Transcriptomic Analysis of Female Panicles Reveals Gene Expression Responses to Drought Stress in Maize (Zea mays L.)

Jia, Shuangjie; Li, Hongwei; Jiang, Yanping; Tang, Yulou; Zhao, Guizhe; Zhang, Yinglei; Yang, Siqi; Qiu, Husen; Wang, Yongchao; Guo, Jia; Yang, Qinghua; Shao, Ruixin

doi:10.3390/agronomy10020313

Cited by 10 publications

(8 citation statements)

References 68 publications

(67 reference statements)

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“…KEGG pathway analysis showed that “biosynthesis of other secondary metabolites” and “carbohydrate metabolism” were involved in response to low drought stress, whereas “starch and sucrose metabolism” and “plant hormone signal transduction” played important roles in medium drought response. Notably, three genes, cuc2 (LOC103629107), TE1 (LOC541683), and DLF1 (LOC100037791), emerged as significant players in silk differentiation under drought stress conditions …”

Section: Uncovering Role Of Transcriptomics For Drought and Heat Tole...mentioning

confidence: 99%

“…Notably, three genes, cuc2 (LOC103629107), TE1 (LOC541683), and DLF1 (LOC100037791), emerged as significant players in silk differentiation under drought stress conditions. 124 Similarly, transcriptome interplay during kernel formation from flag leaf and developing kernels observed downregulation of genes corresponding to cell division, formation of anatomical boundaries, and nucleosome assembly alongside genes regulating histone proteins. Cell division was significantly reduced due to downregulation of its corresponding genes, i.e., CYC1;2, CYC2;3, CYC3;1, and CYC3;4 and auxins were most affected plant hormone as indicated by differential expression of auxin responsive genes, i.e., IAA3, IAA7, IAA8, IAA13, IAA26, ARF1, ARF3, ARF6, ARF11, and IBR1.…”

Section: Transcriptomic Insights Into Drought Tolerance Mechanismsmentioning

confidence: 99%

“…The KEGG pathway analysis highlighted that light drought induced biosynthesis of secondary metabolites and carbohydrate metabolism, whereas medium drought induced plant hormone signal transduction and starch and sucrose metabolisms. 124 Likewise, exploring tissue specific expressions is also important during drought stress because different tissues respond differently. Leaves are considered ideal for transcriptomics studies 122,126−128 followed by roots, 115,129 male and female flowers, 130,131 ears, 124 kernels, 125 sperm.…”

Section: Transcriptomic Insights Into Drought Tolerance Mechanismsmentioning

confidence: 99%

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Leveraging Multiomics Insights and Exploiting Wild Relatives’ Potential for Drought and Heat Tolerance in Maize

Jamil,

Ahmad,

Shahzad

et al. 2024

J. Agric. Food Chem.

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Climate change, particularly drought and heat stress, may slash agricultural productivity by 25.7% by 2080, with maize being the hardest hit. Therefore, unraveling the molecular nature of plant responses to these stressors is vital for the development of climate-smart maize. This manuscript's primary objective was to examine how maize plants respond to these stresses, both individually and in combination. Additionally, the paper delved into harnessing the potential of maize wild relatives as a valuable genetic resource and leveraging AI-based technologies to boost maize resilience. The role of multiomics approaches particularly genomics and transcriptomics in dissecting the genetic basis of stress tolerance was also highlighted. The way forward was proposed to utilize a bunch of information obtained through omics technologies by an interdisciplinary state-of-the-art forward-looking bigdata, cyberagriculture system, and AI-based approach to orchestrate the development of climate resilient maize genotypes.

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Section: Uncovering Role Of Transcriptomics For Drought and Heat Tole...mentioning

confidence: 99%

Section: Transcriptomic Insights Into Drought Tolerance Mechanismsmentioning

confidence: 99%

Section: Transcriptomic Insights Into Drought Tolerance Mechanismsmentioning

confidence: 99%

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Leveraging Multiomics Insights and Exploiting Wild Relatives’ Potential for Drought and Heat Tolerance in Maize

Jamil,

Ahmad,

Shahzad

et al. 2024

J. Agric. Food Chem.

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“…The irruption of transcriptomics, metabolomics, high-throughput DNA sequencing and high-density microarrays in the analysis of plants' responses to stress have brought new insights and allowed a better understanding on plants reactions to stressful conditions [21]. The stress-responding genes and their regulation pattern under drought were analyzed in common buckwheat cotyledons and roots [22] and female panicles in maize [23], and under salinity in roots and leaves of pomegranate [24].…”

Section: Drought and Salinitymentioning

confidence: 99%

Physiological and Molecular Characterization of Crop Resistance to Abiotic Stresses

