Isolation and characterization of fructophilic lactic acid bacteria from fructose-rich niches

Endo, Akihito; Futagawa-Endo, Yuka; Dicks, Leon M. T.

doi:10.1016/j.syapm.2009.08.002

Cited by 157 publications

(206 citation statements)

References 22 publications

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“…Lactic acid is strong enough to dissolve calcium carbonate and that can be seen as a clear area in the agar. Thus, in the isolation process clear areas indicate lactic acid bacteria [28]. Bifidobacteria population was determined on MRS agar supplemented with 0.05% of L-cysteine-HCl and 0.2% of bile salts [29].…”

Section: Animals Experimentalmentioning

confidence: 99%

Profile of Bacteria and Short Chain Fatty Acids of Caecal Digesta in Malnourished Rat Fed Goat Milk Yoghurt

Nurliyani¹,

Kandarina²,

Kusuma³

et al. 2014

JFNR

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The intestinal microbiota is an important determinant for general health of the human body, and disturbance of the proper balance of microbiota is involved in several pathologies. The profile of gastrointestinal microbiota can be influenced by nutritional factors and or health status of individuals. This study aimed to determine the effect of goat milk yogurt on the profile of caecal bacteria and short chain fatty acid (SCFA) in malnourished rats. Yoghurt was prepared by using of pasteurized goat milk with adding the Lacto-B powder containing Lactobacillus acidophilus, Sterptococcus thermophilus and Bifidobacterium longum. Male malnourished Wistar rats 3 weeks old were created using standard feed restriction up to 50% of normal rats for 21 d. After 21 d, the rats continued to restricted feeding and supplemented with goat milk yoghurt for 7 d. The rats were killed and analyzed the profile of caecal bacteria and SCFA. There were no significantly differences in the lactic acid bacteria (LAB) and bifidobacteria in both of caecal digesta of malnourished or normal rats fed yoghurt and control rats. However, the amount of E. coli was higher in malnourished rats than the normal rats. The acetic acid of caecal digesta was lower in the rats fed goat milk yoghurt than the control rats, whereas the butyric acid was higher in the caecal digesta of normal rats compared to the malnourished rats. The pH and moisture of caecal digesta in rats fed yoghurt were not significantly different from the control. In conclusion, goat milk yoghurt supplemented up to 2.0 ml/100 g body weight for 7 d had no effect on profile of caecal bacteria and physical properties and could not increase of caecal SCFA in malnourished and normal rats. Malnourished condition could increase the number of E. coli, decrease the butyric acid and weight of caecal.

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Section: Animals Experimentalmentioning

confidence: 99%

Profile of Bacteria and Short Chain Fatty Acids of Caecal Digesta in Malnourished Rat Fed Goat Milk Yoghurt

Nurliyani¹,

Kandarina²,

Kusuma³

et al. 2014

JFNR

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“…Catalase activity was not detected from the three strains grown in FYP broth under aerobic conditions but was detected when the novel strains were cultured on FYP agar containing 5 % (v/v) sheep blood under aerobic conditions. The strains produced DL-lactic acid, ethanol and acetic acid at a ratio of 1 : 0.8 : 0.2 (Endo et al, 2009) Growth of the three strains was slow on D-glucose and fast on D-fructose (Endo et al, 2009). Growth on D-glucose was enhanced in the presence of 1 % (w/v) pyruvate or under aerobic conditions, suggesting that an electron acceptor enhanced the growth of the novel strains.…”

