Isolation and characterization of cDNAs encoding Ars2 and Pasha homologues, two components of the RNA interference pathway in Litopenaeus vannamei

Chen, Yihong; Li, Zhao; Jia, Xiao-Ting; Li, Xiaoyun; Li, Chao Zheng; Yan, Hui; Weng, Shaoping; He, Jianguo

doi:10.1016/j.fsi.2011.11.032

Cited by 27 publications

(16 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In L. vannamei, only Argonaute-2 is responsive to dsRNA [191]. Hence, it was thought that Argonaute-1 operates through the miRNA pathway in shrimps [195]. As in arthropods, the miRNA pathway is associated with crustacean antiviral defence [196, 197].…”

Section: Resultsmentioning

confidence: 99%

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Lai

Aboobaker

2017

BMC Genomics

View full text Add to dashboard Cite

BackgroundGrowing global demands for crustacean food crop species have driven large investments in aquaculture research worldwide. However, large-scale production is susceptible to pathogen-mediated destruction particularly in developing economies. Thus, a thorough understanding of the immune system components of food crop species is imperative for research to combat pathogens.ResultsThrough a comparative genomics approach utilising extant data from 55 species, we describe the innate immune system of the class Malacostraca, which includes all food crop species. We identify 7407 malacostracan genes from 39 gene families implicated in different aspects of host defence and demonstrate dynamic evolution of innate immunity components within this group. Malacostracans have achieved flexibility in recognising infectious agents through divergent evolution and expansion of pathogen recognition receptors genes. Antiviral RNAi, Toll and JAK-STAT signal transduction pathways have remained conserved within Malacostraca, although the Imd pathway appears to lack several key components. Immune effectors such as the antimicrobial peptides (AMPs) have unique evolutionary profiles, with many malacostracan AMPs not found in other arthropods. Lastly, we describe four putative novel immune gene families, potentially representing important evolutionary novelties of the malacostracan immune system.ConclusionOur analyses across the broader Malacostraca have allowed us to not only draw analogies with other arthropods but also to identify evolutionary novelties in immune modulation components and form strong hypotheses as to when key pathways have evolved or diverged. This will serve as a key resource for future immunology research in crustacean food crops.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3769-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Lai

Aboobaker

2017

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Primitively, researchers discovered that virus replication was significantly inhibited by dsRNA [99]–[103] and siRNA [104]–[106] of specific genes, revealing an RNAi mechanism against virus infection might exist in shrimp. Later, the genes encoding Argonaute, Dicer and other members of RNAi pathway were obtained in shrimp and evidence showed that they were involved in antiviral process via RNAi mechanism [107]–[110]. Therefore, more and more researchers deemed that application of RNAi machinery was an efficiently therapeutic method against virus infection [111]–[115] in shrimp aquaculture.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection

et al. 2013

View full text Add to dashboard Cite

Previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (WSSV) infection in crustacean. However, little information is available in relation to underlying mechanisms of host responses during the WSSV acute infection stage in naturally infected shrimp. In this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. A total of 64,188,426 Illumina reads, including 31,685,758 reads from the latent infection group and 32,502,668 reads from the acute infection group, were generated and assembled into 46,676 unigenes (mean length: 676 bp; range: 200–15,094 bp). Approximately 24,000 peptides were predicted and classified based on homology searches, gene ontology, clusters of orthologous groups of proteins, and biological pathway mapping. Among which, 805 differentially expressed genes were identified and categorized into 11 groups based on their possible function. Genes in the Toll and IMD pathways, the Ras-activated endocytosis process, the RNA interference pathway, anti-lipopolysaccharide factors and many other genes, were found to be activated in shrimp from latent infection stage to acute infection stage. The anti-bacterially proPO-activating cascade was firstly uncovered to be probably participated in antiviral process. These genes contain not only members playing function in host defense against WSSV, but also genes utilized by WSSV for its rapid proliferation. In addition, the transcriptome data provides detail information for identifying novel genes in absence of the genome database of shrimp.

show abstract

“…In L. vannamei , only Argonaute-2 is responsive to dsRNA (Labreuche et al, 2010). Hence, it was thought that Argonaute-1 operates through the miRNA pathway in shrimps (Chen et al, 2012). As in arthropods, the miRNA pathway is associated with crustacean antiviral defence (Lu et al, 2009; Fullaondo et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

The innate immune systems of malacostracan crustaceans exhibit both conserved and evolutionarily distinct components

Lai

Aboobaker

2016

Preprint

View full text Add to dashboard Cite

Isolation and characterization of cDNAs encoding Ars2 and Pasha homologues, two components of the RNA interference pathway in Litopenaeus vannamei

Cited by 27 publications

References 46 publications

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Transcriptome Analysis on Chinese Shrimp Fenneropenaeus chinensis during WSSV Acute Infection

The innate immune systems of malacostracan crustaceans exhibit both conserved and evolutionarily distinct components

Contact Info

Product

Resources

About