Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress

Li, Huifeng; Zhao, Qiang; Sun, Xiaofen; Jia, Houzhen; Ran, Kun

doi:10.1007/s13562-017-0405-y

Cited by 5 publications

(6 citation statements)

References 31 publications

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“…Cold stress induces the expression of about 40% of drought-responsive rice genes ( Gao et al 2007 ). Overlap between the expression profiles of stress-responsive genes induced by drought and cold stress has been observed in apple ( Li et al 2017a ; Li et al 2019a ), tangerine ( Citrus) ( de Oliveira et al 2011 ), grape ( Vitis vinifera ) ( Zandkarimi et al 2015 ), Arabidopsis ( Arabidopsis thaliana ) ( Seki et al 2002 ), and other plant species. In addition, overexpression of genes encoding the transcription factor (TF) MYB121 in Malus ( Cao et al 2013 ), the antioxidant enzyme manganese superoxide dismutase (Mn-SOD) in Populus davidiana ( Populus ) ( Yu et al 2010 ), the trihelix TF family member cold-induced GT (poly [dG-dT] factor) (CIGT) from the wild tomato species Solanum habrochaites ( Yu et al 2018 ), and others improves plant tolerance to both drought and cold stresses.…”

Section: Introductionmentioning

confidence: 99%

Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

Meng

et al. 2023

Plant Physiology

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Drought and cold stresses seriously affect tree growth and fruit yield during apple (Malus domestica) production, with combined stress causing injury such as shoot shriveling. However, the molecular mechanism underlying crosstalk between responses to drought and cold stress remains to be clarified. In this study, we characterized the zinc finger transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 10 (ZAT10) through comparative analysis of shoot-shriveling tolerance between tolerant and sensitive apple rootstocks. MhZAT10 responded to both drought and cold stress. Heterologous expression of MhZAT10 in the sensitive rootstock ‘G935’ from domesticated apple (Malus domestica) promoted shoot-shriveling tolerance, while silencing of MhZAT10 expression in the tolerant rootstock ‘SH6’ of Malus honanensis reduced stress tolerance. We determined that the apple transcription factor DEHYDRATION RESPONSE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a direct regulator activating the expression of MhZAT10 in response to drought stress. Apple plants overexpressing both MhDREB2A and MhZAT10 genes exhibited enhanced tolerance to drought and cold stress, while plants overexpressing MhDREB2A but with silenced expression of MhZAT10 showed reduced tolerance, suggesting a critical role of MhDREB2A-MhZAT10 in the crosstalk between drought and cold stress responses. We further identified drought-tolerant MhWRKY31 and cold-tolerant MhMYB88 and MhMYB124 as downstream regulatory target genes of MhZAT10. Our findings reveal a MhDREB2A-MhZAT10 module involved in crosstalk between drought and cold stress responses, which may have applications in apple rootstock breeding programs aimed at developing shoot-shriveling tolerance.

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Section: Introductionmentioning

confidence: 99%

Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

Meng

et al. 2023

Plant Physiology

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“…The rootassociated bacteria are sensitive to even small changes in the pattern of compounds in the rhizosphere (Persello-Cartieaux et al, 2003), which may be altered by genetic modifications for enhancing or conferring specific traits. Although numerous studies have confirmed that DREB2s contribute greatly to the enhanced drought and salinity tolerance of a plant (Chen et al, 2009;Matsukura et al, 2010;Li et al, 2017), few studies have focused on the bacterial communities in the soil of plants modified by DREB2s (Chunmiao et al, 2015). Therefore, it is important to evaluate the influence of plants modified with DREB2 genes on hormone processes that could influence the root-associated bacterial communities.…”