Boscaiu

Fita

2020

Agronomy

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Abiotic stress represents a main constraint for agriculture, affecting plant growth and productivity. Drought and soil salinity, especially, are major causes of reduction of crop yields and food production worldwide. It is not unexpected, therefore, that the study of plant responses to abiotic stress and stress tolerance mechanisms is one of the most active research fields in plant biology. This Special Issue compiles 22 research papers and 4 reviews covering different aspects of these responses and mechanisms, addressing environmental stress factors such as drought, salinity, flooding, heat and cold stress, deficiency or toxicity of compounds in the soil (e.g., macro and micronutrients), and combination of different stresses. The approaches used are also diverse, including, among others, the analysis of agronomic traits based on morphological characteristics, physiological and biochemical studies, and transcriptomics or transgenics. Despite its complexity, we believe that this Special Issue provides a useful overview of the topic, including basic information on the mechanisms of abiotic stress tolerance as well as practical aspects such as the alleviation of the deleterious effects of stress by different means, or the use of local landraces as a source of genetic material adapted to combined stresses. This knowledge should help to develop the agriculture of the (near) future, sustainable and better adapted to the conditions ahead, in a scenario of global warming and environmental pollution.

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“…The complex mechanism of drought tolerance in plants is regulated simultaneously by multiple genes [ 4 ]. RNA-seq offers an effective means to investigate plant resistance and has evolved into a potent tool for elucidating drought stress and predicting gene function [ 5 ]. Furthermore, transcriptome analysis can illuminate the molecular mechanisms involved in specific biological processes [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals regulatory mechanisms of different drought-tolerant Gleditsia sinensis seedlings under drought stress

Liu,

Zhao,

Wang

et al. 2024

BMC Genom Data

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Background Gleditsia sinensis is a significant tree species from both ecological and economic perspectives. However, its growth is hampered by temporary droughts during the seedling stage, thereby impeding the development of the G. sinensis industry. Drought stress and rehydration of semi-annual potted seedlings using an artificial simulated water control method. RNA sequencing (RNA-seq) analyses were conducted on leaves collected from highly resistant (HR) and highly susceptible (HS) seedling families at five different stages during the process of drought stress and rehydration to investigate their gene expression patterns. Results The differentially expressed genes (DEGs) were predominantly enriched in pathways related to “chloroplast” (GO:0009507), “photosynthesis” (GO:0015979), “plant hormone signal transduction” (map04075), “flavonoid biosynthesis” (map00941), “stress response”, “response to reactive oxygen species (ROS)” (GO:0000302), “signal transduction” (GO:0007165) in G. sinensis HR and HS families exposed to mild and severe drought stress. Additionally, the pathways related to “plant hormone signal transduction” (map04075), and osmoregulation were also enriched. The difference in drought tolerance between the two families of G. sinensis may be associated with “transmembrane transporter activity” (GO:0022857), “stress response”, “hormones and signal transduction” (GO:0007165), “cutin, suberine and wax biosynthesis” (map00073), “ribosome” (map03010), “photosynthesis” (map00195), “sugar metabolism”, and others. An enrichment analysis of DEGs under severe drought stress suggests that the drought tolerance of both families may be related to “water-soluble vitamin metabolic process” (GO:0006767), “photosynthesis” (map00195), “plant hormone signal transduction” (map04075), “starch and sucrose metabolism” (map00500), and “galactose metabolism” (map00052). Osmoregulation-related genes such as delta-1-pyrroline-5-carboxylate synthase (P5CS), Amino acid permease (AAP), Amino acid permease 2 (AAP2) and Trehalose-phosphate synthase (TPS), as well as the antioxidant enzyme L-ascorbate peroxidase 6 (APX6), may be significant genes involved in drought tolerance in G. sinensis. Five genes were selected randomly to validate the RNA-seq results using quantitative real-time PCR (RT-qPCR) and they indicated that the transcriptome data were reliable. Conclusions The study presents information on the molecular regulation of the drought tolerance mechanism in G. sinensis and provides a reference for further research on the molecular mechanisms involved in drought tolerance breeding of G. sinensis.

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Transcriptomic Analysis of Female Panicles Reveals Gene Expression Responses to Drought Stress in Maize (Zea mays L.)

Cited by 10 publications

References 68 publications

Leveraging Multiomics Insights and Exploiting Wild Relatives’ Potential for Drought and Heat Tolerance in Maize

Leveraging Multiomics Insights and Exploiting Wild Relatives’ Potential for Drought and Heat Tolerance in Maize

Physiological and Molecular Characterization of Crop Resistance to Abiotic Stresses

Transcriptome analysis reveals regulatory mechanisms of different drought-tolerant Gleditsia sinensis seedlings under drought stress

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