Isolation and characterization of biosurfactant-producing Alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis

Olivera, Nelda Lila; Nievas, Marina L.; Lozada, Mariana; Prado, Guillermo del; Dionisi, Hebe M.; Siñeríz, Faustino

doi:10.1016/j.resmic.2008.09.011

Cited by 52 publications

(28 citation statements)

References 28 publications

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“…It is surprising that this phylotype was found only in the tidal biofilms and was not in the upper 1.5 cm of sediment. The Alcanivorax sequences found in the upper 1.5 cm of sediment were most closely related (98 and 97% 16S rRNA identity, respectively) to strain GPM2532, isolated from a mangrove sediment (5), and strain PA2, from 0-to 3-cm-deep intertidal sediment (34), which is indicative of a phylotype preferentially adapted to living within muddy sediments. Nutrient amendments were previously suggested to select for different phylotypes of Alcanivorax (22).…”

Section: Discussionmentioning

confidence: 87%

Central Role of Dynamic Tidal Biofilms Dominated by Aerobic Hydrocarbonoclastic Bacteria and Diatoms in the Biodegradation of Hydrocarbons in Coastal Mudflats

Coulon

Chronopoulou

Fahy

et al. 2012

Appl Environ Microbiol

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ABSTRACTMudflats and salt marshes are habitats at the interface of aquatic and terrestrial systems that provide valuable services to ecosystems. Therefore, it is important to determine how catastrophic incidents, such as oil spills, influence the microbial communities in sediment that are pivotal to the function of the ecosystem and to identify the oil-degrading microbes that mitigate damage to the ecosystem. In this study, an oil spill was simulated by use of a tidal chamber containing intact diatom-dominated sediment cores from a temperate mudflat. Changes in the composition of bacteria and diatoms from both the sediment and tidal biofilms that had detached from the sediment surface were monitored as a function of hydrocarbon removal. The hydrocarbon concentration in the upper 1.5 cm of sediments decreased by 78% over 21 days, with at least 60% being attributed to biodegradation. Most phylotypes were minimally perturbed by the addition of oil, but at day 21, there was a 10-fold increase in the amount of cyanobacteria in the oiled sediment. Throughout the experiment, phylotypes associated with the aerobic degradation of hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs) (Cycloclasticus) and alkanes (Alcanivorax,Oleibacter, andOceanospirillalesstrain ME113), substantively increased in oiled mesocosms, collectively representing 2% of the pyrosequences in the oiled sediments at day 21. Tidal biofilms from oiled cores at day 22, however, consisted mostly of phylotypes related toAlcanivorax borkumensis(49% of clones),Oceanospirillalesstrain ME113 (11% of clones), and diatoms (14% of clones). Thus, aerobic hydrocarbon biodegradation is most likely to be the main mechanism of attenuation of crude oil in the early weeks of an oil spill, with tidal biofilms representing zones of high hydrocarbon-degrading activity.

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Section: Discussionmentioning

confidence: 87%

Central Role of Dynamic Tidal Biofilms Dominated by Aerobic Hydrocarbonoclastic Bacteria and Diatoms in the Biodegradation of Hydrocarbons in Coastal Mudflats

Coulon

Chronopoulou

Fahy

et al. 2012

Appl Environ Microbiol

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“…Alcanivorax sp. produces cell-bound and extracellular forms of biosurfactant; these surface-active compounds have an affinity for oil droplet suspended in the water phase [23,[51][52][53][54][55][56]. The function of exported neutral lipids and of the meshworks have to be discussed in this context.…”

Section: Discussionmentioning

confidence: 99%

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Lange

Tenberge²,

Robenek

et al. 2010

Euro J Lipid Sci & Tech

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This study presents novel information useful for addressing the question how species of the genus Alcanivorax discharge triacylglycerols (TAG) and/or wax esters (WE). The observed structures were referred as ''blebs'' according to Gauthier et al. [1] to avoid confusion with other discharging phenomena. The cells were aerobically cultivated on solid media and not in liquid media to maintain the cells in the native state, and were investigated by transmission electron microscopic (TEM) and scanning electron microscopic (SEM) methods to document the surface structures of the cells. The phenomenon of lipid export could be allocated to three phases: phase I: protrusion formation of the cell membrane occurred; phase II: discharging progressed further with blebs becoming larger; and phase III: the blebs at the cell surface were separated from the cells. Using freeze-fracture micrographs by TEM, vesicle experiments and TLC, we have shown that the blebs contained TAG and WE. The results shown in this study will support further research to unravel the unknown discharging mechanism. In addition, the formation of an extensive extracellular matrix was observed by SEM.

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“…The genetic evidence of a possible aromatic ring meta-cleaving pathway was reported in aquatic environment (Zhang et al 2008). Some novel genes of Alkane hydroxylase were found in two Alcanivorax isolates (Olivera et al 2009). These all confirmed the possibility that the positive mutants could enhance the degrading capacity through the genetic mutagenesis.…”

Section: Assay Of the Mutant Strainsmentioning

confidence: 93%

Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation

Chen

Yang

Huang

et al. 2010

World J Microbiol Biotechnol

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This study focused on enhancing the bioremediation of soil contaminated with viscous oil by microorganisms and evaluating two strategies. Construction of microbial consortium and ultraviolet mutation were both effective applications in the remediation of soil contaminated with viscous oil. Results demonstrated that an interaction among the microorganisms existed and affected the biodegradation rate. Strains inoculated equally into the test showed the best remediation, and an optimal microbial consortium was achieved with a 7 days' degradation rate of 49.22%. On the other hand, the use of ultraviolet mutation increased one strain's degrading ability from 41.83 to 52.42% in 7 days. Gas chromatography and mass spectrum analysis showed that microbial consortium could treat more organic fractions of viscous oil, while ultraviolet mutation could be more effect on increasing one strain's degrading ability.

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Isolation and characterization of biosurfactant-producing Alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis

Cited by 52 publications

References 28 publications

Central Role of Dynamic Tidal Biofilms Dominated by Aerobic Hydrocarbonoclastic Bacteria and Diatoms in the Biodegradation of Hydrocarbons in Coastal Mudflats

Central Role of Dynamic Tidal Biofilms Dominated by Aerobic Hydrocarbonoclastic Bacteria and Diatoms in the Biodegradation of Hydrocarbons in Coastal Mudflats

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation

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