Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol

Saber, D. L.; Crawford, Ronald L.

doi:10.1128/aem.50.6.1512-1518.1985

Cited by 275 publications

(80 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mechanisms for the aerobic degradation of chlorinated phenols containing more than three chlorine substituents differ from that of monoand dichlorophenols. Several aerobic bacteria, belonging to the genera Rhodoeoccus, Mycobacterium, Arthrobacter, Flavobacterium, Pseu- 33 domonas and Azotobacter, that degrade pentachlorophenol and/or other polychlorinated phenols have been isolated [36][37][38][39][40][41][42][43][44][45]. In several of the studies, mineralization of pentachlorophenol was demonstrated by using a =4C-labelled substrate or by measuring stoichiometric release of chloride.…”

Section: Aerobic Bacterial Degradation Of Halophenolsmentioning

confidence: 99%

Microbial breakdown of halogenated aromatic pesticides and related compounds

Häggblom

1992

FEMS Microbiology Letters

363

132

View full text Add to dashboard Cite

Considerable progress has been made in the last few years in understanding the mechanisms of microbial degradation of halogenated aromatic compounds. Much is already known about the degradation mechanisms under aerobic conditions, and metabolism under anaerobiosis has lately received increasing attention. The removal of the halogen substituent is a key step in degradation of halogenated aromatics. This may occur as an initial step via reductive, hydrolytic or oxygenolytic mechanisms, or after cleavage of the aromatic ring at a later stage of metabolism. In addition to degradation, several biotransformation reactions, such as methylation and polymerization, may take place and produce more toxic or recalcitrant metabolites. Studies with pure bacterial and fungal cultures have given detailed information on the biodegradation pathways of several halogenated aromatic compounds. Several of the key enzymes have been purified or studied in cell extracts, and there is an increasing understanding of the organization and regulation of the genes involved in haloaromatic degradation. This review will focus on the biodegradation and biotransformation pathways that have been established for halogenated phenols, phenoxyalkanoic acids, benzoic acids, benzenes, anilines and structurally related halogenated aromatic pesticides. There is a growing interest in developing microbiological methods for clean‐up of soil and water contaminated with halogenated aromatic compounds.

show abstract

Section: Aerobic Bacterial Degradation Of Halophenolsmentioning

confidence: 99%

Microbial breakdown of halogenated aromatic pesticides and related compounds

Häggblom

1992

FEMS Microbiology Letters

363

132

View full text Add to dashboard Cite

show abstract

“…Microorganisms are responsible for the biodegradation of waste material and toxic pollutants in natural environments and PCP degradation has been studied thoroughly in the soil bacterium Sphingomonas chlorophenolica , isolated from a PCP-contaminated site in Minnesota (Saber and Crawford, 1985). PCP 4-monooxygenase ( pcpB gene) converts PCP to tetrachlorohydroquinone (Xun and Orser, 1991).…”

Section: Introductionmentioning

confidence: 99%

Cultivation‐independent in situ molecular analysis of bacteria involved in degradation of pentachlorophenol in soil

Mahmood¹,

Paton²,

Prosser³

2005

Environmental Microbiology

View full text Add to dashboard Cite

The central aim of this study was to determine which components of an indigenous bacterial community in pristine grassland soil were capable of degrading pentachlorophenol (PCP) using two cultivation-independent, in situ, molecular techniques. The first involved polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) amplification of 16S rRNA genes from DNA and RNA, respectively, extracted from PCP-amended soil. The second involved stable isotope probing (SIP), with incubation of soil with 13C-PCP and molecular analysis of 13C-labelled RNA, derived from cells incorporating PCP or its breakdown products, after separation from 12C-RNA by ultracentrifugation. Bacterial communities were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of amplification products. PCP was degraded at an approximate rate of 1.18+/-0.25 (SEM) mg kg-1 day-1 and 39% of the measurable PCP fraction was degraded after incubation for 63 days. PCP degradation was associated with significant changes in bacterial community structure, leading to the appearance of seven bands in both DNA- and RNA-based DGGE profiles, the latter providing clearer evidence of qualitative shifts in community structure. The majority of novel bands increased in relative intensity during the first 35 days and subsequently decreased in relative intensity as incubation continued. Sequence and phylogenetic analysis of six of these bands indicated most to have closest database relatives that were uncultured bacteria with sequence homologies to reported hydrocarbon degraders. No band could be detected in RNA-SIP-DGGE profiles derived from 13C-RNA fractions at day 0 but several faint bands appeared in these fractions after incubation of soil for 4 days, indicating assimilation of PCP or its degradation products. These bands increased in intensity during subsequent incubation for 21 days and decreased with further incubation. With one exception, RNA-SIP-DGGE and RNA-DGGE profiles were similar, indicating that RNA-targeted DGGE, in this case, provided a good indication of the metabolically active microbial community.

show abstract

“…The presence of PCDDs and PCDFs is of particular concern in PCP solutions. PCP is acutely toxic to a variety of organisms and mammals, as an inhibitor of oxidative phosphorylation (Brummet & Ordal, 1977;Saber & Crawford, 1985;Shore et al, 19911, and PCP is a fat-soluble chemical accumulating in fish through the food chain (Renberg et al, 1983 PCP is slightly soluble in water ( 8 mg per 100 mL) but very soluble in oil. Consequently, it is generally dissolved in diesel oil for wood treatment; leaks and spills on the ground often collect as light nonaqueous phase liquids (LNAPLS) on top of the water table.…”

Section: Pentachlorophenol (Pcp)mentioning

confidence: 99%

Treatment technology for remediation of wood preserving sites: Overview

Bates

Grosse

Sahle‐Demessie

2000

Remediation Journal

View full text Add to dashboard Cite

This is the first in a series of five articles describing the applicability, performance, and cost of technologies for the remediation of contaminated soil and water at wood preserving sites. Site‐specific treatability studies conducted under the supervision of the United States Environmental Protection Agency (US EPA), National Risk Management Research Laboratory (NRMRL), from 1995 through 1997 constitute much of the basis for the evaluations presented, although data from other treatability studies, literature sources, and actual site remediations have also been included to provide a more comprehensive evaluation of remediation technologies. This article provides an overview of the wood preserving sites studied, including contaminant levels, and a summary of the performance of the technologies evaluated. The subsequent articles discuss the performance of each technology in more detail. Three articles discuss technologies for the treatment of soils, including solidification/stabilization, biological treatment, solvent extraction and soil washing. One article discusses technologies for the treatment of liquids, water and nonaqueous phase liquids (NAPLS), including biological treatment, carbon adsorption, photolytic oxidation, and hydraulic containment. The reader should be aware that other technologies including, but not limited to, incineration, thermal desorption, and base catalyzed dehalogenation, also have application for treating contaminants on wood preserving sites. They are not discussed in these five articles since the focus was to evaluate lesser known and hopefully lower cost approaches. However, the reader should include consideration of these other technologies as part of any evaluation or screening of technologies applicable to remediation of wood preserving sites.

show abstract

Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol

Cited by 275 publications

References 32 publications

Microbial breakdown of halogenated aromatic pesticides and related compounds

Microbial breakdown of halogenated aromatic pesticides and related compounds

Cultivation‐independent in situ molecular analysis of bacteria involved in degradation of pentachlorophenol in soil

Treatment technology for remediation of wood preserving sites: Overview

Contact Info

Product

Resources

About