Isolation and Characterization of Dehydration-Responsive Element-Binding Factor 2C (MsDREB2C) from Malus sieversii Roem.

Zhao, K.; Shen, X.; Yuan, H.; Liu, Y.; Liao, X.; Wang, Q.; Liu, L.; Li, F.; Li, T.

doi:10.1093/pcp/pct143

Cited by 8 publications

(12 citation statements)

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“…In addition, PtrDREB28 was stress-inducible by high temperature and low temperature. This finding is consistent with those of Dubouzet et al (2003) [10], who reported increased expression of an A-2 subgroup gene ( MsDREB2C ) of Malus sieversii Roem [27]. It was a major regulator of dehydration and heat shock responses in the DREB2 subgroup [7].…”

Section: Resultssupporting

confidence: 90%

Gene Structures, Classification, and Expression Models of the DREB Transcription Factor Subfamily in Populus trichocarpa

Chen

Yang

Wang

et al. 2013

The Scientific World Journal

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We identified 75 dehydration-responsive element-binding (DREB) protein genes in Populus trichocarpa. We analyzed gene structures, phylogenies, domain duplications, genome localizations, and expression profiles. The phylogenic construction suggests that the PtrDREB gene subfamily can be classified broadly into six subtypes (DREB A-1 to A-6) in Populus. The chromosomal localizations of the PtrDREB genes indicated 18 segmental duplication events involving 36 genes and six redundant PtrDREB genes were involved in tandem duplication events. There were fewer introns in the PtrDREB subfamily. The motif composition of PtrDREB was highly conserved in the same subtype. We investigated expression profiles of this gene subfamily from different tissues and/or developmental stages. Sixteen genes present in the digital expression analysis had high levels of transcript accumulation. The microarray results suggest that 18 genes were upregulated. We further examined the stress responsiveness of 15 genes by qRT-PCR. A digital northern analysis showed that the PtrDREB17, 18, and 32 genes were highly induced in leaves under cold stress, and the same expression trends were shown by qRT-PCR. Taken together, these observations may lay the foundation for future functional analyses to unravel the biological roles of Populus' DREB genes.

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Section: Resultssupporting

confidence: 90%

Gene Structures, Classification, and Expression Models of the DREB Transcription Factor Subfamily in Populus trichocarpa

Chen

Yang

Wang

et al. 2013

The Scientific World Journal

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“…PeDREB1A and PeDREB1 also have similar expression patterns in leaves, being strongly expressed under low temperature treatments and with relative expression peaking at 3 h, while under drought and salinity conditions, expression levels remained close to basal levels. Most plant resistance research has only focused on the analysis of gene expression patterns in leaves (Niu et al, 2010;Zhao et al, 2013;Liang et al, 2014), and less research has focused on gene expression in roots. The current study also examined PeDREB2A and PeDREB1A gene expression patterns in moso bamboo roots under drought, low temperature, and salinity stress, providing further understanding of gene expression patterns in different plant tissues.…”

Section: Discussionmentioning

confidence: 99%

“…For example, expression of CiDREB1A and CiDREB1B was found to be induced during low temperature adverse conditions but not in response to other abiotic stresses (Liang et al, 2014), and the BdDREB2 gene was significantly expressed in response to drought and salt stress (Zhang et al, 2014). In previous studies, a number of DREB TFs have been isolated from many plant species, including Physcomitrella patens (PpDBF1) (Liu et al, 2007), Hordeum vulgare (HvDREB1-a) (Xu et al, 2009), Medicago falcate (MfDREB1 and MfDREB1s) (Niu et al, 2010), Glycine soja (GsTIFY11b) (Zhu et al, 2012), oil palm (EABF and EABF1) (Omidvar et al, 2012), Avicennia marina (AmCBF1, 2, and 3) (Peng et al, 2013a), and Malus sieversii Roem (MsDREB2C) (Zhao et al, 2013). Interestingly, it has been found that over-expression of stress inducible DREB TFs may regulate the expression of all target genes that contain a DRE element within their promoters, resulting in transgenic plants with improved stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, it has been found that over-expression of stress inducible DREB TFs may regulate the expression of all target genes that contain a DRE element within their promoters, resulting in transgenic plants with improved stress tolerance. For example, over-expression of the BdDREB2 or PpD-BF1 genes in tobacco, the MsDREB2C or HvDREB1 genes in Arabidopsis, and the AtDREB2A gene in soybean have been demonstrated under drought, cold or salt stress, suggesting that the transgenic plants may have improved tolerance of abiotic stress (Liu et al, 2007;Xu et al, 2009;Zhao et al, 2013;Engels et al, 2013;Zhang et al, 2014). Thus, DREB genes are promising candidates in terms of molecular breeding for stress resistance in plants.…”

