Isolation and characterization of human mesenchymal stem cells from gingival connective tissue

Jin, Seong-Ho; Lee, Je; Yun, Jin-Mun; I, Kim; Y, Ko; Jb, Park

doi:10.1111/jre.12228

Cited by 121 publications

(137 citation statements)

References 33 publications

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“…Conversely, GMSC cultured in adipogenic medium express PPARγ-2 [7] and form lipid globules characteristic of adipogenic differentiation [57] . In comparison to other CMF sources of MSC, GMSC undergo a greater degree of adipogenesis as compared to PDLSC, but less so than DPSC [56] . GMSC also exhibit the capability to differentiate towards the chondrogenic lineage, as evidenced by strong staining for the chondrogenic markers aggrecan and collagen type II α1 [56] .…”

Section: Profile and Identitymentioning

confidence: 82%

“…In comparison to other CMF sources of MSC, GMSC undergo a greater degree of adipogenesis as compared to PDLSC, but less so than DPSC [56] . GMSC also exhibit the capability to differentiate towards the chondrogenic lineage, as evidenced by strong staining for the chondrogenic markers aggrecan and collagen type II α1 [56] . Such successfully differentiated progenitor cells derived from gingival connective tissue exhibit increased proteoglycan production [57] .…”

Section: Profile and Identitymentioning

confidence: 82%

“…GMSC express high levels of the MSC surface markers CD13, CD44, CD73, CD90, CD105, STRO-1 and SSEA-4, but do not express hematopoietic markers CD14, CD19, CD34, CD38, CD45 and CD54 [56][57][58] . With their ease of accessibility from clinically resected gingival tissues during routine dental procedures, GMSC are an attractive alternative to other MSC [7,33] .…”

Section: Profile and Identitymentioning

confidence: 99%

“…GMSC are multipotent and can differentiate towards the osteogenic, adipogenic and chondrogenic lineages. Culturing of GMSC in osteoinductive medium leads to mineralization of extracellular deposits [56] and formation of calcium deposits [57] .…”

Section: Profile and Identitymentioning

confidence: 99%

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Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

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Tissue specific mesenchymal stem cells are of great scientific interest across diverse fields in stem cell biology and medicine. Sources of mesenchymal stem cells (MSC) in the craniomaxillofacial skeleton are numerous and are of significant interest to craniofacial and oral surgeons, among other medical and dental specialties. Mesenchymal stem cells are defined by their characteristic cell surface marker expression, multipotentiality and ability for self-renewal. Bone marrow derived MSC (BMSC) are most commonly studied, but are of low abundance in the craniomaxillofacial (CMF) skeleton. Alternative CMF sources of MSC are diverse and include dental pulpal stem cells (DPSC), periodontal ligament stem cells (PDLSC), suture-derived mesenchymal stromal cells (SMC), gingival mesenchymal stem cells (GMSC)and adipose-derived MSC (ASC). This brief review will introduce various MSC sources derived from the head and neck, along with a discussion of their identity, characteristics and results of preclinical studies in tissue engineering.

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Section: Profile and Identitymentioning

confidence: 82%

Section: Profile and Identitymentioning

confidence: 82%

Section: Profile and Identitymentioning

confidence: 99%

Section: Profile and Identitymentioning

confidence: 99%

See 2 more Smart Citations

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

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“…Cell numbers were determined by triplicate counting using a Casy® TTC cell counter. For all samples, the data is shown as mean ± SD (*p < 0.05) dental pulp [11,12], or from less specialized intra-oral connective tissue compartments, such as the gingiva [13,15,[31][32][33][34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies

et al. 2016

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A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva

Abedian

Jenabian

Moghadamnia

et al. 2020

Journal of Periodontology

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Background Mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL) and gingiva can be used for the development of cell‐based regenerative approaches in dentistry and medicine. The purpose of this investigation was to establish a method for isolation of human stem cells from the PDL and gingiva, multilineage differentiation of those cells, and comparison of periodontal ligament mesenchymal stem cells (PDLMSCs) and gingival mesenchymal stem cells (GMSCs). Methods PDL and gingival tissues of third molar teeth were digested enzymatically and the proliferative potential of human PDLMSCs and GMSCs was compared by MTT assay. The expression of cell surface epitopes was analyzed by flow cytometry. To investigate the multilineage differentiation capacity of these stem cells, osteogenic and adipogenic differentiation was achieved. The specific staining of nodules was performed to evaluate differentiation, whereas the expression of alkalin phosphatase (ALP) and collagen A I (COL I) genes was analyzed by quantitative real‐time polymerase chain reaction. Results The outgrown cells derived from PDL and gingival tissues were similar, fibroblast‐like, and spindle‐shaped. Further, the proliferation potential of GMSCs was greater than PDLMSCs. Both types of stem cells expressed MSC precursor markers, including CD73, CD90, and CD105, whereas they were negative for hematopoietic markers, including CD34 and CD45. PDLMSCs demonstrated more osteogenic potential compared to GMSCs with strong mineral noduls, and significantly greater expression of up‐regulated bone‐related markers ALP and COL I. Conclusion MSCs derived from PDL and gingiva demonstrated multipotent characteristics, suggesting new therapeutic approaches in tissue engineering and PDLMSCs are more appropriate candidates for this purpose.

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Isolation and characterization of human mesenchymal stem cells from gingival connective tissue

Cited by 121 publications

References 33 publications

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies

A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva

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