Isolation and characterization of fenamiphos degrading bacteria

Cabrera, J.A.; Kurtz, Andreas; Sikora, R. A.; Schouten, Alexander

doi:10.1007/s10532-010-9362-z

Cited by 31 publications

(21 citation statements)

References 34 publications

(33 reference statements)

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“…The phylogenetic tree showed sister linage between sequences of xenobiotic degrading microorganisms. The same results were obtained by Cabrera et al (2010) who reported that fenamiphos degrading bacteria were more closely related than others, and suggested that nematicide degrading bacteria were grouped within a common microbial population which can be of great significance for the soil ecology.…”

Section: Discussionsupporting

confidence: 80%

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Saafan¹,

Azmy²,

Amin³

et al. 2016

Afr. J. Biotechnol.

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Pseudomonas aeruginosa and Pseudomonas mendocina degrading malathion were studied. Morphological, biochemical and 16S rRNA genes for bacterial identification were selected. Biodegradation of some organophosphorus compounds with the 2 bacterial isolates was determined by high performance liquid chromatography (HPLC). P. aeruginosa strain completely removed diazinon, malathion and fenitrothion, but not chlorpyrifos within 14 days. P. mendocina strain was not able to degrade malathion, diazinon and chlorpyrifos completely and no significant degradation for chlorpyrifos. The bacterial growth curve showed a steady increase in the two bacterial isolates masses during malathion degradation. The highest growth rates were with yeast extract, glucose and citrate for the 2 isolates, but not with phenol. Shaked high inoculum density with incubation at 30°C of malathion bacterial cultures were found to be the optimum conditions for malathion degradation. Bacterial culture extracts subjected to liquid chromatography/mass spectrometry (LC/MS) analysis revealed that the separated products were malathion monocarboxylic acid and malathion dicarboxylic acid. Molecular characterization of carboxylesterase enzyme revealed that carboxylesterase amino acid sequences of the 2 isolates showed high identity to other carboxylesterase enzymes of P. aeruginosa and P. mendocina, respectively. Phylogenetic analysis showed that P. aeruginosa was localized in a separate branch from other carboxylesterase producing Pseudomonas sp. So, it is suggested that this enzyme is a novel esterase enzyme. Use of pesticide-degrading microbial systems for removal of organophosphorus compounds from the contaminated sites requires an understanding of ecological requirements of degrading strains. The results provided an important insight into determining the bioremediation potential of both strains. But the mentioned bacteria cannot be the aim of bioremediation due to risk of public health hazard, hence these bacteria cannot be used in bioremediation but their purified enzymes could.

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Section: Discussionsupporting

confidence: 80%

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Saafan¹,

Azmy²,

Amin³

et al. 2016

Afr. J. Biotechnol.

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“…Organophosphorus pesticides are degraded by many bacteria isolated from soil. For example, a Brevibacterium sp., Microbacterium esteraromaticum and other bacteria, were able to hydrolyse the P-O-C bond of fenamiphos (ethyl 4-methylthio-m-tolyl isopropylphosphoramidate) (Megharaj et al 2003;Caceres et al 2009;Cabrera et al 2010). Rhodococcus and Bacillus strains were also able to rapidly degrade methyl isothiocyanate (Warton et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Abo‐Amer

2011

World J Microbiol Biotechnol

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Bacteria capable of degrading the pesticide, cadusafos, were isolated from agricultural soil using an enrichment method. In this way, five distinct cadusafos-degrading strains of Pseudomonas putidia were isolated, and were characterized using morphological and biochemical analysis, as well as 16S rRNA sequencing. Strain PC1 exhibited the greatest cadusafos degradation rate and was consequently selected for further investigation. Degradation of cadusafos by strain PC1 was rapid at 20 and 37°C, but was greatly reduced (~1.5-fold) by the presence of carbon sources. Strain PC1 was able to effectively degrade cadusafos in sterilized soil using low inoculum levels. The maximum degradation rate of cadusafos (V ( max )) was calculated as 1.1 mg l(-1 )day(-1), and its saturation constant (K ( s )) was determined as 2.5 mg l(-1). Bacteria such as strain PC1, that use cadusafos as a carbon source, could be employed for the bioremediation of sites contaminated with pesticides.

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“…They were maintained under laboratory conditions in the dark at 14±1ºC. The targeted amount of fenamiphos applied in the field ranges from 10 to 45 mg/kg soil which results in the use of 6-10 kg/ha under practical conditions depending on the crop [16,17]. It was reported that normal field application rates of fenamiphos can vary between 0.5 and 10 kg/ha, equivalent to 5 and 100 mg/kg soil [18,19].…”

Section: Methodsmentioning

confidence: 99%

Toxic Effect of Fenamiphos on the Earthworm, Eisenia fetida (Annelida: Oligochaeta)

Akat¹,

Arman²

2016

HJBC

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Ö Z E T S unulan bu çalışma, fenamiphosun toprak solucanı Eisenia fetida üzerine olumsuz etkilerini inceleyen ilk çalışmadır. Toprak solucanları fenamiphosun farklı derişimlerine (5, 10 ve 20 mg/kg) maruz bırakıldı. Toprak solucanları 96 saat sonra ötenazi edildi ve solucanların fiksasyonu yapıldı. Toprak solucanlarının vücut duvarlarındaki (epidermis, halkasal ve boyuna kaslar), klorojen hücrelerindeki ve bağırsak epitelindeki histolojik değişiklikler değerlendirildi. Histopatolojik değişiklikler düşük doz (5 mg/kg) uygulama grubuna göre orta (10 mg/kg) ve yüksek doz (20 mg/kg) uygulama gruplarında daha belirgindi. Fenamiphos maruziyetinden sonra elde ettiğimiz bulgulara göre derideki ve bağırsak sistemindeki dejenerasyon bu iki sistemin fonksiyonunu tam olarak yerine getirememesi sonucu toprak solucanlarının canlılıklarını sürdürmeleri üzerine olumsuz etkiye neden olabilir. Anahtar KelimelerFenamiphos, toprak solucanı, bağırsak, vücut duvarı. A B S T R A C TT he aim of the present study was to investigate for the first time adverse effect of fenamiphos on the earthworm, Eisenia fetida. Earthworms were exposed to different concentrations of fenamiphos (5, 10 and 20 mg/kg). After 96 h, earthworms were euthanized and fixed. Histological alterations in earthworm's body wall (epidermis, circular and longitudinal muscles), chloragogenous tissue and intestinal epithelium were assessed. Histopathological changes were more prominent in medium (10 mg/kg) and high-concentration (20 mg/ kg) groups than in the low-concentration (5 mg/kg) group. Degeneration in the intestine and integumentary system, as our results indicated after exposure to fenamiphos, are likely to interfere with the correct functioning of the intestine and integumentary system may affect earthworm survival.

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Isolation and characterization of fenamiphos degrading bacteria

Cited by 31 publications

References 34 publications

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Toxic Effect of Fenamiphos on the Earthworm, Eisenia fetida (Annelida: Oligochaeta)

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