Isolation and characterization of <i>Actinopolyspora halophila</i>, gen. et sp. nov., an extremely halophilic actinomycete

Gochnauer, M. B.; Leppard, Gary G.; Komaratat, Prayad; Kates, Morris; Novitsky, Thomas J.; Kushner, D. J.

doi:10.1139/m75-222

Cited by 88 publications

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“…The occurrence of actinomycetes in highly saline environments is not rare (Gottlieb, 1973 ;Tresner et al, 1968). Members of the genus Actinopolyspora are either extreme or moderate halophiles (Gochnauer et al, 1975 ;Ruan et al, 1994 ;Yoshida et al, 1991). Among Nocardiopsis species, N. halophila is the only species that grows optimally in the presence of 5-15 % NaCl ; the trait defines the organism as a moderate halophile .…”

Section: Resultsmentioning

confidence: 99%

Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern.

Chun¹,

Bae²,

Moon³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

146

120

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A moderately halophilic actinomycete, designated HA-9 T , was isolated from a saltern in Kunsan, Republic of Korea, and was the subject of polyphasic identification. Analysis of 16S rDNA indicated that the isolate belonged to the genus Nocardiopsis, but differed genetically from other Nocardiopsis species. Strain HA-9T contained meso-diaminopimelic acid, no diagnostic sugars, hexaor octa-hydrogenated menaquinones with 10 isoprene units, straight-chain saturated or monounsaturated, iso-, anteiso-, 10-methyl branched fatty acids with 13-18 carbons and type III phospholipids. All of these characters consistently assign the isolate to the genus Nocardiopsis. All of the validly described Nocardiopsis species, including moderately halophilic Nocardiopsis halophila, can be differentiated from the saltern isolate using morphological and physiological traits. On the basis of polyphasic evidence, the name Nocardiopsis kunsanensis sp. nov. is proposed for strain HA-9 T (l KCTC 9831 T ), which is designated the type strain.Keywords : Nocardiopsis kunsanensis sp. nov., polyphasic taxonomy, 16S rDNA sequencing INTRODUCTIONThe genus Nocardiopsis was created by Meyer (1976) to harbour Actinomadura dassonvillei on the basis of morphological and chemotaxonomic properties. The genus currently comprises seven validly described species, namely Nocardiopsis alba, Nocardiopsis dassonvillei, Nocardiopsis halophila, Nocardiopsis listeri, Nocardiopsis lucentensis, Nocardiopsis prasina and Nocardiopsis synnemataformans Kroppenstedt, 1992 ;Yassin et al., 1993Yassin et al., , 1997. The members of Nocardiopsis are phylogenetically coherent and form a monophyletic clade that is equated with the family Nocardiopsaceae (Rainey et al., 1996). None of the Nocardiopsis species to date have been reported as halophilic except N. halophila, which can grow best at 5-15 % NaCl . In this study, we report the results of polyphasic identification of an actinomycete strain, designated HA-9 T , isolated from a saltern sample. optimally in medium containing 10 % NaCl and showed properties consistent with its classification in the genus Nocardiopsis Meyer 1976 as a new species, of which the name Nocardiopsis kunsanensis sp. nov. is proposed. METHODSBacterial strains. The saltern sample was collected from Kunsan, Republic of Korea. Bacterial strains were isolated by plating serially diluted samples onto Bennett medium based on seawater (2 g glucose, 1 g yeast extract, 1 g malt extract, 2 g peptone, 20 g agar, 1 l aged seawater, pH 7n2). After incubating at 30 mC for 2 weeks, a visible colony, designated HA-9 T , was transferred and subcultured until pure culture was obtained. Complex medium (CM ; 7n5 g Casamino acids, 10 g yeast extract, 3 g sodium citrate, 10 g magnesium sulfate, 2 g potassium chloride, 1 ml 4n98 % iron sulfate, 100 g NaCl, 1 l distilled water, pH 7n4) was used for growing strain HA-9 T and Nocardiopsis halophila KCTC 9825 T (l A.S.4.1195). The strain was maintained as a glycerol suspension (20 %, w\v) at k80 mC. J. Chun and others colonies, ...

