Isolation and Characterization of Flavonol Glycosides from Leaves Extract of Lupinus arboreus Sims

Abstract

We planned to isolate the active constituents from methanol extract of Lupinus arboreus leaves. The crude methanol extract (CME) was fractionated into hexane, ethyl acetate and methanol fractions. The fractions were isolated from silica gel column chromatography, which were eluted with gradient mixtures. The isolated fractions were characterized by using flavonoid screening, IR, UV and NMR spectral analyses. The result of the phytochemical analysis and chromatography of the ethyl acetate fraction over silica gel lead to the isolation of a flavonol glycosides, and identified as tetrahydroxyflavone-3α-rhamnoside. Bioactivityguided result indicated tetrahydroxyflavone-3α-rhamnoside and imparts anti-inflammatory compound in this plant.

Chemistry and Pharmacology of Flavonoids- A Review

“…The carbohydrates (D-glucose, L-rhamnose, glucorhamnose, galactose or arabinose) are formed via glycosidic linkage generally positioned at positions 3 or 7. 6,29…”

Lupin, a Unique Legume That Is Nodulated by Multiple Microsymbionts: The Role of Horizontal Gene Transfer

“…The high protein content of lupin seeds (up to 44% dry weight) is similar to that of soybean, and lupin has been proposed as a viable alternative to soybean cultivation in Europe [ 47 ]. Lupin seeds possess a high nutritional value and unique phytochemical profile and biological activities [ 48 , 49 ]. Among the lupin species, L. albus (white lupin), L. luteus (yellow lupin), L. angustifolius (blue lupin), and L. mutabilis (tarwi) have gained agricultural market importance and have become part of the modern agriculture and food systems for high-protein production [ 47 ].…”