Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Abstract

Bacteria capable of degrading the pesticide, cadusafos, were isolated from agricultural soil using an enrichment method. In this way, five distinct cadusafos-degrading strains of Pseudomonas putidia were isolated, and were characterized using morphological and biochemical analysis, as well as 16S rRNA sequencing. Strain PC1 exhibited the greatest cadusafos degradation rate and was consequently selected for further investigation. Degradation of cadusafos by strain PC1 was rapid at 20 and 37°C, but was greatly reduced (~1.5-fold) by the presence of carbon sources. Strain PC1 was able to effectively degrade cadusafos in sterilized soil using low inoculum levels. The maximum degradation rate of cadusafos (V ( max )) was calculated as 1.1 mg l(-1 )day(-1), and its saturation constant (K ( s )) was determined as 2.5 mg l(-1). Bacteria such as strain PC1, that use cadusafos as a carbon source, could be employed for the bioremediation of sites contaminated with pesticides.

Selective Isolation of a Gram Negative Carbamate Pesticide Degrading Bacterium From Brinjal Cultivated Soil

“…Such technique can provide lower cost and fewer disturbances and can become suited to the country's need. Psedomonas is known to be a versatile oraganism having the ability to degrade various kinds of pollutants which has been isolated from various agricultural lands (Jilani, 2013; Abo- Amer, 2012). In this study a strain identified as Pseudomonas showed its tolerance and degradation capability to a carbosulfan pesticide.…”

Role of Microorganisms in the Degradation of Organic Pollutants: A Review

“…However, the pesticide industry's effluent, sewage sludge, activated sludge, wastewater, natural waters, sediments, areas surrounding the pesticide industries are also rich sources of pesticide degrading microorganisms [21]. Abo-Amer [22] reported that Pseudomonas, sp. is the most efficient bacterial genus for the degradation of toxic compounds.…”

“…Moreover, the biotransformation of such chemical pollutants depends not only on the presence of microorganisms with appropriate degrading enzymes, but on a wide range of environmental paremeters [21] such as temperature, pH, redox potentials, nutrients, contact time, water activity, etc. However, some other aspects such as physiological, ecological, biochemical and molecular, play important roles in the microbial transformation of pollutants [26,27,22].…”

Biorremediación de organofosforados por hongos y bacterias en suelos agrícolas: revisión sistemática

“…De acuerdo con la literatura científica, otros organofosforados utilizados en la agricultura han sido degradados por algunos microorganismos, sin embargo, existen pocos reportes de estos hallazgos. Algunos de estos son profenofos (utilizado en cultivos de algodón para el control de plagas), triazofos (que posee un amplio espectro de actividad), cadusafos (insecticida de amplio espectro utilizado contra nematodos e insectos), etoprofos, isofenfos, isazofos, fentión y tetraclorvinfos (utilizado contra parásitos externos del ganado y aves de corral) (Ortiz y Sánchez 2010; Chanika et al 2011;Abo-Amer 2012).…”