Isolation and characterization of feline panleukopenia virus from a diarrheic monkey

Wang, Cuiling; Wang, Shujun; Feng, Hao; Zeng, Lin; Xia, Zhang; Zhang, Renzhou; Zou, Xiaohuan; Wang, Chengyu; Liu, Quan; Xia, Xianzhu

doi:10.1016/j.vetmic.2009.11.023

Cited by 25 publications

(25 citation statements)

References 21 publications

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“…These clinical samples had been used to investigate the prevalence of FBoV and FeAstV using conventional single PCR in a previous study CAT ACA TGG CAA ACA AAT AGA GCA 237 FPV-R TGT TTT AAA TGG CCC TTG TGT AGA FBoV NP1 FBoV-F AGA ACC RCC RAT CAC ART CCACT 465 FBoV-R TGG CRA CCG CYA GCA TTT CA FeAstV RdRp FeAstV-F GCG GAT TGG GCA TGG TTT AGA 645 FeAstV-R ACC CCT CGT TTG GAT CGT TACCT performed in our laboratory [24,25]. In the present study, these clinical samples were used to detect FPV using routine PCR as reported previously [23]. The positive rate was calculated for each virus, and the difference in the prevalence of each virus between diarrheic and healthy cats was evaluated by the two-tail chi square test using PASW Statistic 19.0.…”

Section: Application Of the Mpcr Assay On Clinical Samplesmentioning

confidence: 99%

Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus

Zhang

Niu

et al. 2019

Arch Virol

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A multiplex polymerase chain reaction (mPCR) assay was developed to detect and distinguish feline panleukopenia virus (FPV), feline bocavirus (FBoV) and feline astrovirus (FeAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of the three viruses and were used to specifically amplify targeted fragments of 237 bp from the VP2 gene of FPV, 465 bp from the NP1 gene of FBoV and 645 bp from the RdRp gene of FeAstV. The results showed that this mPCR assay was effective, because it could detect at least 2.25-4.04 × 10 4 copies of genomic DNA of the three viruses per μl, was highly specific, and had a good broad-spectrum ability to detect different genotypes of the targeted viruses. A total of 197 faecal samples that had been screened previously for FeAstV and FBoV were collected from domestic cats in northeast China and were tested for the three viruses using the newly developed mPCR assay. The total positive rate for these three viruses was 59.89% (118/197). From these samples, DNA from FPV, FBoV and FeAstV was detected in 73, 51 and 46 faecal samples, respectively. The mPCR testing results agreed with the routine PCR results with a coincidence rate of 100%. The results of this study show that this mPCR assay can simultaneously detect and differentiate FPV, FBoV and FeAstV and can be used as an easy, specific and efficient detection tool for clinical diagnosis and epidemiological investigation of these three viruses.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Application Of the Mpcr Assay On Clinical Samplesmentioning

confidence: 99%

Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus

Zhang

Niu

et al. 2019

Arch Virol

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“…Finally, the CPV-like phylogeny also contained individual sequences from a virus sampled from a masked palm civet (GenBank accession number EU441280) (X. Yan, H. Zhang, T. Chen, J. Zhao, X. Chai, and W. Wu, unpublished data), which was linked by a long branch to the intermediate raccoon group (although with weak support), and a virus associated with an outbreak in rhesus monkeys (GenBank accession FJ231389) (27) that falls at the base of the CPV-like part of the tree (again with weak support). Because these are single viral sequences, it is difficult to determine whether they simply represent transient spillover infections.…”

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confidence: 99%

Frequent Cross-Species Transmission of Parvoviruses among Diverse Carnivore Hosts

Allison

Köhler

Fox

et al. 2013

J Virol

130

140

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i Although parvoviruses are commonly described in domestic carnivores, little is known about their biodiversity in nondomestic species. A phylogenetic analysis of VP2 gene sequences from puma, coyote, gray wolf, bobcat, raccoon, and striped skunk revealed two major groups related to either feline panleukopenia virus ("FPV-like") or canine parvovirus ("CPV-like"). Crossspecies transmission was commonplace, with multiple introductions into each host species but, with the exception of raccoons, relatively little evidence for onward transmission in nondomestic species.

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“…The detection of kobuvirus was performed by RT-PCR using a pair of universal primer previously described (the primer sequences are shown in Table 1), UNIV-kobu-F/UNIV-kobu-R targeting 217-bp of partial 3D gene for all kobuviruses (Reuter et al 2009). These samples were also examined for other feline enteric viruses, including feline parvovirus (FPV) and feline bocavirus (FBoV), using PCR assays previously described (Takano et al 2016;Yang et al 2010). The amplified products were separated after electrophoresis on 1.5% agarose gels at 160 V for…”

Section: Detection Of Fekov and Other Feline Enteric Virusesmentioning

confidence: 99%

Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Niu

Wang

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TFeline kobuvirus (FeKoV), a novel picornavirus of the genus kobuvirus, was initially identified in the feces of cats with diarrhea in South Korea in 2013. To date, there is only one report of the circulation of kobuvirus in cats in southern China. To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. Kobuvirus was identified in 28 fecal samples with an overall prevalence of 14.2% (28/197) of which 20 samples were co-infected with feline parvovirus (FPV) and/or feline bocavirus (FBoV). Diarrhoeic cats had a higher kobuvirus prevalence (19.1%, 20/105) than asymptomatic cats (8.7%, 8/ 92). By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%-97.8% and 96.6%-100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. Furthermore, two complete FeKoV polyprotein genomes were successfully obtained from two positive samples and designated 16JZ0605 and 17CC0811, respectively. The two strains shared 92.9%-94.9% nucleotide identities and 96.8%-98.4% amino acid identities to FeKoV prototype strains. Phylogenetic analysis indicated that FeKoVs were clustered according to their geographical regions, albeit with limited sequences support. This study provides the first molecular evidence that FeKoV circulates in cats in northeast China, and these FeKoVs exhibit genetic diversity and unique evolutionary trend.

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Isolation and characterization of feline panleukopenia virus from a diarrheic monkey

Cited by 25 publications

References 21 publications

Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus

Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus

Frequent Cross-Species Transmission of Parvoviruses among Diverse Carnivore Hosts

Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

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