Section: Introductionmentioning

confidence: 99%

Effects of Drought-Tolerant Ea-DREB2B Transgenic Sugarcane on Bacterial Communities in Soil

et al. 2020

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Drought is a major abiotic stress affecting plant growth and development. Sugarcane, a sugar crop planted in warmer climate, suffers dramatically from drought stress. Bacterial communities colonizing the rhizosphere, where roots sense drought stress initially, have been well studied for their beneficial effects on plant growth and health. The Ea-DREB2B gene cloned from the sugarcane, Saccharum arundinaceum, belongs to the DREB2 subgroup of the DREB gene family, which is involved in drought response regulation. Here, we present a detailed characterization of the rhizoplane, rhizosphere, and bulk soil bacterial communities determined using a high-sequencing approach with the transgenic (TG) sugarcane variety GN18 harboring the drought-tolerant Ea-DREB2B gene and its isogenic wild-type (WT) variety FN95-1702 under the same environmental conditions. In addition, the total carbon (TC), total nitrogen (TN), and total phosphorus (TP) contents in each soil area were compared to explore the relationship between bacterial alteration in the TG and WT plants and environmental factors (TC, TN, TP, C:N, C:P, and N:P). Our results showed that the bacterial communities in the rhizosphere and rhizoplane of TG sugarcane were more similar and perfectly correlated with the environmental factors than those of the WT. This suggested that the bacterial communities of the TG plants were altered in response to the changes in root exudates. The results of our study suggest that the change in soil environment caused by transgenic sugarcane alters soil bacterial communities.

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“…There are several aspects of the plant response to drought, such as sensing stress, activating systemic signaling pathways, and genetically regulating the responses (Zolla et al, 2013). Many genes involved in plant responses to drought have been identified, and some of these have been effectively used to improve drought tolerance (Gosti et al, 1995;Iuchi et al, 2001;Li et al, 2017) by developing new drought-tolerant crop varieties through genetic modifications to increase crop productivity and reduce costs (Romão-Dumaresq et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Further, fungal community structure and diversity are affected by many environmental variables (Yang et al, 2017). Although DREB2s are known to contribute greatly to enhance drought and salinity tolerance in plants (Chen et al, 2009;Matsukura et al, 2010;Li et al, 2017), few studies have focused on the fungal communities in the soil of TG plants harboring DREBs (Yu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane

et al. 2020

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Drought limits crop productivity, especially of sugarcane, which is predominantly grown in the subtropical parts of China. Soil microbes perform a wide range of functions that are important for plant productivity and responses to drought stress, and fungi play an important role in plant-soil interactions. The Ea-DREB2B gene of sugarcane, Saccharum arundinaceum, is involved in regulating the response to drought stress. In this study, fungal communities of the transgenic (TG) sugarcane variety GN18, harboring the drought-tolerant gene Ea-DREB2B and its corresponding non-TG wild-type (WT) variety, FN95-1702, were investigated in three soil compartments (rhizoplane, rhizosphere, and bulk soil) by assessing the internal transcribed spacer region using Illumina MiSeq. As the soil microbial community is also affected by various environmental factors, such as pH, carbon availability, and soil moisture, we determined the total carbon (TC), total nitrogen (TN), and total phosphorus (TP) contents in the rhizoplane, rhizosphere, and bulk soil compartments to explore the associations between soil fungal communities and host plant characteristics. The differences between the soil fungal communities of TG and WT plants were detected. The alpha diversity of TG fungal communities was more correlated to environmental factors than the beta diversity. The abundance of operational taxonomic units (OTUs) enriched in TG root-related area was far more than that in the root-related area of WT plants. Thereinto, more saprotrophs were enriched in the TG root-related area, indicating altered niches of fungal guilds around TG roots. These results revealed that host plant genotype did play a key role for strengthening plant-fungi interaction and enhancing beneficial fungal function in the root-related area (rhizoplane and rhizosphere) of TG sugarcane in order to respond to drought stress.

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Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress

Cited by 5 publications

References 31 publications

Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

Effects of Drought-Tolerant Ea-DREB2B Transgenic Sugarcane on Bacterial Communities in Soil

Response of Soil Fungal Community to Drought-Resistant Ea-DREB2B Transgenic Sugarcane

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