Isolation and characterization of <i>MbWRKY2</i> gene involved in enhanced drought tolerance in transgenic tobacco

Han, Deguo; Zhang, Zhaoyuan; Ding, Haibin; Chai, Lijing; Liu, Wei; Gao, Yang

doi:10.1080/17429145.2018.1447698

Cited by 32 publications

(19 citation statements)

References 31 publications

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“…The seedlings cultured under normal Hoagland nutrient solution were as control. After 0, 1, 3, 6, 12, 24 and 48 h for stress treatment, the plant materials were obtained and frozen with liquid nitrogen immediately, then stored in −80 • C for RNA extraction [35].…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Han

Zhou

et al. 2020

IJMS

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NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 • C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS). The proteins encoded by NAC family genes form homodimers or heterodimers at the N-terminal, consist of about 150 amino acids. The N-terminal regions of NAC proteins share a conserved DNA binding region, which is divided into five subdomains (A-E) [8], whereas the C-terminal region that contains a transcriptional regulatory domain is highly diversified.The regulatory mechanism of NAC TFs consists of two types. The first one is the regulation of transcriptional levels including phosphorylation and ubiquitination miRNAs, which can regulate the expression of TFs at the protein level. The other is that post-transcriptional regulation can be carried out by binding to the target mRNA. NAC transcription factors can regulate target genes by binding to NACRS (NAC recognition sequence) or NACBS (NAC binding sequence), thereby affecting fruit appearance, ripeness, aging, flavor and nutritional quality [9,10]. In addition, NAC transcription factors also bind DNA or other protein kinases to regulate growth and development in plants and various physiological processes, including top meristem [11], secondary wall formation [12], flower organ development [13], bud differentiation [14], embryo development [15], lateral root formation [16,17], plant organ aging and fruit maturity [18].Abiotic stress factors such as drought, high salt, cold and low temperature seriously affect the growth of plants. Under these stresses, a large number of reactive oxygen species will be gener...

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Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Han

Zhou

et al. 2020

IJMS

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“…These results showed that the WRKY TFs family was highly conserved during evolution (Rinerson et al 2015). Previous reports have indicated that WRKY TFs genes are widely distributed in Arabidopsis, rice, soybean, maize, cotton, strawberry, orange, grape, and peach, which are known to be involved in abiotic (Ramamoorthy et al 2008;Han et al 2018) and biotic stress responses (Pandey & Somssich 2009), seed development Figure 6. Expression of MbWRKY3 in transgenic tobacco and over-expression of MbWRKY3 in tobacco improved drought stress tolerance.…”

Section: Discussionmentioning

confidence: 69%

“…In vitro grown seedlings of M. baccata were propagated and rooted on MS medium, and transferred to Hoagland's solution for acclimation and growing for 50 d (Han et al 2018). When the seedlings had 8-9 mature leaves (fully expanded), they were exposed to air on filter paper with 20% relative humidity for dehydration and drought treatment.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Studies on WRKY genes predominantly point to an involvement in salicylic acid (SA) signaling and disease responses (Eulgem et al 2000;Asai et al 2002). In addition, WRKY genes are involved in plant responses to drought, heat, cold, salinity (Zhou et al 2008;Liu et al 2014;Sáenz-Mata et al 2014;Cheng et al 2017;Ye et al 2017;Han et al 2018), freezing (Huang & Duman 2002), wounding (Hara et al, 2000), and oxidative stress (Rizhsky et al 2004;Cui et al 2018). But these studies mostly focused on model plants or crops, and the roles of the WRKY genes in stress responses of Malus plant were less well known.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and functional analysis of MbWRKY3 involved in improved drought tolerance in transformed tobacco

Han

Zhang

Ding

et al. 2018

Journal of Plant Interactions

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Plant-specific WRKY transcription factors were involved in stress responses and ABA signaling. In the present study, a WRKY gene is isolated from Malus baccata (L.) Borkh and designated as MbWRKY3. Subcellular localization revealed that MbWRKY3 was localized onto nucleus. The MbWRKY3 expression levels were up-regulated by salinity, drought, and ABA treatments in M. baccata. When MbWRKY3 was introduced into tobaccos, it improved drought stress tolerance in transgenic plants. Compared to WT, the transgenic tobaccos had the higher levels of relative water content, and proline and chlorophyll contents, decreased levels of electrolyte leakage, MDA, and H 2 O 2 , increased activities of the reactive oxygen species-related enzymes (SOD, CAT, and POD), and greater upregulations of the corresponding genes (NtSOD, NtCAT, and NtPOD), especially when dealt with drought stress. These results suggest that MbWRKY3 gene plays a positive regulatory role in drought stress response.

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“…Limited water supply enhances the production of reactive oxygen species (ROS) [68,69], and plants are protected by glutathione S-transferase (GST) and other antioxidant enzymes scavenging excessive ROS from the damage caused by ROS [70]. This is because GST comprises a large superfamily of multifunctional protein and participates in ascorbic acid (AsA)/glutathione (GSH) cycling pathways [68]. Here, probable glutathione S-transferase GST12 was significantly down-regulated under the MD treatment compared with the CK treatment.…”

Section: Reactive Oxygen Scavenging System and Ion Channel In Responsmentioning

confidence: 99%

Transcriptomic Analysis of Female Panicles Reveals Gene Expression Responses to Drought Stress in Maize (Zea mays L.)

Jia

Jiang

et al. 2020

Agronomy

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Female panicles (FPs) play an important role in the formation of yields in maize. From 40 days after sowing to the tasseling stage for summer maize, FPs are developing and sensitive to drought. However, it remains unclear how FPs respond to drought stress during FP development. In this study, FP differentiation was observed at 20 and 30 days after drought (DAD) and agronomic trait changes of maize ears were determined across three treatments, including well-watered (CK), light drought (LD), and moderate drought (MD) treatments at 20, 25, and 30 DAD. RNA-sequencing was then used to identify differentially expressed genes (DEGs) in FPs at 30 DAD. Spikelets and florets were suppressed in LD and MD treatments, suggesting that drought slows FP development and thus decreases yields. Transcriptome analysis indicated that 40, 876, and 887 DEGs were detected in LD/CK, MD/CK, and MD/LD comparisons. KEGG pathway analysis showed that ‘biosynthesis of other secondary metabolites’ and ‘carbohydrate metabolism’ were involved in the LD response, whereas ‘starch and sucrose metabolism’ and ‘plant hormone signal transduction’ played important roles in the MD response. In addition, a series of molecular cues related to development and growth were screened for their drought stress responses.

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Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Cited by 32 publications

References 31 publications

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Molecular cloning and functional analysis of MbWRKY3 involved in improved drought tolerance in transformed tobacco

Transcriptomic Analysis of Female Panicles Reveals Gene Expression Responses to Drought Stress in Maize (Zea mays L.)

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