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Section: Resultsmentioning

confidence: 99%

Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern.

Chun¹,

Bae²,

Moon³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

146

120

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“…At the time of writing, the genus Actinopolyspora consists of five species with validly published names, namely Actinopolyspora halophila (the type species; Gochnauer et al, 1975), Actinopolyspora mortivallis (Yoshida et al, 1991), Actinopolyspora xinjiangensis (Guan et al, 2010a), Actinopolyspora alba and Actinopolyspora. erythraea (Tang et al, 2011).…”

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confidence: 99%

Actinopolyspora lacussalsi sp. nov., an extremely halophilic actinomycete isolated from a salt lake

Guan

Wei

Zhang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel halophilic, filamentous actinobacterium, designated strain TRM 40139 T , was isolated from a hypersaline habitat in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation of the genus Actinopolyspora and the organism was related most closely to the type strains of Actinopolyspora alba (97.6 % similarity), Actinopolyspora xinjiangensis (97.6 %) and Actinopolyspora erythraea (97.1 %). However, it had relatively lower mean DNA-DNA relatedness values with the above strains (36.4, 31.3 and 26.1 %, respectively). Optimal growth occurred at 35 6C, at pH 7.0 and in the presence of 12 % (w/v) NaCl. The whole-cell sugar pattern consisted of xylose, glucose, ribose and arabinose. The major fatty acids were iso-C 16 : 0 (28.0 %) and anteiso-C 17 : 0 (27.6 %). The diagnostic phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown phospholipids. The predominant menaquinones were MK-9(H 4 ) (49.8 %) and MK-10(H 4 ) (24.2 %). The G+C content of the genomic DNA was 66.4 mol%. Strain TRM 40139 T therefore represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora lacussalsi sp. nov. is proposed. The type strain is TRM 40139 T (5KCTC 19657 T 5CCTCC AA 2012020 T ).The genus Actinopolyspora, which belongs to the family Actinopolysporaceae (Zhi et al., 2009), was originally established by Gochnauer et al., (1975) and its description has been emended recently by Tang et al. (2011). At the time of writing, the genus Actinopolyspora consists of five species with validly published names, namely Actinopolyspora halophila (the type species; Gochnauer et al., 1975), Actinopolyspora mortivallis (Yoshida et al., 1991), Actinopolyspora xinjiangensis (Guan et al., 2010a), Actinopolyspora alba and Actinopolyspora. erythraea (Tang et al., 2011). Actinopolyspora iraqiensis (Ruan et al., 1994) has been reclassified as a later heterotypic synonym of Saccharomonospora halophila (Tang et al., 2011). The typical chemotaxonomic characteristics of the genus Actinopolyspora are type IV cell wall, phospholipid pattern type PIII, mycolic acids absent, MK-9 (H 4 ), MK-10 (H 4 ) and MK-6 as predominant menaquinones, and iso-C 15 : 0 , iso-C 16 : 0 , iso-C 17 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 as major fatty acids (Gochnauer et al., 1975;Yoshida et al., 1991;Guan et al., 2010a;Tang et al., 2011). In this paper, we described a novel Actinopolyspora-like strain, designated TRM 40139 T . The aim of this study was to identify the exact taxonomic status of strain TRM 40139 T by using a polyphasic taxonomic approach.Strain TRM 40139 T was isolated from a soil sample collected from the Lop Nur salt lake, Xinjiang Province, north-west China (GPS coordinates for the sampling site 39 u 419 790 N 89 u 549 500 E), by using GL3 medium. The lake en...

