Isolation and characterization of fibroblasts derived from regenerating human periodontal defects

Ivanovski, Sašo; Haase, H. R.; Bartold, P. Mark

doi:10.1016/s0003-9969(01)00036-x

Cited by 57 publications

(50 citation statements)

References 41 publications

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“…20 Also, compared to normal fibroblasts, healing fibroblasts produced a higher level of type I collagen in medium, expressed a greater level of α-SMA, and generated larger contraction forces. Intuitively, the results appear reasonable and are consistent with previous studies, because healing and normal fibroblasts have a different environment in vivo, and therefore they should function differently.…”

Section: Discussionmentioning

confidence: 94%

“…A previous study showed that fibroblasts derived from human periodontal ligaments from molar extractions were found to proliferate faster than those from an associated uninjured periodontal ligament following treatment with growth factors. 20 Also, while some degree of fibroblast contraction is necessary for wound closure, excessive fibroblast contraction can lead to the formation of scar tissue. 6,26 It has been suggested that during this period of excessive contraction, fibroblasts increase the expression of α-SMA.…”

Section: Introductionmentioning

confidence: 99%

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Healing and Normal Fibroblasts Exhibit Differential Proliferation, Collagen Production, α-SMA Expression, and Contraction

et al. 2006

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This study determines the differences in proliferation, collagen production, alpha-smooth muscle actin (alpha-SMA) expression, and contraction between healing and normal fibroblasts. Transected and sham-operated rat medial collateral ligaments (MCL) were used to obtain healing and normal fibroblasts, respectively. It was found that healing fibroblasts in monolayer culture proliferated 1.4-fold faster at 48 h and had 1.7-fold greater protein expression of alpha-SMA than normal fibroblasts. In addition, it was noted that the proliferation of healing fibroblasts in collagen gels was not significantly different from that of normal fibroblasts at 24 h, but it was at 48 h. Furthermore, in collagen gels, healing fibroblasts produced more type I collagen than normal fibroblasts and generated 1.6- and 1.7-fold larger contractile forces at 15 and 20 h, respectively, than their normal counterparts. Taken together, the results of this study show that healing fibroblasts possess a differential proliferation, alpha-SMA protein expression, and contraction than normal fibroblasts.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Healing and Normal Fibroblasts Exhibit Differential Proliferation, Collagen Production, α-SMA Expression, and Contraction

et al. 2006

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“…Evidently, the level of regeneration in the periodontal ligament corresponds to the second type. A recent study has demonstrated that fibroblasts obtained from regenerating periodontal defects exhibit characteristics consistent with their ability to facilitate periodontal regeneration (Ivanovski et al, 2001b).…”

Section: Regenerative Capability and Cell Proliferation Of Thementioning

confidence: 99%

Regulatory mechanisms of periodontal regeneration

Shimono¹,

Ishikawa²,

Ishikawa³

et al. 2003

Microscopy Res & Technique

201

162

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The periodontal ligament, located between the cementum and the alveolar bone, has a width ranging from 0.15 to 0.38 mm. Regeneration and homeostasis of the periodontal ligament are highly significant functions in relation to periodontal therapy, tooth transplantation or replantation, and orthodontic tooth movement. The purpose of this review is to discuss the regulatory mechanisms of regenerative and homeostatic functions in the periodontal ligament based on currently published studies and also on our own experimental data. We consider the capability of the ligament tissue to promote or to suppress calcification in connection with bone and cementum formation and the maintenance of the periodontal ligament space. Also discussed are the involvement of the periodontal ligament tissue in the regenerative ability, cell proliferation, growth and differentiation factors, extracellular matrix proteins, homeostatic phenomena, function of Malassez epithelial rests, tooth movement, or occlusal loading. Regulatory mechanisms for regeneration and homeostasis of the periodontal ligament are hypothetically proposed.

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“…Earlier studies have identified dividing cells emerging from wounded PDL as fibroblast-like heterogeneous cells, derived from the vicinity of blood vessels, but not of haematopoietic origin (Gould et al 1977(Gould et al , 1980Ivanovski et al 2001;Limeback et al 1983;McCulloch 1985). Undifferentiated paravascular progenitors are further suggested as the source of these fibroblasts (Gould 1983;McCulloch et al 1987).…”

Section: Pdl Stem Cellsmentioning

confidence: 99%

Stem cells and tooth tissue engineering

2007

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The notion that teeth contain stem cells is based on the well-known repairing ability of dentin after injury. Dental stem cells have been isolated according to their anatomical locations, colony-forming ability, expression of stem cell markers, and regeneration of pulp/dentin structures in vivo. These dental-derived stem cells are currently under increasing investigation as sources for tooth regeneration and repair. Further attempts with bone marrow mesenchymal stem cells and embryonic stem cells have demonstrated the possibility of creating teeth from non-dental stem cells by imitating embryonic development mechanisms. Although, as in tissue engineering of other organs, many challenges remain, stem-cell-based tissue engineering of teeth could be a choice for the replacement of missing teeth in the future.

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Isolation and characterization of fibroblasts derived from regenerating human periodontal defects

Cited by 57 publications

References 41 publications

Healing and Normal Fibroblasts Exhibit Differential Proliferation, Collagen Production, α-SMA Expression, and Contraction

Healing and Normal Fibroblasts Exhibit Differential Proliferation, Collagen Production, α-SMA Expression, and Contraction

Regulatory mechanisms of periodontal regeneration

Stem cells and tooth tissue engineering

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