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confidence: 96%

“…Pyruvate and oxygen are used as electron acceptors. At the time of writing, only five species with these characteristics have been described, Fructobacillus fructosus, Fructobacillus durionis, Fructobacillus ficulneus and Fructobacillus pseudoficulneus (Endo & Okada, 2008) and Lactobacillus kunkeei (Endo et al, 2009).During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al, 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics.…”

mentioning

confidence: 99%

“…During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al, 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics.…”

mentioning

confidence: 99%

“…The type strain is F9-1 T (5JCM 16035 T 5DSM 22689 T 5NRIC 0771 T ). The type strain, F9-1 T (5JCM 16035 T 5DSM 22689 T 5 NRIC 0771 T ), was isolated from peony and bietou flowers (Endo et al, 2009) Back et al, 1996); 3, L. sanfranciscensis (Kandler & Weiss, 1986;Hammes & Hertel, 2006); 4, L. fructivorans (Kandler & Weiss, 1986); 5, L. homohiochii (Kandler & Weiss, 1986;Hammes & Hertel, 2006 …”

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Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Endo

Futagawa-Endo

Sakamoto

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Three strains of fructophilic lactic acid bacteria were isolated from flowers in South Africa. The isolates formed a subcluster in the Lactobacillus buchneri phylogenetic group, closely related to Lactobacillus fructivorans, Lactobacillus homohiochii, Lactobacillus lindneri and Lactobacillus sanfranciscensis according to phylogenetic analysis based on the 16S rRNA gene sequences. Levels of DNA-DNA relatedness indicated that the three strains belonged to the same taxon and formed a genetically distinct group, well separated from their phylogenetic relatives. The three strains produced acids from only two of the 49 carbohydrates tested, i.e. D-glucose and Dfructose. D-Fructose was more rapidly fermented than D-glucose. Good growth was recorded on D-fructose or D-glucose in the presence of external electron acceptors. However, delayed growth was recorded on D-glucose without electron acceptors. The novel strains produced lactic acid, ethanol and acetic acid from D-glucose at a ratio of 1 : 0.8 : 0.2. These characteristics were distinct from other species of the genus Lactobacillus. Based on the data provided, the three isolates represent a fructophilic and novel species of the genus Lactobacillus, for which the name Lactobacillus florum sp. nov. is proposed. The type strain is F9-1 T (5JCM 16035 T 5DSM 22689 T 5NRIC 0771 T ).Fructophilic lactic acid bacteria (LAB) are a group of LAB described recently (Endo & Okada, 2008). These organisms are known to have unique characteristics, i.e. they grow well on D-fructose or D-glucose in the presence of external electron acceptors, but grow poorly on D-glucose without electron acceptors (Endo & Okada, 2008). Pyruvate and oxygen are used as electron acceptors. At the time of writing, only five species with these characteristics have been described, Fructobacillus fructosus, Fructobacillus durionis, Fructobacillus ficulneus and Fructobacillus pseudoficulneus (Endo & Okada, 2008) and Lactobacillus kunkeei (Endo et al., 2009).During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al., 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics. They formed a subcluster within the Lactobacillus buchneri cluster on phylogenetic analysis based on their 16S rRNA gene sequences and were closely related to Lactobacillus lindneri. Levels of DNA2DNA similarity and biochemical characteristics showed clear separation of the isolates from L. lindneri. This paper describes the taxonomic study of the three novel isolates and their classification as a novel species of the genus Lactobacillus. The three novel strains, F9-1 T , F9-2 and F17, grew well in FYP broth, but poorly in GYP broth and were thus regarded as fructophilic LAB. They were preserved at 280 u C in nutrient broth containing 20 % (v/v) glycerol.The 16S rRNA gene sequences of the three isolates were determined according to a previously described method (Endo & Okada, 2005). The closest recognized relatives of t...

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Physiology of the LAB

Endo

Dicks

2014

Lactic Acid Bacteria

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Isolation and characterization of fructophilic lactic acid bacteria from fructose-rich niches

Cited by 157 publications

References 22 publications

Profile of Bacteria and Short Chain Fatty Acids of Caecal Digesta in Malnourished Rat Fed Goat Milk Yoghurt

Profile of Bacteria and Short Chain Fatty Acids of Caecal Digesta in Malnourished Rat Fed Goat Milk Yoghurt

Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Physiology of the LAB

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