Isolation and characterization of collagen from the skin of Brama australis

Sionkowska, Alina; Kozłowska, Justyna; Skorupska, Małgorzata; Michalska, Marta

doi:10.1016/j.ijbiomac.2015.07.032

Cited by 49 publications

(29 citation statements)

References 29 publications

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“…Yields for F1 (ASC) and F2 (PSC) were similar, between 2.3 ± 0.39 and 2.0 ± 0.29% on a wet weight basis, respectively, while the yield of F3 (ASC from bones) was considerably lower (0.2 ± 0.09%). Similar low yields (1.5%, on a wet weight basis) were reported for the acid soluble collagen (ASC) from the skin of Brama australis, also a fish from the Perciformes order (Sionkowska et al 2015).…”

Section: Collagen Extraction: Yield and Characterizationsupporting

confidence: 65%

Characterization and potential strategies for the valorisation of the Southwest Atlantic butterfish (Stromateus brasiliensis)

Ramilo-Fernández

Sotelo

2020

J Food Sci Technol

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The waste of fish resources constitutes a serious environmental problem that must be avoided. The valorisation of by-catch species and decreasing the discard rate constitute a more efficient and sustainable use of these marine biomasses. In this work, we characterize and propose different potential uses for Stromateus brasiliensis, another frequently discarded (C 90%) and poorly studied by-catch species captured in the South Atlantic Ocean (FAO 41) by trawler fishing fleets. Furthermore, in the case of this species, freezing and frozen storage of the whole fish is the only strategy currently employed for its exploitation. The results revealed that muscle from S. brasiliensis presented a high content of polyunsaturated fatty acids (20.34%) and that the concentrations of both total diacyl glyceryl ethers (2.41%) and heavy metals (Hg 0.038, Pb 0.006 and Cd 0.018 mg/kg) were below the established limits for safe human consumption. Likewise, the protein hydrolysates proved to be a good source of amino acids for human consumption or animal feeding. Minced muscle blocks could be made by a mechanical separation process of the flesh, and the composition of minced muscle did not differ much from that of the whole fish. Furthermore, this process allows the incorporation of cryoprotectants and antioxidants to extend the frozen shelf life of this fatty fish. An extraction process from mechanically mixed skin and bones yielded a good source of collagen that should not be neglected.

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Section: Collagen Extraction: Yield and Characterizationsupporting

confidence: 65%

Characterization and potential strategies for the valorisation of the Southwest Atlantic butterfish (Stromateus brasiliensis)

Ramilo-Fernández

Sotelo

2020

J Food Sci Technol

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“…It is a group with specific function to form collagen. The functional groups in sea cucumber (H. leucospilota) are different from other collagens, such as those found in S. variegatus, Oreochromis niloticus, Thunnus albacares, and puffer fish, namely amide A, B, I, II and II (Sionkowska et al, 2015;Zhong et al, 2015).…”

Section: Resultsmentioning

confidence: 94%

“…The body wall of sea cucumber was soaked in destilled water for 1 h. The dissected body wall of sea cucumber was soaked in 0.1 M NaOH solution for 6 h, then soaked in 0.1 M pH 8.0 Tris-HCl for 24 h, washed using pH 7.0 double destilled water, and then stirred using 0.5 M acetic acid for 48 and 72 h. The filtrates were centrifuged under 8000 rpm speed for 20 min, salted out using 0.9 M NaCl for overnight, and then centrifuged under 8000 rpm speed for 20 min for obtaining deposits. The deposits were dialyzed by using a dialysis bag and 0.1 M acetic acid as medium under a temperature of 4°C for 12 h (Sadowska et al, 2003;Sionkowska et al, 2015;Zhong et al, 2015). Then, the medium was replaced with destilled water, and then dialyzed for a similar period.…”

Section: Isolation Of Crude Collagenmentioning

confidence: 99%

In vitro anti-tyrosinase and anti-elastase activity of collagen from sea cucumber (Holothuria leucospilota)

Abdillah¹,

Wijiyanti²,

Setiawan³

et al. 2017

Afr. J. Biotechnol.

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Collagen is the main structural component of connective tissues in vertebral and invertebral animal tissues and organs. The collagen isolated from the body wall of sea cucumber (Holothuria lecospilota) can be used in the pharmaceutical field. The research aimed at isolating collagen from the sea cucumber body wall and testing the anti-tyrosinase and anti-elastase activity. This was done by isolation of collagen by soaking the body wall of sea cucumber using Tris-HCl-EDTA 0.1 M and dialyzing using 0.5 M acetic acid. The step was followed by characterization of collagen using FT-IR spectrophotometer and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The resulting collagen was tested for anti-tyrosinase and anti-elastase in vitro. The research showed that the resulting collagen was of the amide group with molecular weight of 166.43 and 138.35 kDa, showing α1 and α2 chains. Anti-tyrosinase activity test showed an IC 50 value of 1.20 mg/mL, while anti-elastase activity test showed an IC 50 value of 125 µg/mL. The collagen isolated from the sea cucumber has weak anti-tyrosinase and moderate anti-elastase activity.

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“…The skin of fish contains a high content of collagen, which can reach up to 30% of dry mass (Sun, Hou, Li, & Zhang, ). Thus, it is often used as ingredient for collagen and collagen peptides extraction in biological studies (Jeong, Venkatesan, & Kim, ; Senaratne, Park, & Kim, ; Sionkowska, Kozlowska, Skorupska, & Michalska, ). A classical protein sequence of collagen is the cyclic arrangement of Gly‐X‐Y within its helical domain, in which X and Y positions are often proline and hydroxyproline, and they are the most abundant content among various amino acids accounting for about 25%.…”

Section: Discussionmentioning

confidence: 99%

Bone‐strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus

Wang

et al. 2020

Food Science & Nutrition

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Fish processing produces a lot of by‐products highly containing large amount of proteins which mainly consist of collagen, implying great potential value for application as nutraceutical ingredients. In present study, two kinds of sharks, Chiloscyllium plagiosum and Mustelus griseus, were used as raw material to gain three kinds of “compound peptides” (CPs) by enzymolysis, FCP (CPs from the flesh of C. plagiosum), SCP (CPs from the skin of C. plagiosum), and SMG (CPs from the skin of M. griseus). According to a series of constituent analysis, the molecule weights of FCP, SCP, and SMG were under 800 Da; amino acids composition analysis of FCP, SCP, and SMG showed that there were high glycine, proline, and hydroxyproline and low cysteine contents in SCP and SMG, which is the characteristic of collagen peptides; their total protein contents were 87.500%, 91.875%, and 95.625%, respectively; and heavy metal contents of CPs were all beneath national standards. After three kinds of CPs were administrated intragastrically to C57BL/6 mice at a total dosage of 15 g/kg, bone‐strengthening effects of SCP and SMG were manifested by osteoblasts activity promotion, bone mineral density (BMD) increase, and marrow adipocyte number decrease, yet nonsignificant effects were shown in FCP group. No index showed toxicity of SCP and SMG in subacute toxicology trial, indicating their safety as functional foods. Herein, industrial application foundation of the skins from these two sharks was explored but more efforts should subsequently be implemented for further exploitation.

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Isolation and characterization of collagen from the skin of Brama australis

Cited by 49 publications

References 29 publications

Characterization and potential strategies for the valorisation of the Southwest Atlantic butterfish (Stromateus brasiliensis)

Characterization and potential strategies for the valorisation of the Southwest Atlantic butterfish (Stromateus brasiliensis)

In vitro anti-tyrosinase and anti-elastase activity of collagen from sea cucumber (Holothuria leucospilota)

Bone‐strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus

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