Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Endo, Akihito; Futagawa-Endo, Yuka; Sakamoto, Mitsuo; Kitahara, Maki; Dicks, Leon M. T.

doi:10.1099/ijs.0.019067-0

Cited by 69 publications

(54 citation statements)

References 17 publications

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“…These M. smithiiassociated OTUs were found in both fungus-garden and worker samples (see Table S2 in the supplemental material). As already reported in McFrederick et al (16), halictids associated mainly with Lactobacillus OTUs that were related to flower-inhabiting, fructophilic lactobacilli (52) or to a clade that includes flower-inhabiting and fructophilic lactobacilli (53) and lactobacilli used in sourdough fermentation (54). Some of the halictidassociated OTUs formed monophyletic clades, but these clades did not exhibit the same level of divergence as the Firm3 and Firm4 clades (Fig.…”

Section: Resultsmentioning

confidence: 55%

Specificity between Lactobacilli and Hymenopteran Hosts Is the Exception Rather than the Rule

McFrederick

Cannone

Gutell

et al. 2013

Appl Environ Microbiol

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c Lactobacilli (Lactobacillales: Lactobacillaceae) are well known for their roles in food fermentation, as probiotics, and in human health, but they can also be dominant members of the microbiota of some species of Hymenoptera (ants, bees, and wasps). Honey bees and bumble bees associate with host-specific lactobacilli, and some evidence suggests that these lactobacilli are important for bee health. Social transmission helps maintain associations between these bees and their respective microbiota. To determine whether lactobacilli associated with social hymenopteran hosts are generally host specific, we gathered publicly available Lactobacillus 16S rRNA gene sequences, along with Lactobacillus sequences from 454 pyrosequencing surveys of six other hymenopteran species (three sweat bees and three ants). We determined the comparative secondary structural models of 16S rRNA, which allowed us to accurately align the entire 16S rRNA gene, including fast-evolving regions. BLAST searches and maximum-likelihood phylogenetic reconstructions confirmed that honey and bumble bees have host-specific Lactobacillus associates. Regardless of colony size or within-colony oral sharing of food (trophallaxis), sweat bees and ants associate with lactobacilli that are closely related to those found in vertebrate hosts or in diverse environments. Why honey and bumble bees associate with host-specific lactobacilli while other social Hymenoptera do not remains an open question. Lactobacilli are known to inhibit the growth of other microbes and can be beneficial whether they are coevolved with their host or are recruited by the host from environmental sources through mechanisms of partner choice.

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Section: Resultsmentioning

confidence: 55%

Specificity between Lactobacilli and Hymenopteran Hosts Is the Exception Rather than the Rule

McFrederick

Cannone

Gutell

et al. 2013

Appl Environ Microbiol

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“…Fructobacillus spp. and L. kunkeei are representatives of these microorganisms, and a few novel species have recently been classified as members of this interesting group [84,85].…”

Section: Honey Analysis 240mentioning

confidence: 99%

Microorganisms in Honey

Silva¹,

Rabadzhiev²,

Eller³

et al. 2017

Honey Analysis

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Honey is a product with low water activity because of the great amount of sugars (fructose and glucose), and also it has antimicrobial compounds derived from flowers or because of its transformation process in the beehive. Despite all the honey microorganism barriers, some species of microorganisms are able to survive and may cause damage to honeybees or consumers. Techniques of pathogenic microorganism identification by DNA using PCR are recommended and required for sanitary and customs control. It is important to know the diversity of contaminating microorganisms in honey, especially due to disseminate pathogenic microorganisms in the international traded marketing. In contrast, beneficial microorganisms such as yeasts can remain latently in this product waiting for the moment in which the environment is suitable for their development. Among the beneficial bacteria found in honeybee products, we can mention some lactic acid bacteria that act as prebiotics when ingested. The microorganisms in the digestive tract of honeybees are important for their health. Thus, we present the knowledge of microbiota associated with honey from honeybees and stingless bees (Hymenoptera, Apidae) and the techniques available for the detection of microorganisms in honey.

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“…The low synthesis of ethanol is due to the absence of acetaldehyde dehydrogenase and a weak activity of alcohol dehydrogenase (7). The second group includes Lactobacillus florum and a few biotypes of Lactobacillus brevis (2,4,5,27). Facultative FLAB grow well on fructose and are able to grow on glucose at a delayed rate if electron acceptors are absent.…”

mentioning

confidence: 99%

“…ructophilic lactic acid bacteria (FLAB), described only recently (1)(2)(3)(4)(5)(6)(7)(8)(9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4,10,11).…”

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confidence: 99%

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

Filannino

Cagno

Addante

et al. 2016

Appl Environ Microbiol

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Fructophilic lactic acid bacteria (FLAB) are strongly associated with the gastrointestinal tracts (GITs) of Apis mellifera L. worker bees due to the consumption of fructose as a major carbohydrate. Seventy-seven presumptive lactic acid bacteria (LAB) were isolated from GITs of healthy A. mellifera L. adults, which were collected from 5 different geographical locations of the Apulia region of Italy. Almost all of the isolates showed fructophilic tendencies: these isolates were identified as Lactobacillus kunkeei (69%) or Fructobacillus fructosus (31%). A high-throughput phenotypic microarray targeting 190 carbon sources was used to determine that 83 compounds were differentially consumed. Phenotyping grouped the strains into two clusters, reflecting growth performance. The utilization of phenolic acids, such as p-coumaric, caffeic, syringic, or gallic acids, as electron acceptors was investigated in fructose-based medium. Almost all FLAB strains showed tolerance to high phenolic acid concentrations. p-Coumaric acid and caffeic acid were consumed by all FLAB strains through reductases or decarboxylases. Syringic and gallic acids were partially metabolized. The data collected suggest that FLAB require external electron acceptors to regenerate NADH. The use of phenolic acids as external electron acceptors by the 4 FLAB showing the highest phenolic acid reductase activity was investigated in glucose-based medium supplemented with p-coumaric acid. Metabolic responses observed through a phenotypic microarray suggested that FLAB may use p-coumaric acid as an external electron acceptor, enhancing glucose dissimilation but less efficiently than other external acceptors such as fructose or pyruvic acid. Fructophilic lactic acid bacteria (FLAB), described only recently (1-9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4, 10, 11). Among these insects, social honeybees are those of greater interest because of their economic and ecological importance for honeybee products (e.g., propolis, royal jelly, honey, and pollen) and especially for crop pollination. Despite a global increase in the population of domesticated bees according to the FAO data (12), honeybees are facing growing adversity. Localized declines in bee populations have occurred in many European countries (13-15). To understand and to prevent the decrease, several studies have been addressed to investigate the symbiotic and pathogenic microbial interactions (16-19). The observations made as of to date indicate the honeybees' GITs harbor a core microbiota dissimilar to those of other animals, including humans (16)(17)(18)(19)(20)(21)(22)(23)(24). FLAB are strongly as...

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Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Cited by 69 publications

References 17 publications

Specificity between Lactobacilli and Hymenopteran Hosts Is the Exception Rather than the Rule

Specificity between Lactobacilli and Hymenopteran Hosts Is the Exception Rather than the Rule

Microorganisms in Honey

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

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