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“…The family currently consists of 14 genera with validly published names: Actinoalloteichus (Tamura et al, 2000), Actinopolyspora (Gochnauer et al, 1975), Amycolatopsis (Lechevalier et al, 1986), Crossiella (Labeda, 2001), Goodfellowia (Labeda & Kroppenstedt, 2006), Kibdelosporangium (Shearer et al, 1986), Kutzneria (Stackebrandt et al, 1994), Prauserella (Kim & Goodfellow, 1999), Pseudonocardia (Henssen, 1957), Saccharomonospora (Nonomura & Ohara, 1971), Saccharopolyspora (Lacey & Goodfellow, 1975), Streptoalloteichus (Tomita et al, 1987), Thermobispora (Wang et al, 1996) and Thermocrispum (Korn-Wendisch et al, 1995). Strain YIM 0006 T was isolated during an investigation of actinomycete diversity in soil from a tropical rainforest in Chiang Mai, in northern Thailand.…”

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confidence: 99%

Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Jiang

Wiese

Tang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel actinomycete strain, YIM 0006 T , was isolated from soil of a tropical rainforest in northern Thailand. The isolate displayed the following characteristics: aerial mycelium is absent, short spore chains are formed directly on the substrate mycelium, contains meso-diaminopimelic acid, arabinose and galactose (cell-wall chemotype IV), the diagnostic phospholipid is phosphatidylcholine, MK-9(H 4 ) is the predominant menaquinone and the G+C content of the genomic DNA is 69.0 mol%. Phylogenetic analysis and phenotypic characteristics showed that strain YIM 0006 T belongs to the family Pseudonocardiaceae but can be distinguished from representatives of all genera classified in the family. The novel genus and species Actinomycetospora chiangmaiensis gen. nov., sp. nov. are proposed, with strain YIM 0006 T (5CCTCC AA 205017 T 5DSM 45062 T ) as the type strain of Actinomycetospora chiangmaiensis.The first description of the family Pseudonocardiaceae was given by Embley et al. (1988), and the description was emended by Stackebrandt et al. (1997) et al., 1996) and Thermocrispum (Korn-Wendisch et al., 1995). Strain YIM 0006 T was isolated during an investigation of actinomycete diversity in soil from a tropical rainforest in Chiang Mai, in northern Thailand. Here, we report on the classification and characterization of strain YIM 0006 T and propose a novel genus and species of the family Pseudonocardiaceae to accommodate the strain.Strain YIM 0006 T was isolated from a soil sample after 2 weeks incubation at 28 u C on starch-glycerol medium as described by Jiang et al. (2006). Cultural characteristics of the strain were determined after growth at 28 u C for 2 weeks by methods used in the International Streptomyces Project (ISP; Shirling & Gottlieb, 1966) as well as by using Czapek's medium and nutrient agar (Dong & Cai, 2001). Colour determination was performed with colour chips from the ISCC-NBS Color Charts Standard Samples no. 2106 (Kelly, 1964). Morphological observations of spore chains and mycelia were made by light microscopy (Olympus microscope BH-2) and scanning electron microscopy (Philips XL30 ESEM-TMP) after 20-50 days incubation. Gram staining (Hucker's modification; Society for American Bacteriologists, 1957) and Ziehl-Neelsen preparations (Gordon, 1967) were evaluated by light microscopy.Growth of strain YIM 0006 T was poor on most media tested, although the strain grew well but slowly on yeast extractmalt extract agar (ISP 2). Soluble pigments were not produced under any conditions tested in this study. Aerial mycelium was not observed on any of the tested media. The vegetative mycelium fragmented into rod-shaped elements (Fig. 1a) and was pale to brilliant orange-yellow in colour. Short spore chains were formed directly on the vegetative mycelium (Fig. 1a, b). The strain displayed bud-like structures of the spore chains, as has also been described for members of the genus Pseudonocardia (Huang et al., 2002). Spores were short and rod-shaped, 0.3-0.660.8-1.2 mm. The spore surface was smooth.The...

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Isolation and characterization of Actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete

Cited by 88 publications

References 0 publications

Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern.

Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern.

Actinopolyspora lacussalsi sp. nov., an extremely halophilic actinomycete isolated from a salt lake

Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

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