Section: Introductionmentioning

confidence: 99%

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Cloning and stress response analysis of the PeDREB2A and PeDREB1A genes in moso bamboo (Phyllostachys edulis)

Wu¹,

L²,

Zc³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Moso bamboo is a large woody bamboo with the highest ecological, economic, and cultural value among all bamboos in Asia. However, environmental stress influences its growth and development and limits its geographic distribution. Therefore, improving its resistance to environmental stress is extremely important. Dehydration responsive element binding (DREB) transcription factors perform an important role in the regulation of stress-related genes, enhancing the resistance of plants to abiotic stress. In the current study, two novel DREB genes, PeDREB2A and PeDREB1A (Gene ID No. PH01000046G1730 and PH01000668G0350), were isolated from moso bamboo and the sequences were identified and characterized (coding sequence lengths were 795 and 825 bp, respectively). The PeDREB2A and PeDREB1A proteins were estimated to have typical AP2/ERF domains, molecular weights of 28.96 and 28.84 kDa, and isoelectric points of 9.47 and 5.34, respectively.

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“…DREB2 homologous genes have been found in Arabidopsis, rice, canola, wheat, barley, maize and apple (Xue and Loveridge 2004;Egawa et al 2006;Qin et al 2007;Zhao et al 2013;Kudo et al 2014). Two subfamily genes, A1 (MbDREB1) and A6 (MrDREBA6) were recently identified in apple with RT-PCR method, the results showed that both were induced by multiple abiotic stress.…”

Section: Introductionmentioning

confidence: 98%

Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress

Zhao

Sun

et al. 2017

J. Plant Biochem. Biotechnol.

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DREB transcription factors regulate the expression of many abiotic-stress-responsive genes and play critical roles in improving abiotic stress tolerance in plants.In this study, the MdDREB2 transcription factors in apple (Malus domestica) were identified through bioinformatics approach, and MdFREB2 transcript levels in different tissues and abiotic stress conditions were also further studied using RT-PCR methods. Fifteen MdDREB2 transcription factors were identified in apple genome, and all contain NLS motif and AP2 domain. The phylogenetic analysis showed that MdDREB2 transcription factors were divided into 3 subtypes: subtype 1, 2, and 3. Subtype 1 included MdDREB2A, B, D, O, L, and subtype 2 had MdDREB2 N and MdDREB2M, while MdDREB2F, G, K, J, H, I, E belonged to subtype 3. Chromosome mapping analysis showed that the largest number of MdDREB2 transcription factors were found on chromosome 12 (7 genes), followed by chromosomes 1, 4 and 8 (2 genes each), and only one gene was located on chromosomes 7 and 17, respectively. Quantitative RT-PCR results demonstrated that MdDREB2 transcription factors had similar expression patterns and had higher expression levels in roots and leaves than other tissues. Besides, their expression level was increased more or less under drought and salinity stress. In summary, MdDREB2 transcription factors shared highly conserved protein structure, and were responsive to abiotic stresses, which indicated the important role against various environmental conditions.

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Isolation and Characterization of Dehydration-Responsive Element-Binding Factor 2C (MsDREB2C) from Malus sieversii Roem.

Cited by 8 publications

References 0 publications

Gene Structures, Classification, and Expression Models of the DREB Transcription Factor Subfamily in Populus trichocarpa

Gene Structures, Classification, and Expression Models of the DREB Transcription Factor Subfamily in Populus trichocarpa

Cloning and stress response analysis of the PeDREB2A and PeDREB1A genes in moso bamboo (Phyllostachys edulis)

Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress

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