Isolation and characterization of different plant associated bacteria and their potential to degrade polychlorinated biphenyls

Ionescu, Marieta; Beranova, Katarina; Dudková, Vlasta; Kochánková, Lucie; Demnerova, Kateřina; Macek, Tomáš; Macková, Martina

doi:10.1016/j.ibiod.2009.03.009

Cited by 57 publications

(45 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…on May 9, 2018 by guest http://aem.asm.org/ radish rhizosphere was chosen as a source material in this study, as horseradish was previously mentioned as a suitable candidate for bioremediation of PCBs (29). The main task of this study was to evaluate the applicability of MALDI-TOF MS and the MALDI Biotyper database for the classification of environmental isolates potentially exploitable in bioremediation.…”

Section: Vol 77 2011 Maldi-tof Ms For Environmental Isolate Identifmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Uhlík

Strejcek

Junková

et al. 2011

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (PCB)-contaminated soil vegetated by horseradish. Isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The usage of MALDI Biotyper for the classification of isolates was evaluated and compared to 16S rRNA gene sequence analysis. A wide spectrum of bacteria was isolated, with Arthrobacter, Serratia, Rhodococcus, and Rhizobium being predominant. Arthrobacter isolates also represented the most diverse group. The use of MALDI Biotyper in many cases permitted the identification at the level of species, which was not achieved by 16S rRNA gene sequence analyses. However, some isolates had to be identified by 16S rRNA gene analyses if MALDI Biotyper-based identification was at the level of probable or not reliable identification, usually due to a lack of reference spectra included in the database. Overall, this study shows the possibility of using MALDI-TOF MS and MALDI Biotyper for the fast and relatively nonlaborious identification/classification of soil isolates. At the same time, it demonstrates the dominant role of employing 16S rRNA gene analyses for the identification of recently isolated strains that can later fill the gaps in the protein-based identification databases.Bacterial identification has always been a major challenge in all microbiological fields. Originally, all bacteria were identified according to their phenotypic characteristics after they had been isolated in pure culture. Only in the 1980s did scientists discover a huge discrepancy in the bacterial contents in aquatic and terrestrial habitats expressed as CFU after cultivation and direct microscopic enumeration (62). This discovery contributed highly to the boom in molecular biological methods of bacterial identification, which are independent of cultivation. These techniques have enabled scientists to discover and study actual bacterial diversity, which in most environments is orders of magnitude larger than culture-based techniques are able to reveal (40). However, characterization of bacteria after isolation still remains the most effective way of identifying bacterial properties.The necessity of fast and accurate identification of bacteria has been driven mostly by the needs of clinical and food microbiology, as these fields are directly connected with human health. Whereas tests based on biochemical traits are still important for the identification of pathogenic bacteria (64), they mainly fail with microbes isolated from environmental samples, as the diversity of microbes in these habitats is enormous (70). Environmental isolates are usually classified based on the primary structures of 16S rRNA genes (9). The process of DNA isolation, amplification of target genes, and sequencing is, however, rather time-consuming. Therefore, the introduction of mass spectrometry (MS) for the purposes of bacterial identification (13,24) represented an important ...

show abstract

Section: Vol 77 2011 Maldi-tof Ms For Environmental Isolate Identifmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Uhlík

Strejcek

Junková

et al. 2011

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several authors have confirmed positive effects of root exudates on increased microbial residency and activities in rhizosphere (Bais et al 2004;Ionescu et al 2009;Segura and Ramos 2012). These exudations are principal factors through which roots may regulate/alter the soil microbial community in their immediate vicinity, cope with herbivores, encourage beneficial symbioses, change the chemical and physical properties of the soil and inhibit growth of competing plant species (Nardi et al 2000;Uhlik et al 2012).…”

Section: Rhizosphere: Hot Spot For Pollutants Degradationmentioning

confidence: 92%

“…Several investigators have already shown that specific plants may selectively foster PCB-degrading bacteria (Ryslava et al 2003;Villacieros et al 2003) (Table 1). Ionescu et al 2009 screened four plants as Medicago sativa, Nicotiana tobacum, Salix caprea, Armoracia rusticana and Solanum nigrum to study the differential effect of plant species on microbial community. Authors have found that S. caprea and A. rusticana promoted the growth of potential PCB degraders better than N. tabacum and S. nigrum.…”

Section: Rhizosphere: Hot Spot For Pollutants Degradationmentioning

confidence: 99%

“…Thus, last decade has gained tremendous momentum in the field of phytoremediation, and this approach has now entered into active field of development (Macek et al 2000;Divya and Kumar 2011). Several plants including pine tree, alfalfa, flatpea, willow, canarygrass, deertongue, switchgrass, tall fescue, poplar, tobacco and mustard among others have been tested for their efficiencies to reduce PCBs in spiked soils (Ficko et al 2010;Ionescu et al 2009;Meggo et al 2013). Low bioavailability in soil often results in limited utilization of PCBs by plants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Secondary plant metabolites and root exudates: guiding tools for polychlorinated biphenyl biodegradation

Panwar

Jha

2014

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Synthetic organic compounds are hallmark of modern society. They are ubiquitous ranging from home, workplace to agriculture industry, which leads to their nonjudicious dispensing into environment. Unfortunately most of them, especially polychlorinated biphenyls (PCBs), are deemed as persistent organic pollutants posing serious health risks to human. Hence, there is an alarming need of phasing out these chemicals and remediating contaminated sites in eco-friendly manner. Phytoremediation has emerged as a highly promising approach which capitalizes on plants and their associated microorganisms for removal of pollutants from targeted sites. Plant root exudations and secondary metabolites efficiently orchestrate selective recruitment of potential PCB-degrading microbial consortia within the rhizosphere and inside plant tissues. Structural analogy between organic contaminants and secondary plant metabolites (SPMEs) renders possible uptake and subsequent degradation of pollutants by microorganisms. Present review is focused on potential role of plant root exudates and SPMEs in structuring and orchestrating remediation of PCBs within rhizosphere and inside plant tissues. Also, recent developments in tools and techniques to study remediation of organic contaminants with special reference to PCBs are addressed.

show abstract

“…Several plant species including Salix caprea, Willow, Birch, Pinus, Poplus, Solanum nigrum, Nicotiana tabacum, Armoracia rusticana, among others, are well known to harbor bacterial populations capable of degrading organic compounds especially PCBs (Leigh et al 2007 ;Ionescu et al 2009 ;Uhlik et al 2012 ). Siciliano et al ( 2001 ) showed that plants growing in xenobiotic contaminated soils have natural ability to recruit endophytes having catabolic genes (Siciliano et al 2001 ).…”

Section: Plant: Microbe Partnerships For Enhanced Phytoremediation Ofmentioning

confidence: 99%

Plant-Microbe Partnerships for Enhanced Biodegradation of Polychlorinated Biphenyls

Jha¹

2014

Plant Microbes Symbiosis: Applied Facets

View full text Add to dashboard Cite

Isolation and characterization of different plant associated bacteria and their potential to degrade polychlorinated biphenyls

Cited by 57 publications

References 21 publications

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Secondary plant metabolites and root exudates: guiding tools for polychlorinated biphenyl biodegradation

Plant-Microbe Partnerships for Enhanced Biodegradation of Polychlorinated Biphenyls

Contact Info

Product

Resources

About