaudioside A and stevioside ratio was recorded when the medium was supplemented with 4.0 µM KIN. Rebaudioside A and stevioside ratio in the medium supplemented with 4.0 µM TZD was significantly higher in comparison with 4.0 µM BAP and 4.0 µM KIN.

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“…However, the use of phenol and sulfuric acid is potentially dangerous for operators. Another possible alternative is to use trifluoroacetic acid (TFA), which presents lower acidity compared to sulfuric acid, and is also able to hydrolyze inulin and fructan molecules [21].…”

Section: Introductionmentioning

confidence: 99%

“…After hydrolysis of all fructans to fructose and glucose, inulin quantification can be completed by measuring these sugars by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and high-performance liquid chromatography with refractometer detection (HPLC-RI) [21]. High performance liquid chromatography (HPLC) equipped with evaporative light scattering detector (ELSD) is widely used for quantification of sugar in samples [22,23].…”

Section: Introductionmentioning

confidence: 99%

Development of a Combined Trifluoroacetic Acid Hydrolysis and HPLC-ELSD Method to Identify and Quantify Inulin Recovered from Jerusalem artichoke Assisted by Ultrasound Extraction

Yin

et al. 2018

Applied Sciences

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Featured Application: Due to the growing importance of inulin in food industry, it is of paramount importance to have appropriate techniques to extract this compound from plant food materials, wastes and by-products. Moreover, using simple, reliable and fast identification and quantification methods in order to verify manufacturer's information and for quality control purposes, is also of great interest. Therefore, in the present work, a combined trifluoroacetic acid assisted hydrolysis and high performance liquid chromatography equipped with evaporative light scattering detector technique was developed and validated in order to identify and quantify the amount of inulin extracted assisted by ultrasound processing from Jerusalem artichoke root slices as a model plant food matrix.Abstract: Over the last years, inulin, a fructan mixture consisting of oligosaccharides and polysaccharides, has attracted more and more attention from both food industry and researchers, due to its unique functional properties as a natural resource. Therefore, there is an increased interest in the extraction and quantification of inulin for its valorization from inulin rich plants, wastes and by-products. In this work, ultrasonic treatment was applied for inulin extraction, observing a great impact of extraction temperature and ultrasonic power on the inulin content in the obtained extracts. A combined process including trifluoroacetic acid (TFA)-assisted hydrolysis and analysis with high performance liquid chromatography equipped with evaporative light scattering detector (HPLC-ELSD) was developed to quantify inulin content. The effect of hydrolysis parameters was investigated, obtaining the optimal conditions after using TFA at a concentration of 1 mg/mL, hydrolysis temperature of 90 • C, and hydrolysis duration of 60 min. The good linearity (>0.995), precision, recovery (100.27%), and stability obtained during the validation process showed that this developed method allows the quantification of total inulin content in the samples analyzed. This combined method may also contribute to the investigation of the functional properties of inulin (e.g., as prebiotic).

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Isolation and characterization of inulin with a high degree of polymerization from roots of Stevia rebaudiana (Bert.) Bertoni

Cited by 59 publications

References 27 publications

Advances in the Application of Microcapsules as Carriers of Functional Compounds for Food Products

Advances in the Application of Microcapsules as Carriers of Functional Compounds for Food Products

Factors affecting organogenesis of Stevia rebaudiana and in vitro accumulation of steviol glycosides

Development of a Combined Trifluoroacetic Acid Hydrolysis and HPLC-ELSD Method to Identify and Quantify Inulin Recovered from Jerusalem artichoke Assisted by Ultrasound Extraction

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